Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	465,005	G→A	V181V (GTC→GTT)	queC ←	7‑cyano‑7‑deazaguanine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	465,005	0	G	A	90.9%	19.9 / NA	11	V181V (GTC→GTT)	queC	7‑cyano‑7‑deazaguanine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (7/3);  minor base C (1/0);  total (8/3)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.30e-01

CTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATT  >  NC_000913/464821‑465224                                                                                                                                                                                         |                                                                                                                                                                                                                            ntCAAGCCGCATACGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAgc                                                                                                                                                           >  1:87507/2‑251 (MQ=255)   cAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAgcg                                                                                                                                                          <  1:193879/250‑1 (MQ=255)         gCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGAACTAAATCCAGTGTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTc                                                                                                                                                   <  1:235833/251‑1 (MQ=255)                                           cGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATCATGATTCAAACCGTTGGCGCGTAAATTACATGCAGCACAATGACCGCAAACGTCGCCTTTAAAGCCGCTATAGCAGGTCCACGTTTCGTTAAGAAATAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCTTTTCAAAACGAATATCTTTCGCCATGCCCAAACttccg                                                                                                                 >  1:182048/1‑247 (MQ=255)                                             gTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGAATCAAACCGTTGGCGAGAAAATTACATTACACACAAAGACCGCAACCGTCGCCTTTTAAGACGTTAAAACAGGTCAACTGTTCGCTACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGATTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAAATGAccgc                                                                                                               >  1:198324/1‑248 (MQ=255)                                                                                        gATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTGACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCTCCCAGGTTTCCGCGTTATCAATCCGCAGCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAg                                                                                                      <  1:202508/217‑1 (MQ=255)                                                                                                  cAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAAt                                                          >  1:269859/1‑251 (MQ=255)                                                                                                             cgcgTAAATCACATGCCGCACAATGACCGAAACCGACGCCTTTAAAGCAGTTATAGCAGGTCACCGTTTCGTTACGAACTAAATCCAGTTTGCCGTCATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTc                                                                                                                                                   >  1:290956/1‑151 (MQ=255)                                                                                                              gcgTAAATTACATGCCGCCCAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGc                                                 >  1:95807/1‑248 (MQ=255)                                                                                                                                       ccGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGCACTAAATCCAGTTTGACGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTAAAAACGAATATCTTTCGCCATGCCCAAACTGACgg                                                                                                                >  1:330196/1‑160 (MQ=255)                                                                                                                                                          aGCCGTTATAGCAGGTCAACGTTTCGTTACGAACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATAtt  >  1:13356/1‑251 (MQ=255)                                                                                                                                                                                         |                                                                                                                                                                                                                            CTCAAGCCGCATCCGGCGACACCCGGAATAATTACCTCAACCCGGTTTTCTGCTTCATCGCTGCCATCACCGTCGGTTTATCGGCCAGATAATGATTCAAACCGTTGGCGCGTAAATTACATGCCGCACAATGACCGCAACCGTCGCCTTTAAAGCCGTTATAGCAGGTCAACGTTTCGTTACGGACTAAATCCAGTTTGCCGTAATAATCTGCCAGCGCCCAGGTTTCCGCTTTATCAATCCACATCAGCGGCGTTTCAAAACGAATATCTTTCGCCATGCCCAAACTGACGGCATGGTTTAGTGCTTTCACAAACTCATCGCGGCAATCCGGGTAGCCGGAGAAATCCGTTTCGCAGACGCCAGTAATTACGGCTTCTGCTTTTACCTGATACGCATATATT  >  NC_000913/464821‑465224

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑