| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 433,643 | C→T | A63A (GCC→GCT) | ribD → | fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 433,643 | 0 | C | T | 86.7% | 22.8 / ‑4.8 | 15 | A63A (GCC→GCT) | ribD | fused diaminohydroxyphosphoribosylaminopyrimidine deaminase/5‑amino‑6‑(5‑phosphoribosylamino)uracil reductase |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (4/9); minor base A (1/1); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
GCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGA > NC_000913/433423‑433843 | gCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCTAAAGGGGCGACCGCCTATGTCACACTCGa > 1:330647/1‑250 (MQ=255) gcgcCGGGAGGACTAAGCCTTTCTGGACGAGTATTAAATGGCGGGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCAGCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCTAAAATGTGGGTGAAGGTTACCACCAACTTGCGGTTGACCCAAATGCCGAAGTACACGCGTTGCGTATGGCGGGTTAAAAAGCTAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCcatc < 1:253663/251‑1 (MQ=255) aGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCACCGTGCGGGTGAACCACATGCCGAAGTACACGCGTGGCGTATGGCGGGTGAAAAAGCTAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCtgt < 1:113438/251‑1 (MQ=255) gTATTACATTGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACAAACGTGCGGGTGAACCACATGCCGACGTACACGCGTTGCGTATGGCGGGTGAAAAAGCTAAAGGGGCGACCCCCTATGTCACCCCCGAACCCCGTAGCCATCATGGTCGTACGCCCCCGCGCTGTGACGCAc > 1:337845/1‑251 (MQ=255) gcTAAAGCGGGCGCAACGAGGACGTGTTACCACGCACCCCAACCCGAATGTCGGGGGCGCCATTGTCAAAGATGCCGAAATTGTCGGTGAAGGGTACCACCGACGTCAGGGGGAACCACAGGCCGAAGTACACGCGTGGCGGAGGGCGGGGGATAAACCTAAAGGTCCGACCGCCGATGGCACACACGAGCCCGTTAGCCATCAGGGTCTGACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAg < 1:348245/251‑1 (MQ=255) ntAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCTAAAGGTGCGAc > 1:103320/2‑170 (MQ=255) gCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCTAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGc < 1:18088/217‑1 (MQ=255) cAACGAGGACGTTTTACCACGAATCCCAACCCGAATGTCGGGGGGGTCATGGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGACCCACATGCCGAAGTACACGCGTGGCGTAGGGCGGGGGAAAAAGCTAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTgn < 1:42470/251‑2 (MQ=255) gggTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGAGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGGGAAAAAGCTAAAGGTGGGACCCCCTATGTCACAATCGAAACCTGTAg > 1:308924/1‑146 (MQ=255) cATTGTCAAAGAGGGCGAAATTGGCGGTGAAGGTTCCCCCCAACTTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGGGGGTGAAAAAGCTAAAGGTGCGACCGCCTATGTCACACTCGATCCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTAcn < 1:69084/251‑2 (MQ=255) gTCAAAGATGGCGAAATTGTCAGTGAAGTTTACCACCAACGTGCGGGTGAACCACATGCCGAATTACCCTCTTGGCGTATGGCGGGTGAAAAAGCAAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGTTCGTACGCCACCGTGCTGTGACGCGCGCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGAtn < 1:43720/207‑2 (MQ=255) gATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCTAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAgcg < 1:7756/183‑1 (MQ=255) aTGGCGAAATTGTCGGTGAAGGTTACCCCCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTAGGGCGGGTGAAAAAGCTAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACan < 1:92428/251‑2 (MQ=255) ggTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCTAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAg < 1:177458/166‑1 (MQ=255) tGAACCACATGCCGAAGTACACGCGTTGCGTATGCCGGGCGAAAAAGCAAAAGGAGCGACCGCCTATGTCACACTAGAACACTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGGTGCCTCGATGCAAGATCCTAACCCGCAGGACGCTGGGCGTGGCATTTCCCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGa > 1:120084/1‑249 (MQ=255) aaaaGNNAANNNTGNNACCGCCTATGTCACACTCGAACCNTGTAGCCCTCATGGTCGTACGCCACCGTGNTNTNACGCNNTCANCGCCGCTGGCNTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAgg > 2:256874/1‑137 (MQ=255) | GCGAAGAGATCGCGCGCCTGGAGGACTAAGCCGTGCAGGACGAGTATTACATGGCGCGGGCGCTAAAGCTGGCGCAACGAGGACGTTTTACCACGCATCCCAACCCGAATGTCGGGTGCGTCATTGTCAAAGATGGCGAAATTGTCGGTGAAGGTTACCACCAACGTGCGGGTGAACCACATGCCGAAGTACACGCGTTGCGTATGGCGGGTGAAAAAGCCAAAGGTGCGACCGCCTATGTCACACTCGAACCCTGTAGCCATCATGGTCGTACGCCACCGTGCTGTGACGCACTCATCGCCGCTGGCGTAGCGCGCGTGGTTGCCTCGATGCAAGATCCTAACCCGCAGGTCGCTGGGCGTGGACTTTACCGTCTGCAACAGGCTGGCATTGACGTCAGCCACGGCCTGATGATGAGTGA > NC_000913/433423‑433843 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |