Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	431,912	C→T	W34* (TGG→TGA)	tsx ←	nucleoside‑specific channel‑forming protein Tsx

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	431,912	0	C	T	100.0%	34.1 / NA	14	W34* (TGG→TGA)	tsx	nucleoside‑specific channel‑forming protein Tsx
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/5);  total (9/5)

CCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCACCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTTCATATGTATGCCACTGTTTGAAAATCCCTTGCGGGAGTGAAAAAGGCGCAAATTGCGTTTCTAAATATTTCGTATACAGAGGAGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGT  >  NC_000913/431730‑432155                                                                                                                                                                                       |                                                                                                                                                                                                                                                    ccGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGGCGAACCAGTCTTTTTTAGTGAATGCTGCGTACTCAAGGTAGGTATCGTTGCGGAGCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTGTTCAGCTGCTTTTACAGTAAAAGACGAAGAGAGCGCCan                                                                                                                                                                                 <  1:86905/251‑2 (MQ=255)     ggTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTAcc                                                                                                                                                                             >  1:189832/1‑251 (MQ=255)                       cATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGCACCAGTCTTTTTTAGCGAATGCTTCGTACTCCAGGTAGGTATCGTTGCGGATCTGCGGTCCGCAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAACGCCGAAAAGAGCGCCAGTACCTCACCGGCTGCCAGTAAt                                                                                                                                                           >  1:334926/1‑251 (MQ=255)                                       gaagaaTACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTAGCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATATCTTCCGACAACGTTAACGCTCTGGGGCCATCAGTCGGAAAGATGCTGCGGTTTGTCTTTTTCAGCTGTGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCAcc                                                                                                                                                                        <  1:304299/222‑1 (MQ=255)                                                                ccATAGCAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTTCATATGTATGCCACTGTTTGAAAATCCCTTGCg                                                                                                                  >  1:101320/1‑251 (MQ=255)                                                                           naaCCAGTCTTTTTTAGCGACTGCTTCGTACTCAAGGTAGGTATCGTTGAGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGAc                                                                                                                                                                                               >  1:104418/2‑163 (MQ=255)                                                                                                        aCTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTGCATATGTATGCCACTGTTTGAAAATCCCTTGCGGGAGTGAAAAAGGCGCAAATTGCGTTTCTAAATATTTCg                                                                           <  1:82145/250‑1 (MQ=255)                                                                                                          tCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTTCATATGTATGCCACTGTTTGAAAATCCCTTGCGGGAGTGAAAAAgg                                                                                                     <  1:146538/222‑1 (MQ=255)                                                                                                              ggtaggtaTCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTGGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTGCATATGTATGCCACTGTTTGAAAATCCCGTGCGGGATTGAAAAAGGCGCAAATTGCGTTTCTAAATATTTCGTATACag                                                                    <  1:45234/251‑1 (MQ=255)                                                                                                                               aTCTGCGGTCCGAAACGGGGGTGATCGCTTCCGACAACGTTAACGCTCTGGTGCCATCCGTCGGAAAGATACTACGGTTTGTCGTTTTCAGCTGCGTAGACAGTAAAAGACGAAGAGAGCGCAAGTACCGCACCGGCTGCCCGTAATGTTTTTTTCATATGTATGCCACTGTTTGAAAATCCCTTGCGGGAGTGAAAAAGGCGCAAATTGCGTTTCTAAATATTTCGTATACAGAGGAGCCTATTATaaa                                                    >  1:310826/1‑250 (MQ=255)                                                                                                                                                ggTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCATACGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTTCATATGTATGCCACTGTTTGAAAATCCCTTGCGGGAGTGAAAAAGGCGCAAATTGCGTTTCTAAATATTTCGTATACAGAGGAGCCTATTATAAAAATCATTGCTCACaaaa                                   >  1:194002/1‑250 (MQ=255)                                                                                                                                                   gtgATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTTCATATGTATGCCATTGTTTGAAAATCCCTTGCGGGAGTGAAAAAGGCGCAAATTGCGTTTCTAAATATTTCGTATACAGAGGAGCCTATTATAAAAATCATTGCTCACAAAAatat                               >  1:351923/1‑251 (MQ=255)                                                                                                                                                     gATAGCTTCCGACAACGTTAACGCTCTGGTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTTCATATGTATGCCACTGTTTGAAAATCCCTTGCGGGAGTGAAAAAGGCGCAAATTGCGTTTCTAACTATTTCGTATACAGAGGAGCCTATTATAAAAATCATTGc                                           >  1:8560/1‑237 (MQ=255)                                                                                                                                                                                ngTGCCATCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTTCATATGTATGCCACTGTTTGAAAATCCCTTGCGGGAGTGCAAAAGGCGCAAATTGCGTTTCTAAATATTTCGTATACAGAGGAGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGt  >  1:72755/2‑251 (MQ=255)                                                                                                                                                                                       |                                                                                                                                                                                                                                                    CCGTGGTTCCAGATACCTTTAGCATCGGAGTTACCGCCGAAGAATACCGGCGCATCCGCATAACCATAGAAGTCGAACCAGTCTTTTTTAGCGAATGCTTCGTACTCAAGGTAGGTATCGTTGCGGATCTGCGGTCCGAAACGGGTGTGATAGCTTCCGACAACGTTAACGCTCTGGTGCCACCAGTCGGAAAGATACTGCGGTTTGTCGTTTTCAGCTGCGTTGACAGTAAAAGACGAAGAGAGCGCCAGTACCGCACCGGCTGCCAGTAATGTTTTTTTCATATGTATGCCACTGTTTGAAAATCCCTTGCGGGAGTGAAAAAGGCGCAAATTGCGTTTCTAAATATTTCGTATACAGAGGAGCCTATTATAAAAATCATTGCTCACAAAAATATGTTTCGTTTCACAGTTCTATCATTTACGT  >  NC_000913/431730‑432155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑