Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	421,027	C→T	R14R (CGC→CGT)	proY →	putative transporter ProY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	421,027	0	C	T	100.0%	65.5 / NA	24	R14R (CGC→CGT)	proY	putative transporter ProY
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/15);  total (9/15)

GGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTG  >  NC_000913/420805‑421263                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              ggCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTc                                                                                                                                                                                                                     <  1:96496/249‑1 (MQ=255)            gTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCGCCAGCGCTCACTAAATCACTGAACATTTGGTTTAACCACGGGGCTGCGATGCCCCGTGGTTGTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAg                                                                                                                                                                                                                   <  1:130023/240‑1 (MQ=255)                ggCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGc                                                                                                                                                                                                    <  1:7010/251‑1 (MQ=255)                 gCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGc                                                                                                                                                                                                           <  1:11742/243‑1 (MQ=255)                                 cAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGc                                                                                                                                                                                   >  1:159136/1‑250 (MQ=255)                                                    ggCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGcc                                                                                                                                                                <  1:145635/250‑1 (MQ=255)                                                                        cgGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGt                                                                                                                                                                                                                       >  1:99623/1‑176 (MQ=255)                                                                         ggCAGGTCGTCAGGTGACCTCCAGCGCTCACTAACTCACTGAACATTTGTTTTAACCAAGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAACTGGCCGGTCCGAGCGTGTTGTTGGCCt                                                                                                                                          >  1:306433/1‑251 (MQ=255)                                                                              gtcgtcAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCAGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACACCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTAtatt                                                                                                                                     >  1:238797/1‑251 (MQ=255)                                                                                 gtcAGGTGACCTCCAGCGCTCACTAACTCACTGCACATTTGTTTTAACCACGGGGCTGCGATGACCAGTGGTTTTTTATTGTGTTGA‑AGGTTAGGAATTGATGGAAAGAAAGAACAAGCTACAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTAtatt                                                                                                                                     >  1:289694/1‑247 (MQ=255)                                                                                      gTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCGAAAGCGTGGGCTAAGGACCCGTCACATACGCTTTATGTAACTGGGTTCAGCAATTGGCACCGGGc                                                                                                                                                                                                    <  1:227579/181‑1 (MQ=255)                                                                                          ccTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGATTTTTATTGTGTTGATGGGTTAGGAATTGATGGACAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATc                                                                                                                         >  1:144297/1‑251 (MQ=255)                                                                                                     cACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGAGCTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGAGTGGGCTAAGTACCCGTCACATACg                                                                                                                                                                                                                                       >  1:195372/1‑131 (MQ=255)                                                                                                                       ttGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCTGCAGACGCCATCAAAATGGCCGGTCCGATCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTgn                                                                                             <  1:39180/250‑2 (MQ=255)                                                                                                                         gTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGGTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCa                                                                                                                                                                        <  1:204834/173‑1 (MQ=255)                                                                                                                                        ctgtgATGCCCCGTGGTTTTTTATTGTGTTGATGGGTAAGAAATTGATGGAACGTAAGAACAAGACAAAACGAGGGCTAAAAACCAGTCACATACGCATTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCCGCCGCCATCAAAATGACCGGTCCAAGCGTGTTGTTAGACTATATTATCGGTGGAATCGCGGCGTATATCATTATGCGAGCGCTGGGGGCAAAGTCGGTACAt                                                                            >  1:230107/5‑250 (MQ=255)                                                                                                                                               gCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCggn                                                                    <  1:211897/251‑2 (MQ=255)                                                                                                                                                ccccGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGgcn                                                                   <  1:86260/251‑2 (MQ=255)                                                                                                                                                                 gtgtTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTGTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCTGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATGATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCgg                                                                                 <  1:155861/220‑1 (MQ=255)                                                                                                                                                                                      aTGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGta                                                                                                                 >  1:7052/1‑167 (MQ=255)                                                                                                                                                                                        ggAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGcc                           <  1:10091/251‑1 (MQ=255)                                                                                                                                                                                                      aGCTAAAGCGTGGGCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTGT‑ACGGTTCGGCAGACGCCAGCAAAATgg                                                                                                                                                                  <  1:83501/102‑1 (MQ=255)                                                                                                                                                                                                                 gggCTAAGTCCCCGTCACATACGCTTTATTGCACTGGGTTCAGCAATTGGCCCCGGGCTGTTTTTACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTTGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGTTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGTTTACATTACCGGc    <  1:255998/250‑1 (MQ=255)                                                                                                                                                                                                                 gggCTAAGTACCCGTCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACAGAGCCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGATAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTg  <  1:124628/251‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                              GGCCGAACAAGGTCTGGCGTGGTTAATGCCAACAGTGGTGATGGTGGTTCTGGCCATTATCTGGGATCGCGCGGCAGGTCGTCAGGTGACCTCCAGCGCTCACTAAATCACTGAACATTTGTTTTAACCACGGGGCTGCGATGCCCCGTGGTTTTTTATTGTGTTGATGGGTTAGGAATTGATGGAAAGTAAGAACAAGCTAAAGCGTGGGCTAAGTACCCGCCACATACGCTTTATGGCACTGGGTTCAGCAATTGGCACCGGGCTGTTTT‑ACGGTTCGGCAGACGCCATCAAAATGGCCGGTCCGAGCGTGTTGTTGGCCTATATTATCGGTGGTATCGCGGCGTATATCATTATGCGTGCGCTGGGGGAAATGTCGGTACATAACCCGGCCGCCAGCTCTTTCTCGCGTTATGCGCAGGAAAACCTCGGCCCGCTGGCAGGTTACATTACCGGCTG  >  NC_000913/420805‑421263

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑