Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	420,423	G→A	G278D (GGC→GAC)	brnQ →	branched chain amino acid transporter BrnQ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	420,423	0	G	A	100.0%	23.0 / NA	11	G278D (GGC→GAC)	brnQ	branched chain amino acid transporter BrnQ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (6/5);  total (6/5)

CCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTTCTCGATGGTGGTTTCTAACCTCGGCTTAAGCCAGCTGATCCAGATCTCCGTACCGGTGCTGACCGCTATT  >  NC_000913/420246‑420631                                                                                                                                                                                  |                                                                                                                                                                                                                 ccGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCGGGGGTCAGACAGCGCGGCGCTGGTCGATCAGTCTGCAAACGTCGCTGCTATTCTGCATGCTTATGTTCAGCACACCTTTGGCGGCGACGGTAGCTTCCTGCTggc                                                                                                                                                                                                <  1:234784/196‑1 (MQ=255)          gTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGTCTGTCGTGCAACTGGCCCTGTTCCGTCTGAGGCCAGACAGGGCGTCGATGGTAGCTCCGTCTGCCAACGGCGCTGCTTTTCTGCATGCTTACGTCCCGCCCACCTTTGGCGGCGACGGGAGGTTCCTGCTGGCGGCGTTCATCTTCATCGCCTGCCTGGTAACGCAAGTTGGCCTCACCTGTGCTTGTGAAGAATTc                                                                                                                                >  1:173869/1‑251 (MQ=255)                             cGTCTGGGCTGGCCTGATGGCGGGTGTTGGGCTGACTCTGCTGGACCTGGCGCTGTTCCGGCTGGGGTCAGACCGGGCGTCGCTGGTCGATGAGTATGCCAACGGCGCTCCTCTTTTGCATGCTTACGTTCAGAACACCTTTGGCGGCGACGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGGGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGc                                                                                                             >  1:359304/1‑251 (MQ=255)                                                                                                 cAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCGGCTATTCTGCATGCTGACGTTCAGCACACCTTTGGCGGCGACGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTTCTCGATGGTGGTTTCTAACCTCGGCTTAAGc                                         <  1:330122/251‑1 (MQ=255)                                                                                                       nngcnNNNNTGGNCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGACGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCt                                                                                                                                            <  2:221629/144‑1 (MQ=255)                                                                                                              gCTGGTCGNTNNNTNNGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTNGGCGGCGACGGTAGCNTCNTGCTNGCGNCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCCGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTTNCCTNGGCGGNNTNTCGATgnnngtnnnnnnnncnnnnccnnann                                        >  2:242643/1‑219 (MQ=255)                                                                                                              gCTGGTCGATCAGTCTGCCAACGGCGCTGCTATTCTGCATGCTTACGTTCCGCACGCCTTTGGCGGCGACGGTAGGTTCCTGCTGGCGGCGTTACTCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGACTTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTTCTCGATGGTGGTTTCTAACCTCGGCTTAAGCCAGCTGATcc                               >  1:267845/1‑248 (MQ=255)                                                                                                              gCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGACGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTTCTCGATGGTGGTTTCTACCCTc                                                  <  1:242643/229‑1 (MQ=255)                                                                                                                                        ctgctATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGACGGTAGCTTCCTGCTGGCGGCGTTAATCTTAATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGAACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTTCTCGATGGGGGTTTCTAACCTCGGCTTAAGCCAGCTGATCCAGATCTCCGTACCGGGGATGACCGCTAtt  >  1:312481/1‑251 (MQ=255)                                                                                                                                                 tGCATGCTTACGTTCAGCACACCTTTGGCGGCGACGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTGCTCGATTGTGGTTTCTAAc                                                     <  1:299616/191‑1 (MQ=255)                                                                                                                                                       cTTACGTTCAGCACACCTTTGGCGGCGACGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGAAACGGCAGTTGGCCTGACACGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTTCTCGATGGTGGTTTCTAACCTCGGCTTAAGCCAGCTGATc                                >  1:204633/1‑206 (MQ=255)                                                                                                                                                                                  |                                                                                                                                                                                                                 CCGAAGCGCGTCTGCTGACCCGTTATACCGTCTGGGCTGGCCTGATGGCGGGTGTTGGTCTGACTCTGCTGTACCTGGCGCTGTTCCGTCTGGGGTCAGACAGCGCGTCGCTGGTCGATCAGTCTGCAAACGGCGCTGCTATTCTGCATGCTTACGTTCAGCACACCTTTGGCGGCGGCGGTAGCTTCCTGCTGGCGGCGTTAATCTTCATCGCCTGCCTGGTAACGGCAGTTGGCCTGACCTGTGCTTGTGCAGAATTCTTTGCCCAGTACGTACCGCTCTCTTATCGTACGCTGGTGTTTATCCTCGGCGGCTTCTCGATGGTGGTTTCTAACCTCGGCTTAAGCCAGCTGATCCAGATCTCCGTACCGGTGCTGACCGCTATT  >  NC_000913/420246‑420631

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑