Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,510,238	T→G	intergenic (+445/+452)	insO → / ← fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,510,238	0	T	G	100.0%	60.8 / NA	22	intergenic (+445/+452)	insO/fecE	IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (9/13);  total (9/13)

CTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTG  >  NC_000913/4510000‑4510429                                                                                                                                                                                                                                               |                                                                                                                                                                                                cTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGt                                                                                                                                                                                              <  1:184630/242‑1 (MQ=255)                                          ccTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGccn                                                                                                                                            <  1:64437/251‑2 (MQ=255)                                            ttCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAACTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGcc                                                                                                                                             <  1:266567/248‑1 (MQ=255)                                                cttgaTGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTAATTAAGAAAGCTGCACCTACCTAATTAGGAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAAGTCCGTTCCAGTGTTGCTTTTGATTTACGTAGGCGTCTGATCATTGATTTAGCCTCAAAAGcg                                                                                                                                             <  1:45251/240‑2 (MQ=255)                                                               ggTAAGCTGGGTGAGACACCGACTCATGAAGATAGCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGGAGTAAACGCACACTGTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGTGTTGCTTTTGATTTACGTTTGCGTCTGATCATGTATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCa                                                                                                                       <  1:345625/251‑1 (MQ=255)                                                                                       cATGAAGATATCAGTGATAACAATCGATCCATCAACTGGCCTGTATATCGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTATAGAAAAATCGATGGTCAGAAACTGGAGTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCGCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGc                                                                                                <  1:251605/250‑1 (MQ=255)                                                                                                   aTTGATAACAATCGATCCATCAACTGGCCTGTATAACGAGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCAAGGGTTGCTTTTGATTTACGTTGGGGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAAtt                                                                                   >  1:149472/1‑251 (MQ=255)                                                                                                                                  nnnnnngTNNNNNCCGNTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTa                                                                                                                                                         <  2:209108/144‑1 (MQ=255)                                                                                                                                   ataACGTGGTTGCTGGTTATTAATAAATCTGCACCTACCTAAGTAGTAGCAAACGCAAACTTTTTATAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGTTTTGCTTTTTATTTACTTTTGCGTCTGATCATTTATTTATCCTCAAAAGCCCAACCTCATTGGGAATGATCCAGCTCCGTGAATGTCCTCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAg                                                    <  1:252633/250‑1 (MQ=255)                                                                                                                                           ncTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTg                                                                                                                                                                            >  1:101700/2‑122 (MQ=255)                                                                                                                                             tGCCGGGGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTCATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGt                                         >  1:79/1‑251 (MQ=255)                                                                                                                                                    gATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTTAGTGACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTTATGAACCAGCTCCGTGAATGTCCGCTCTGGCACagag                                                                                          <  1:332192/195‑1 (MQ=255)                                                                                                                                                          gAAAGCTGCACCTACCTAAGTTGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGACACTGGATTAGAAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAtt                                                                                                                                                           >  1:236965/1‑124 (MQ=255)                                                                                                                                                           aaatcTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGCTCAGAAACTGGATTAGAAATTCCGTTCCAGGGTTGCTTTTGCTTTACGTTGGCGTCTGATCATTGATTTATCCTCACAAGCCCAACCTCATTGGTCATGAACCCGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGCTCTCCCCCCTGCAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTa                            >  1:49487/5‑250 (MQ=255)                                                                                                                                                                      tacctaAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGcac                                                                                              <  1:270228/173‑1 (MQ=255)                                                                                                                                                                                    cAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTg  >  1:145110/1‑251 (MQ=255)                                                                                                                                                                                         gCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGcn                                                                                        <  1:84986/160‑2 (MQ=255)                                                                                                                                                                                                                   cAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                                                                                                                                            <  1:16811/66‑1 (MQ=255)                                                                                                                                                                                                                   cAGAAACTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGAt                                                                                                                                                            >  2:16811/1‑66 (MQ=255)                                                                                                                                                                                                                        aCTGGATTAGCAATTCCGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAAcc                                                                                                                                        >  1:108853/1‑81 (MQ=255)                                                                                                                                                                                                                                   aaTTCCGTTCCAGGGGTGCTTTTGATTTACGTTGGAGTCTTATCATTGATTTATCCTCAAAAGCCCAACATCATTGGTAATGAACCAGCTCCGTGAATGTACGCTCTGGCACAGAGCGAAATTTTTTGATCTCGCCCCTGaaa                                                               <  1:828/143‑1 (MQ=255)                                                                                                                                                                                                                                        cGTTCCAGGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCCGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGtttt                                      >  1:163901/1‑163 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                CTTTCCCTCTGGCGAAAAAGGGAGAAGGACGGGCCCGTCTGCCTTCACTTTATGCCAGCTTCGGTAAGCTGGGTGAGACACCGACTCATGAAGATATCATTGATAACAATCGATCCATCAACTGGCCTGTATAACGTGGCTGCCGGTGATTAAGAAAGCTGCACCTACCTAAGTAGTAGCAAACGCACACTTTTTAGAAAAATCGATGGTCAGAAACTGGATTAGCAATTCCGTTCCATGGTTGCTTTTGATTTACGTTGGCGTCTGATCATTGATTTATCCTCAAAAGCCCAACCTCATTGGTAATGAACCAGCTCCGTGAATGTCCGCTCTGGCACAGAGCGAAATTTTTTGATCTCCCCCCTGAAATCTAAACTTAGTCATGTCACGTTTTTGGGTTTCTAAAATTTTAACTTCGCGTTTTTCGTTG  >  NC_000913/4510000‑4510429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑