Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,499,998	G→C	intergenic (+498/‑1262)	insD6 → / → yjgZ	IS2 insertion element protein InsB/uncharacterized protein YjgZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,499,998	0	G	C	100.0%	26.4 / NA	11	intergenic (+498/‑1262)	insD6/yjgZ	IS2 insertion element protein InsB/uncharacterized protein YjgZ
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (5/6);  total (5/6)

GCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGCGAAGCTGCCTTTCTTGAGGAAAGT  >  NC_000913/4499768‑4500201                                                                                                                                                                                                                                       |                                                                                                                                                                                                            gCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGAGGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGGAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGCTAACAgn                                                                                                                                                                                                      <  1:37670/238‑2 (MQ=255)            tCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTTGCACACTGGAGCCTCCCCGCACATTACCATAATGATGAACAGGAATGGCAATGTCGCTGACCGAAAGCCCAAGGCCAGAAAAATAAAACATGTCGAAATGACGTGCGTACCGGCGAAGGATACCATCAAGCCGGCCAAGAAAGTTATCCCGGTTAATGATGCTGGAGAGCTAACAGGCAATTTTTCGGGGATACTGCTcc                                                                                                                                                                               >  1:8015/1‑250 (MQ=255)                          gatgaGACGTTATGGCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATAGCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCGTTCGAGCTAACAGGCAATTGTTCGTG‑ATACTGCCCCAGGTAATTATTCGGc                                                                                                                                                                <  1:318701/250‑1 (MQ=255)                                                ggTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGTTACCATCAAGCTGGCCAAGAAAGGTATCCAGTTTACTGATGCTGGCGAGCTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTAGGCGGCTAGGAGTTAAGGCt                                                                                                                                                  <  1:127161/243‑1 (MQ=255)                                                                                                                     gCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGCAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTCACTGATGCTGGCGAGCTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGCGAAcc                                                                                                                          >  1:334091/1‑198 (MQ=255)                                                                                                                      cTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGCTAACAGGCACTTTTTCGGGGATACTGCTCCAGGTAATTATTCGGCTAg                                                                                                                                                             >  1:56731/1‑162 (MQ=255)                                                                                                                                          aGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGCTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCg                                                                                                                                                                  >  1:139588/1‑137 (MQ=255)                                                                                                                                                                 gACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGCTAACAGGCAATTTGTCGGGGATACTGCTCCAGGTAATTATTCGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGgaagc                          <  1:106769/250‑1 (MQ=255)                                                                                                                                                                                           tCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGCTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGCGAAGCTGCCTTTCTTGAGGAAAGt  <  1:119030/248‑1 (MQ=255)                                                                                                                                                                                                   gCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGCTAACAGGCAATTTTTGAGGGATACTGCTGCTGGTACTTATTCGGCTAGGAGTTACGGCTGTCACACGGATTTGGATGAGAACCCATCCTGTGCAGGGATATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGt                                      >  1:352458/1‑204 (MQ=255)                                                                                                                                                                                                                      tnNCTGATGNNGGCGAGCTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGaa                                                                                                                            <  2:8015/99‑1 (MQ=255)                                                                                                                                                                                                                                       |                                                                                                                                                                                                            GCACTGATTTTTCTACTGACGGGCTGATGAGACGTTATGTCACTGGCGGTAATAATCAGTGGCACACTGTAGCCTCCCTGCACATGACCATAATGATGAACAGGATTGGCACTGTCGCTGACCGACAGCCCAAGGCCAGAAAAGTAAAGCATGGCGAAATGACGTGAGTACCGGCGAAGGATACCATCAAGCTGGCCAAGAAAGTTATCCAGTTTACTGATGCTGGCGAGGTAACAGGCAATTTTTCGGGGATACTGCTCCAGGTAATTATTCGGCTAGGAGTTAAGGCTGTCACACGGATTTGGATGAGAACCCATCATGTGCAGGAAAATTATCTTCGGAGAGGATGTATCCGCCAGCGCACGTTCTGTTTCCTGTAACAACAGTTTGTCATCTGTTTTACGGGAAGCGAAGCTGCCTTTCTTGAGGAAAGT  >  NC_000913/4499768‑4500201

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑