| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,489,016 | C→T | E350K (GAA→AAA) | yjgR ← | DUF853 domain‑containing protein YjgR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,489,016 | 0 | C | T | 93.3% | 36.3 / NA | 15 | E350K (GAA→AAA) | yjgR | DUF853 domain‑containing protein YjgR |
| Reads supporting (aligned to +/- strand): ref base C (0/0); major base T (7/7); minor base G (0/1); total (7/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.63e-01 | |||||||||||
GAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTCGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGC > NC_000913/4488788‑4489241 | gAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTgn < 1:102115/249‑2 (MQ=255) ccTTTTTGACACCTCTCATAGGCGGGTTCCCGGTAAACCTCATCCTCATATTTGCACTACACCGGAGAGTGATTACTCAAGCCATTAGGCTCATCTTCCGTCACCGGCCCTATCCGCGCACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAACAGAGATCAACGCCTCGCCGGTTCCCAGTTCCTGCATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCAt > 1:295785/1‑251 (MQ=255) tttttGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTAGTATCAAACGCCTGATTGGCCCGCATg < 1:77740/250‑1 (MQ=255) cACCTCATCCTCATATTTGCCATACCCCGGAGAGCGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCACCATCCGCGAACAAGGCGAGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCCCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCtt > 1:170870/1‑251 (MQ=255) cctcatcctcatATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGTCGCGATCACCATCGAAAGCTCCACCACAGAGGGGCTTCCTTTCGCATACAGAAAAGAGATCAGCGCCTCGCCGGTGCCCAGTTCCTGAATCGACTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCtttt > 1:244148/1‑251 (MQ=255) atcctcatATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGTCGGTGCCCAGTTCCTGAAGCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCg < 1:237973/251‑1 (MQ=255) ncGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCAGTCACCGGCCCCATCCGCGAACAAGGAGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCAGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGCTCTTTTGGCGtcaa > 1:37307/2‑232 (MQ=255) ggCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCTGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCGTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCg < 1:141543/244‑1 (MQ=255) gAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTg > 1:281589/1‑251 (MQ=255) gcgATCACCATCGCACGCTCCACCACAGTAGGCCTTCGTTTCGGATCTACAAAAGAGATCAACTCCTCGCCCGTGCCCATTTCCTGAATCGCGTTAGGGGTATCAAACGCCGGATTGGCCCGCATGGTTTgcg < 1:237217/133‑1 (MQ=255) gCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGGAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAg < 1:158321/241‑1 (MQ=255) gAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTTGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAATCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCTAGCTCATTATCCGGAATATCAGTCGGGTTTTGCGAAACGAACCAGACGCCCACTCCTTTTGAGCGAATAAGCCTTATCCCCTGc < 1:342354/245‑1 (MQ=255) nganaTCAANGCCTNGCCGGTGCCCAGTTCNTGAAGCGCCTTTTTGGTACNNANCGCCGNATTgg < 2:153762/64‑1 (MQ=255) gTGCCCAGTTCCTGATTCGCCTTTTTGGTATCGATCGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGc > 1:119936/1‑251 (MQ=255) gCCTTTTTGGTATCAAACGCAGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAACGCATGTTGAACGCGATTACCGCGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGcc > 1:290464/1‑193 (MQ=255) | GAAAGCCTTTTTGCAACATCTCATAGGCGGATTCCCGGTCCACCTCATCCTCATATTTGCCATACACCGGAGAGTGATTAATCAAGCCATTACGCTCATCTTCCGTCACCGGCCCCATCCGCGAACAAGGCGCGATCACCATCGCACGCTCCACCACAGAAGGGCTTCCTTTCGCATCCAGAAAAGAGATCAACGCCTCGCCGGTGCCCAGTTCCTGAATCGCCTTTTCGGTATCAAACGCCGGATTGGCCCGCATGGTTTGCGCCGCTGCCTTCACTGCTTTCTGATCTTTTGGCGTAAAAGCCCGCAAAGCATGTTGAACGCGATTACCGAGCTGCCCGAGCACATTATCCGGAATATCAGACGGGTTTTGCGAAACGAACCAGACGCCCACGCCTTTTGAGCGAATAAGCCTTATCACCTGCTCAATCTTATCCAGCAGTACCTGCGGGGC > NC_000913/4488788‑4489241 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |