Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,479,944	G→A	G72E (GGG→GAG)	yjgN →	inner membrane protein YjgN

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,479,944	0	G	A	100.0%	63.0 / NA	22	G72E (GGG→GAG)	yjgN	inner membrane protein YjgN
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/12);  total (10/12)

AATGGATTTTTTAATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGG  >  NC_000913/4479702‑4480189                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      aaTGGATTTTTTAATTCAGGGAATTTTTATGGCTCAAGATCTTAATGAACTGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGAGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTAACATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTATTATGGAATTACCGGTGAGAATGttt                                                                                                                                                                                                                                               >  1:158232/1‑251 (MQ=255)            tAATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTg                                                                                                                                                                                                                                     <  1:147860/250‑1 (MQ=255)                                     aGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTTCCATGGGCATTAATGAAATGTAAGCGTGATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTcn                                                                                                                                                                                                           <  1:69701/251‑2 (MQ=255)                                                           ccGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTcagc                                                                                                                                                                                     <  1:54044/251‑1 (MQ=255)                                                               nccATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTTGTTGTCTTTTTTTTGTTTTTTTTTATTTCGc                                                                                                                                                                                                          >  1:210737/2‑226 (MQ=255)                                                                          gtttTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGc                                                                                                                                                                                                          >  1:25907/1‑215 (MQ=255)                                                                                         ggTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTGTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGAc                                                                                                                                                       <  1:337361/251‑1 (MQ=255)                                                                                            nagagaTATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATATTTATGCTAATATGGAAGCTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTcagcag                                                                                                                                                                                   >  1:37826/2‑220 (MQ=255)                                                                                                               gtgtgGGGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTCAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGACTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCtt                                                                                                                                 >  1:181119/1‑251 (MQ=255)                                                                                                                          gtgtTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATgg                                                                                                                       <  1:170577/250‑1 (MQ=255)                                                                                                                                  aaCGATTATAACGCTAGGTATCTATTTACCATGGGCATTACTGAAATGTAAGCGTTCTATTTATGCTAATATGGAAGTTAACGGAAAACGATTTTCTTATGGAATTACCGGTGAGACTGTTTTTGTTAGTTGt                                                                                                                                                                                                                                    >  1:340278/1‑133 (MQ=255)                                                                                                                                  aaCGATTATAACGCTAGGTATCTAGTTACCATGGGCATGAATGAAATGTAATCGTTATCTTTATGCTAATATGGAAGTTAACGTACAACGATTTGCTTATGGAATTACCGGTGAAAATGTTTTTGTTAGTTg                                                                                                                                                                                                                                     <  1:234467/132‑1 (MQ=255)                                                                                                                                       nnnnnnnnnnaGNNNNNTATNTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGc                                                                                                                                                                                                          <  2:210737/144‑1 (MQ=255)                                                                                                                                                                     cATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGACATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAAc                                                                           >  1:348784/1‑251 (MQ=255)                                                                                                                                                                                              tnNTGCTAANNTGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGc                                                                                                                                                                                                          <  2:25907/99‑1 (MQ=255)                                                                                                                                                                                                       tatGGAAGTTAACGGACAACGAGTTTCTTATGTAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCaa                                                                                                                                                                                                        <  1:199250/92‑1 (MQ=255)                                                                                                                                                                                                          ggAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCACCGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGt                                      >  1:280128/1‑251 (MQ=255)                                                                                                                                                                                                           gAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTc                                                                                                                                                                                                                  <  1:113549/78‑1 (MQ=255)                                                                                                                                                                                                           gAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATCTGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGc                                                                                              >  1:252346/1‑194 (MQ=255)                                                                                                                                                                                                                           gATTTTCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGGTCTGGTGGGGGACCttt                     >  1:13612/1‑251 (MQ=255)                                                                                                                                                                                                                              tttCTTATGGAATTACCGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCttttt                   <  1:177890/250‑1 (MQ=255)                                                                                                                                                                                                                                              cGGTGAGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTTTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTGATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGAATTAGTGTTAATGGCTCTATGAAAGTGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATgg  <  1:312971/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      AATGGATTTTTTAATTCAGGGAATTTTTATGGCTCAAGTTATTAATGAAATGGATGTTCCGTCCCATTCGTTTGTTTTTCATGGTACAGGTGAGAGATATTTTCTTATTTGTGTGGTGAATGTGTTGTTAACGATTATAACGCTAGGTATCTATTTACCATGGGCATTAATGAAATGTAAGCGTTATCTTTATGCTAATATGGAAGTTAACGGACAACGATTTTCTTATGGAATTACCGGTGGGAATGTTTTTGTTAGTTGTCTTTTTTTTGTTTTTTTCTATTTCGCAATCTTAATGACAGTGTCAGCAGATATGCCGCTTGTTGGTTGTGTTTTGACTTTGTTACTGTTGGTTTTGCTTATATTTATGGCAGCAAAAGGACTGCGTCATCAGGCCTTAATGACCAGTCTCAACGGCGTAAGATTTAGTTTTAATTGCTCTATGAAAGGGTTCTGGTGGGTGACCTTTTTCTTGCCGATTTTAATGG  >  NC_000913/4479702‑4480189

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑