Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,476,605	G→A	S390N (AGC→AAC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,476,605	0	G	A	100.0%	40.3 / NA	15	S390N (AGC→AAC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/5);  total (10/5)

GATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGG  >  NC_000913/4476388‑4476775                                                                                                                                                                                                                          |                                                                                                                                                                           gATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATaa                                                                                                                                            >  1:196069/1‑250 (MQ=255)                         cACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATGAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATAtt                                                                                                                                                                 <  1:332024/205‑1 (MQ=255)                                     aaTGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGCTTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTACTAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCg                                                                                                                             >  1:190554/1‑229 (MQ=255)                                                   tAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCACAGGTTAACCTTACAACGGTCATCGATTCGATTCTCGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATg                                                                                                                                                                        >  1:310472/1‑172 (MQ=255)                                                                     cATCAAAGTTACCCTGTCTCATATTATTATTGATTCCTTAGATGATGCACAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTACGACTACTGGATTGGTGTTTTAATAACAACATGCAATATTTTGATGATACTAAACAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTCAAATAGCTGAGACattat                                                                       >  1:105465/1‑251 (MQ=255)                                                                     cATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACattat                                                                       >  1:154259/1‑251 (MQ=255)                                                                                                        ncTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTAttttt                                                                   >  1:219620/2‑220 (MQ=255)                                                                                                                                      cGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATa        >  1:81813/1‑249 (MQ=255)                                                                                                                                                            gaCTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAgttgg  >  1:116216/1‑233 (MQ=255)                                                                                                                                                              cTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGATACAGTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAgg                       <  1:227244/210‑1 (MQ=255)                                                                                                                                                                ttttCNNAANNNCCNNATATTAATGATGTAAGAATACTGNATTGGTGTTTTAATAAAAACATGCAATATNTNGNTGATNNTAANACGATAAAGCNTGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAANANCGCTGAGACATTATTTTTCAATCTCg                                                           >  2:266769/1‑172 (MQ=255)                                                                                                                                                                ttttCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAAGCTTCGCATCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCg                                                           <  1:266769/172‑1 (MQ=255)                                                                                                                                                                        aTCCGCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCa                                                                                              >  1:10737/1‑129 (MQ=255)                                                                                                                                                                          nnnnnnnnnntANNNNNGTANGAATACTGGATTGGTGTTTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTAttttt                                                                   <  2:219620/144‑1 (MQ=255)                                                                                                                                                                                                      gnNTTGGTGNNTTAATAAAAACATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCa                                                                                              <  2:10737/99‑1 (MQ=255)                                                                                                                                                                                                                          |                                                                                                                                                                           GATAAGCTCTACGCATTAAATGATCACGGAATTGATAATGACAAAGTAGGTAACAATGAAATTAATGACATCAAAGTTAACCTGTCTCATATTCTTATTGATTCCTTAGATGATGCAAAGGTTAACCTTACACCGGTCATCGATTCGATTCTGGAGACTTTTTCAAAATCCCCATATATTAATGATGTAAGAATACTGGATTGGTGTTTTAATAAAAGCATGCAATATTTTGATGATACTAAAAAGATAAAGCATGCATGCTCCGTAATAAATCATATTAATCTTCGCAGCGATCAGTCTAAAATAGCTGAGACATTATTTTTCAATCTCGATAAAGAACCCTATAAAAATAGCCCTGAATTACAGGGGTTGATTTGGAATAAGTTGG  >  NC_000913/4476388‑4476775

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑