Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,475,493	T→C	F19F (TTT→TTC)	yjgL →	protein YjgL

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,475,493	0	T	C	100.0%	78.0 / NA	21	F19F (TTT→TTC)	yjgL	protein YjgL
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/12);  total (9/12)

CCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCT  >  NC_000913/4475270‑4475698                                                                                                                                                                                                                                |                                                                                                                                                                                                               ccAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTAAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATAcc                                                                                                                                                                                     <  1:278886/251‑1 (MQ=255)               cAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAAt                                                                                                                                                                                        >  1:58134/1‑234 (MQ=255)                aaaGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTAGTCTCAACACATTACAGTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGgaatg                                                                                                                                                                      <  1:177752/251‑1 (MQ=255)                           gAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGc                                                                                                                                                           <  1:75270/251‑1 (MQ=255)                           gAAGATGCTGATGGCGTGATGACGTTTCGCCGTAAATAACTCAAGGTTGACGGCGAGTTTGTGAAAAGAGCGTTTATTAATATTTTTT‑‑TGAGTAAAATTTGTGATACTTATACGTGCGTATTCACTCAAGGAGTTCGTCATGATCAAAATATCAGATTTAAATTATTCTCAACACATTACATGAGCTGACAATTTCAAACAATAAAGTGAATTTTTAAATACCTTTCGTGTTGGAATGAATGATTTTGccc                                                                                                                                                         <  1:344956/251‑1 (MQ=255)                                               gaCTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAAGTATTCTCAACACATGACATTAGCCGACAATTTCAAACAAAAAAATGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAg                                                                                                                                       <  1:264270/250‑1 (MQ=255)                                                       gCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAAc                                                                                                                                 >  1:230181/1‑248 (MQ=255)                                                                     aaGGTTTACGGCGATTTTGTGAAAAGATCGTTTTTTGATATTTTTTTGTGTGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATTAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTCGCCAACAATTTCAAACAAAAAAGTGAAGttt                                                                                                                                                                                             <  1:288905/175‑1 (MQ=255)                                                                                                        tGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGata                                                                              <  1:206198/250‑1 (MQ=255)                                                                                                         natatTTTTTTGTGAGTAAAATTTGTAATGCTTAGACCTTCTTATTCACTCACGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACCCATTACATTAGACGACAATTTCAAAAAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATAtt                                                                            >  1:35090/2‑251 (MQ=255)                                                                                                                                                           cAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAg                                                                                                                               <  1:126367/150‑1 (MQ=255)                                                                                                                                                                 gTTCGTCATTAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGt                                                                  >  1:7774/1‑205 (MQ=255)                                                                                                                                                                 gTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑NAGGNNNNNAgnnnnnn                                                                                                                         >  2:217313/1‑144 (MQ=255)                                                                                                                                                                 gTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAgn                                                   <  1:217313/220‑2 (MQ=255)                                                                                                                                                                     gTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGGGCAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATgg                 >  1:1583/1‑251 (MQ=255)                                                                                                                                                                     gTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAg                                                    >  1:224168/1‑215 (MQ=255)                                                                                                                                                                             cAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCggg                                                                                                                                              <  1:266487/118‑1 (MQ=255)                                                                                                                                                                             cAAAANNTCNNNTTNNAATTATTCTCAACACATTACATTNGCCGACAATTTCAAACAAAAAAGTGAAGTNTNANATACNNGGCNTGTTGGAATGNATGATTTTGCCCGTATTGCCggg                                                                                                                                              >  2:266487/1‑118 (MQ=255)                                                                                                                                                                                   atCAGATTTAAATTATTCTCAACACATTACATTAGCCAACAATTTCAAACAAAAAAAAGAAGTTTTAAATACCTGGAGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAGAAGGAATCTTCTTTCTCCTGAAGAATTTTTAGAGTTTTTGGCAAAGATATTTCCCCTGGGTTATGTGGATTTTAGCAAACGCTCAAACGAAGCGGGAAGAAATATGATGGCTCATATTAAGTctt  >  1:182067/1‑249 (MQ=255)                                                                                                                                                                                               ttattCTCAACACATTACATTAGCCGACAATTTCAAACAAAAAAGTGAAGGGTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCa                                                                                                      <  1:354833/139‑1 (MQ=255)                                                                                                                                                                                                                       cGACAATTTCAAACAAAAAAAAGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGGATTGCCGTGTGG‑CATGATAACAGAAGGTATAGTCTGTCTCCTGGAGCATTTTTAGAGTTTTGGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAAc                                               <  1:345794/170‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                               CCAGCCCAGGTTCGCAAAGCAGTAGTGAAGATGCTGATGGCGTGATGACTTTTCGCCGTAAATAACTCAAGGTTTACGGCGAGTTTGTGAAAAGAGCGTTTTTTGATATTTTTTTGTGAGTAAAATTTGTAATGCTTAGACGTTCTTATTCACTCAAGGAGTTCGTCATGAGCAAAATATCAGATTTAAATTATTCTCAACACATTACATTAGCCGACAATTTTAAACAAAAAAGTGAAGTTTTAAATACCTGGCGTGTTGGAATGAATGATTTTGCCCGTATTGCCGGGGGG‑CAGGATAACAGAAGGAATATTCTTTCTCCTGGAGCATTTTTAGAGTTTTTGGCAAAGATATTTACCCTGGGTTATGTGGATTTTAGCAAACGCTCCAACGAAGCGGGTAGAAATATGATGGCTCATATTAAGTCCT  >  NC_000913/4475270‑4475698

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑