Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,437,890	C→T	Q62* (CAG→TAG)	ytfI →	protein YtfI

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,437,890	0	C	T	100.0%	17.6 / NA	8	Q62* (CAG→TAG)	ytfI	protein YtfI
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (3/5);  total (3/5)

TTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA  >  NC_000913/4437665‑4438102                                                                                                                                                                                                                                  |                                                                                                                                                                                                                     ttAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATTTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCTTATACAAAAACACATGCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAATAGATTGGATTTCGAATTATGATAGCCTGTGAACGTTGAGGGAGTGCTTTCGTTGCGCAACTTATATTGATAAAAGTGGTTATCAgg                                                                                                                                                                                              <  1:325566/250‑1 (MQ=255)        cGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTCTACAAAAACCCATTAAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTtc                                                                                                                                                                                       >  1:260117/1‑250 (MQ=255)                           aaaataaGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTc                                                                                                                                                                   <  1:130494/251‑1 (MQ=255)                                                                                                                          cAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCATCCGTAAAGATAACGGTGCCGAATATACAATGACATTGCGAgn                                                                                          <  1:69833/229‑2 (MQ=255)                                                                                                                                                                         tGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGCTAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTAaaaa                      >  1:5777/1‑250 (MQ=255)                                                                                                                                                                                                     cTTTGAGGGAGTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCaaaa                                                                                                                                                                                >  1:145384/1‑68 (MQ=255)                                                                                                                                                                                                               gTTCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTTCTTTCATCAAAAAATGAAACTGTTCACACCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTATTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGaa  <  1:316826/232‑1 (MQ=255)                                                                                                                                                                                                                 tCTTTCGTTGCGCAACTTAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATgn                               <  1:103953/201‑2 (MQ=255)                                                                                                                                                                                                                                  |                                                                                                                                                                                                                     TTAATGGCGACATAAAGTAACCAAATAAAATAAGGTTGTCATATGTTACCCAGGATCAGACACAATAATTTTATTGGTGCGGTGGAGTTATTTGTAAAGTCTTCGTATACAAAAACACATTCAAACAATTTTTTTAACAACATCCATCATGCATTTAAGAAAAAAGATTGGATTTCGAATTATGATAGCCTGTTAACTTTGAGGGAGTTCTTTCGTTGCGCAACTCAGATTGATAAAAGTGGTTATCAGGTGCTTTCATCAAAAAATGAAACTGTTCACGCCATGGATAAGTTTCTTATCAGTTTCAGCCTTAAAGATAACGGTGCCGAATATACAATGACATTGCGAGGTAGTGGATTTGAATACGAAGAAATACCCATAACAATAAATGAATATAACTCATTCATGGATTTCAAAAACCGTGAATTTCCGTTAGAA  >  NC_000913/4437665‑4438102

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑