Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,331,890	C→T	L463L (CTC→CTT)	proP →	osmolyte:H(+) symporter ProP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,331,890	0	C	T	100.0%	35.9 / NA	15	L463L (CTC→CTT)	proP	osmolyte:H(+) symporter ProP
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/8);  total (7/8)

CATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATA‑TTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGG  >  NC_000913/4331676‑4332107                                                                                                                                                                                                                       |                                                                                                                                                                                                                           cATTTAAGATTTCGGTGCTGGTTGCCGTTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTTCCTGATGGTAGTGGCGGTGGGTGGTTTAATCTCCGGCGTAACCATGAAATAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGTCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAg                                                                                                                                                                                                                      <  1:147879/221‑1 (MQ=255)                                       aCGCTGGCGGCCTGGCTGGTCGAAAGCGCGCAGAAGCTGATGATGCCTGCCTATGTCCTGATGGTAGGGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATA‑TTGACCACGAGATTGCCGATTTGCAGGCGAAAcn                                                                                                                                                 <  1:67823/251‑2 (MQ=255)                                                            gAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGAGGTTGGGTTAATTACCGGCGCAACAATGAAAAAGACGGCAAAAAGTCCGGTGAAAGGGGCGACACCGGCGGCGGCAGATATAAAGGAAGGGGAGGAAAATCTTGTCGGGCATTAACATAAAATTGAGCAGGAAAAAGAAGATAATTAACCA‑GAGATTGCCGATTTTCAGGCGagatcgggaaaggaaccacctgaac                                                                                                                             >  1:100682/1‑226 (MQ=255)                                                                                               ccggATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACCCCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGTATTACGATAATATCGAGCAAAAAATCGATGATA‑TTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCTAATTGATGAATAAGCTGAAACGGATGGcc                                                                                         <  1:294183/248‑1 (MQ=255)                                                                                                                 ggttggttTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGCAAGGTGAGACACCGGCGGCGTCCGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATA‑TTGAACACGAGATTGCAGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCtt                                                                        >  1:247690/1‑250 (MQ=255)                                                                                                                  gttggttTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGCGCGACACCGGGGGCGCCAGCTACACAGGAAGCGACGGAATTTCTTGTCGAGCATTCCGATTATATCGCGCAGAAAATCGATGATC‑TTCACCACGAGATTGCCGCTTTGCAGGCGAAACGTAc                                                                                                                                              >  1:200179/1‑179 (MQ=255)                                                                                                                      gttTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATAGCGAGCAGAAAATCGATGATA‑TTGACCACGAGAGTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAgg                                                                  <  1:335778/251‑1 (MQ=255)                                                                                                                             cACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTGGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATA‑TTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTg                                                           <  1:140577/251‑1 (MQ=255)                                                                                                                                            gAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTTTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATA‑TGGACCAGGAGATTGCCGATTGGCAGGCGAAACGTTCCCGCCTGGTGCAGCAACATCCGCGAAGTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTg                                                           <  1:330172/236‑1 (MQ=255)                                                                                                                                                      gCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATA‑TTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATctct                                  >  1:143659/1‑251 (MQ=255)                                                                                                                                                                           gCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCgatn                                                                                                                                                                                       <  1:72548/82‑2 (MQ=255)                                                                                                                                                                            cGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATA‑TTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAgn                                                                  <  1:91605/197‑2 (MQ=255)                                                                                                                                                                               cacCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGGAGAAAATCGATGATA‑TTGACCACGAGATTGCCGATTTGCAGGCGAAACg                                                                                                                                                 >  1:306688/1‑115 (MQ=255)                                                                                                                                                                                         ntCAGATATACAGGAAGCGAAGGAAATTCTTGTCGAGCATTACGATAATATCGAGCCGAAAATCGATGATA‑TTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACggagg  >  1:36021/2‑248 (MQ=255)                                                                                                                                                                                                               gAAATNNTTNNNGANNATTACGATAATATCGAGCAGAAANTCGAGGAAA‑ATGAAAAGGAGATTGCAGATNTNCNGGCGNNACGNACCCGGCTGGNGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTggtntn                                                                                  >  2:260381/1‑141 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                           CATTTAATATTTCGGTGCTGGTTGCCGGTCTGACGCCAACGCTGGCGGCCTGGCTGGTCGAAAGCTCGCAGAATCTGATGATGCCTGCCTATTACCTGATGGTAGTGGCGGTGGTTGGTTTAATCACCGGCGTAACCATGAAAGAGACGGCAAATCGTCCGTTGAAAGGTGCGACACCGGCGGCGTCAGATATACAGGAAGCGAAGGAAATTCTCGTCGAGCATTACGATAATATCGAGCAGAAAATCGATGATA‑TTGACCACGAGATTGCCGATTTGCAGGCGAAACGTACCCGCCTGGTGCAGCAACATCCGCGAATTGATGAATAAGCTGAAACGGATGGCCTGATGTGACGCTGTCTTATCAGGCCAATTGAACTCTTAAGGTTCACTTAATCTCTGACGCGCATACTCTCCTCCAGGTTAACGGAGG  >  NC_000913/4331676‑4332107

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑