| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,316,498 | G→A | H241Y (CAC→TAC) | phnM ← | RPnTP hydrolase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,316,498 | 0 | G | A | 93.3% | 37.3 / NA | 15 | H241Y (CAC→TAC) | phnM | RPnTP hydrolase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (7/7); minor base C (1/0); total (8/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGG > NC_000913/4316274‑4316701 | ngCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCgtgggtgg > 1:37373/2‑251 (MQ=255) ngAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATc > 1:214538/2‑251 (MQ=255) cAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTTCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTTCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCTATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCa < 1:19516/250‑1 (MQ=255) aGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAAt < 1:122604/251‑1 (MQ=255) cgccgcGCACAATATTCGGCGCGCCCATCAGCACGTTCATGGCATGCTTTCGCTAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTTGCAAGCTGGTAAGATTCAGCAACGTGTGCGTGGGTGGCGTCATCGTGGCTGGCAAGGGCAATTTTTCGCGCGTGGCGCATGGCGGCTATGGTTTCGCGATTCGGCTGCTACGAGCTTGCGGCGAGCGCCAGTTGCTCTTCTTCGTActgc < 1:138988/251‑1 (MQ=255) gccgcGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGATGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCg < 1:13354/209‑1 (MQ=255) naaTNTTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGt < 2:214538/137‑1 (MQ=255) cgnnnNNATCNGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGACGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAAt < 2:211225/144‑1 (MQ=255) ngcCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAgg > 1:90877/2‑251 (MQ=255) cccATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGCAATTCGGCGATCACGCTGCCACGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTgcggc > 1:133874/1‑200 (MQ=255) gCACGTTCCTGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCATAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTg > 1:353667/1‑251 (MQ=255) ngTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGccc > 1:285492/2‑251 (MQ=255) gCTTCNGCCGCNTNNAACGCGGAGGGAAATTAGGNGATCACGCTGCCAAGNTGGTNAGAttncgn > 2:137752/1‑61 (MQ=255) tCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGGGACCAGCGTGCGGCGAGCGCCAGTTGTTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGATGGAGTATTTGCCCTGATAATATTCGCGATACTTCTcg < 1:137752/249‑1 (MQ=255) gtggtgGGAAATTCGGCGATCACGCTGCCAAGCTGGTCAGCTTCAGCAACGTGGGCGTGGCCGCTGTCATCGTGGCTGGCACGCGCAATTTTTTGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGc > 1:195717/1‑132 (MQ=255) gAAATTCGGCGATCACGCTGCCAAGCTGGTAAGATTCAGCGACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCACTTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGAGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCCTCTGCGCATCAGTGAGGGCGTATTTGCCCTGATAATATTCGCGCTACTTCTCGCGGTTgg > 1:227786/1‑234 (MQ=255) | GGCGACGCGAAATGCCGCATCGAGCAGGCTGGCGGGGTAGTAGTCGGAAGAGAGGATATCCAGCAGGCCAAGCTGCGCCAGTTCACTGGCCGCCACGTTGCCGGAGTGCGAGCCGCCGCGCACAATATTCGGCGCGCCCATCAGCACGTTCATGCCATGCTTGCGCGAGGCTTCCGCCGCTTCGAACGTGGTGGGAAATTCGGCGATCACGCTGCCAAGCTGGTGAGATTCAGCAACGTGGGCGTGGGTGGCGTCATCGTGGCTGGCAAGCGCAATTTTTCGCGCGCGGCACAGGGCGGCGATGGATTCGCGATTCGGCTGCGACCAGCGTGCGGCGAGCGCCAGTTGCTCTTCTTCGTACTGCTGCATCTGCGCATCAGTGAGGGAGTATTTGCCCTGATAATATTCGCGATACTTCTCGCGGTTGG > NC_000913/4316274‑4316701 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |