Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,225,271	G→A	V482I (GTA→ATA)	metH →	cobalamin‑dependent methionine synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,225,271	0	G	A	100.0%	47.5 / NA	17	V482I (GTA→ATA)	metH	cobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/13);  total (4/13)

CAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCG  >  NC_000913/4225027‑4225505                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           cAACATGGAGGAAGGGATGCTCGATGCCGAAGCGGCGATGTTGCGTTTTCTCAATCGGATTGCCGGTGAACCGGATATCGCTCTCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTTAAGTGTATCCAGGGCAAAGGCATTTTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGTGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATgg                                                                                                                                                                                                                                      <  1:261359/251‑1 (MQ=255)       ggATGAAGGGAGGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTAGCCAAGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGCTGCCGTTATCCATCACGCGAAATTCTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCttn                                                                                                                                                                                                                                 <  1:43836/250‑2 (MQ=255)         aTGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATAATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCTTTGAc                                                                                                                                                                                                                              <  1:15109/251‑1 (MQ=255)                                   gcgATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGACGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAAt                                                                                                                                                                                                                                        >  1:251359/1‑215 (MQ=255)                                                                    gAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGCCGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAACGAGGGCGTCGATGCCTTTCTCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATAATCGCGCACGGAAAATCGAGATTTGCCg                                                                                                                                                                               >  1:164751/1‑239 (MQ=255)                                                                             atCGCTCGCGTGCATATTAGGATCGACTCCTCAAAATGGTACTTCATTGAAAAAGGTCTGAAGATTATCCAGGGCAAAGGCAGTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTTCGTCGCTACGGTGCGGCAGTGGTGATAATGGCATGTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATTGAGATGTGCCGGCGTTCGGTCAAAATCCTCAc                                                                                                                                                          <  1:273227/251‑1 (MQ=255)                                                                                     cgTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGGGTAACCAGGGAAAAGGCATTGTTAACGCTATCTCAATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTGGCGGCGCTACGGGGCGGCAGGGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGGCGGGCGTACAAAATCCTCACCGAAGAgg                                                                                                                                                  <  1:109815/251‑1 (MQ=255)                                                                                              tATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTc                                                                                                                                          <  1:56848/250‑1 (MQ=255)                                                                                                      aCTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCaga                                                                                                                                  >  1:286248/1‑250 (MQ=255)                                                                                                                   ggACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACTCGAAATCGGTGCGTCGCTACGGTGTGGCAGTGGTTATATAGGCCTTTTGCGGACAGGGGCAGGGCGATACGCGCGCACGGAAAAGCGAAATTTGGCGTCGGGCGTACAAAATCCGCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCg                                                                                                                    <  1:200720/251‑1 (MQ=255)                                                                                                                        tCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCAcn                                                                                                                                                         <  1:284494/209‑2 (MQ=255)                                                                                                                              aaaaaGGTCTGAAGTGGATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCATTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGTCCCAAACAtn                                                                                                         <  1:90978/251‑2 (MQ=255)                                                                                                                                aaaGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCtt                                                                                                       <  1:17886/251‑1 (MQ=255)                                                                                                                                        gAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTc                                                                                                <  1:124647/250‑1 (MQ=255)                                                                                                                                                                               gATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGACATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATACTcg                                                                                                                                                                                                       >  1:315512/1‑108 (MQ=255)                                                                                                                                                                                                                 gcgAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACgcgc                                                              <  1:95965/211‑1 (MQ=255)                                                                                                                                                                                                                                     cGGTGCGGCAGTGGTGATAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAATAGGTTGGCTTCCCGCCAGAAGATATCATCTGCGACCCAAGCATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACGTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCcg  <  1:250861/251‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                           CAACATGGATGAAGGGATGCTCGATGCCGAAGCGGCGATGGTGCGTTTTCTCAATCTGATTGCCGGTGAACCGGATATCGCTCGCGTGCCGATTATGATCGACTCCTCAAAATGGGACGTCATTGAAAAAGGTCTGAAGTGTATCCAGGGCAAAGGCATTGTTAACTCTATCTCGATGAAAGAGGGCGTCGATGCCTTTATCCATCACGCGAAATTGTTGCGTCGCTACGGTGCGGCAGTGGTGGTAATGGCCTTTGACGAACAGGGACAGGCCGATACTCGCGCACGGAAAATCGAGATTTGCCGTCGGGCGTACAAAATCCTCACCGAAGAGGTTGGCTTCCCGCCAGAAGATATCATCTTCGACCCAAACATCTTCGCGGTCGCAACTGGCATTGAAGAGCACAACAACTACGCGCAGGACTTTATCGGCGCGTGTGAAGACATCAAACGCGAACTGCCGCACGCGCTGATTTCCG  >  NC_000913/4225027‑4225505

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑