Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,224,762 G→A G312D (GGC→GAC)  metH → cobalamin‑dependent methionine synthase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,224,7620GA94.4% 43.9 / ‑6.7 18G312D (GGC→GAC) metHcobalamin‑dependent methionine synthase
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base A (6/11);  total (6/12)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.93e-01

CTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTG  >  NC_000913/4224548‑4225000
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
ctGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCTCCGCGCACCCGAACGCCGGTCTACCCAACGCCTTTGGTGATTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCGGCTGTGACTCCACGCCACAACATATTGCAGCGATGAGTCg                                                                                                                                                                                                               <  1:109801/248‑1 (MQ=255)
   aCCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGt                                                                                                                                                                                                         <  1:327791/251‑1 (MQ=255)
                     gcgcTGGGGCACGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCcgcg                                                                                                                                                                                       >  1:283070/1‑251 (MQ=255)
                                            ccAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGACACCCCGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGt                                                                                                                                                                  >  1:23320/1‑249 (MQ=255)
                                                  cGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGcc                                                                                                                                                          <  1:327633/251‑1 (MQ=255)
                                                         gAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGTCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGTCGCAATCGGATTTTCTCAATATCGTCGTCGGCTGCTGTGACACCACGCCACAACAAATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTc                                                                                                                                                   <  1:231014/251‑1 (MQ=255)
                                                           gCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACAATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGGAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCg                                                                                                                                                 <  1:108696/251‑1 (MQ=255)
                                                                                  ccatcacgctctcgccGAACGCCGGGCTACCCAACGCCTTTGGGGAGTACGATCTCGACGCCGACACGAGGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGc                                                                                                                          <  1:318314/237‑1 (MQ=255)
                                                                                                                 cAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATTGGCTCAAGCGGGTTTTCTCAATATCGTCGGCGGCTTCTGTGACACCACGTCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGc                                                                                                                                                           <  1:153966/187‑1 (MQ=255)
                                                                                                                                                gACACGATGCCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCGCAATATCGTCGACGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGc                                                                                                                                                                                                            <  1:334545/107‑1 (MQ=255)
                                                                                                                                                  cacGATGGCAAAACAGATACGTGAATGGGCGCAGGCGGGTTTTCTCAAGTTCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAAGTCCCGTAGCCTGCCGGTTGGCCGGCCTGGAGCCGCTTAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGTTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAgc                                                          <  1:336146/251‑1 (MQ=255)
                                                                                                                                                        ggCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTg                                                     >  1:160321/1‑250 (MQ=255)
                                                                                                                                                         gCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATAGCGTCGGCGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAgc                                                                                                                                                                                             <  1:196684/113‑1 (MQ=255)
                                                                                                                                                                            gggCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTTACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAATGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGc                                <  1:155293/251‑1 (MQ=255)
                                                                                                                                                                                             ctcAATATCGTCGGCGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCNGAANNNNNGcnn                                                                                                                        >  2:217537/1‑144 (MQ=255)
                                                                                                                                                                                             ctcAATATCGTCGGCGGCTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACNNCCGGa                                                                                                                                                                           >  2:2964/1‑95 (MQ=255)
                                                                                                                                                                                                          gcggcTGCTGTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGtn  <  1:217537/251‑2 (MQ=255)
                                                                                                                                                                                                              ctgctgTGACACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCa         >  1:314600/1‑240 (MQ=255)
                                                                                                                                                                                                                      |                                                                                                                                                                                                                                              
CTGACCTTTGGCCTGAACTGTGCGCTGGGGCCCGATGAACTGCGCCAGTACGTGCAGGAGCTGTCACGGATTGCGGAATGCTACGTCACCGCGCACCCGAACGCCGGGCTACCCAACGCCTTTGGTGAGTACGATCTCGACGCCGACACGATGGCAAAACAGATACGTGAATGGGCGCAAGCGGGTTTTCTCAATATCGTCGGCGGCTGCTGTGGCACCACGCCACAACATATTGCAGCGATGAGTCGTGCAGTAGAAGGATTAGCGCCGCGCAAACTGCCGGAAATTCCCGTAGCCTGCCGTTTGTCCGGCCTGGAGCCGCTGAACATTGGCGAAGATAGCCTGTTTGTGAACGTGGGTGAACGCACCAACGTCACCGGTTCCGCTAAGTTCAAGCGCCTGATCAAAGAAGAGAAATACAGCGAGGCGCTGGATGTCGCGCGTCAACAGGTG  >  NC_000913/4224548‑4225000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑