Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,218,853	C→T	Y86Y (TAC→TAT)	aceK →	isocitrate dehydrogenase kinase/phosphatase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,218,853	0	C	T	100.0%	25.5 / NA	10	Y86Y (TAC→TAT)	aceK	isocitrate dehydrogenase kinase/phosphatase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/4);  total (6/4)

CAAACCATTTTGCAAGGCTTCGATGCTCAGTATGGTCGATTCCTCGAAGTGACCTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTAC  >  NC_000913/4218626‑4219052                                                                                                                                                                                                                                    |                                                                                                                                                                                                        cAAACCATTTTGCAAGGCTTCGATGCTCAGTATGGTCGATTCCTCGAAGTGACCTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTATACCCGGCTGTTGCCGGATTAcn                                                                                                                                                                                   <  1:67815/250‑2 (MQ=255)                     cGATGCTCAGTATGGTCGATTCCTCGAAGTGACCTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTATACCCGGCTGCTGCCGGATTACCCGCGCTTCGAGATTGCgg                                                                                                                                                                 >  1:257222/1‑248 (MQ=255)                                          ccTCGAAGTGACCTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTATACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACt                                                                                                                                         >  1:274506/1‑251 (MQ=255)                                                     ccTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGTCTGGCATGCTGTCCAGCAGGCGATGAAAACCCGTATACATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGGCGCGGCATTTTTACTACGTGTTAAAGAGCATTATACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGagag                                                                                                                                                             >  1:138454/1‑220 (MQ=255)                                                                                                                                                            ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTCCGTGTTAAAGAGCATTATACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCTTACCATTTCCCGCCCGCTGGCGAAAGACTTTCACCCCg                       >  1:52846/1‑251 (MQ=255)                                                                                                                                                            ggAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTATAACCGGCTGTTGCAGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTTTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGCCTTTCACCCCg                       >  1:52854/1‑251 (MQ=255)                                                                                                                                                                                      gCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTATACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGc                                                                                                     <  1:45006/147‑1 (MQ=255)                                                                                                                                                                                                     cgGCATTTTTACTACGTGTTAAAGAGCAGTATACCCGGCTGTTGCCGGATTACCCGCGCTGCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGaccacn                                                                                                                       <  1:33031/114‑2 (MQ=255)                                                                                                                                                                                                      ggCATTTTTACTACGTGTTAAAGAGCATTATACCCGGCTGTTGCCGGCTTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTTACCACCGCCCGCTTACTCCCGAGCGGNCTTNNNNNttnnnnnn                                                                                  >  2:215117/1‑144 (MQ=255)                                                                                                                                                                                                      ggCATTTTTACTACGTGTTAAAGAGCATTATACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTan  <  1:215117/230‑2 (MQ=255)                                                                                                                                                                                                                                    |                                                                                                                                                                                                        CAAACCATTTTGCAAGGCTTCGATGCTCAGTATGGTCGATTCCTCGAAGTGACCTCCGGTGCGCAGCAGCGTTTCGAACAGGCCGACTGGCATGCTGTCCAGCAGGCGATGAAAAACCGTATCCATCTTTACGATCATCACGTTGGTCTGGTCGTGGAGCAACTGCGCTGCATTACTAACGGCCAAAGTACGGACGCGGCATTTTTACTACGTGTTAAAGAGCATTACACCCGGCTGTTGCCGGATTACCCGCGCTTCGAGATTGCGGAGAGCTTTTTTAACTCCGTGTACTGTCGGTTATTTGACCACCGCTCGCTTACTCCCGAGCGGCTTTTTATCTTTAGCTCTCAGCCAGAGCGCCGCTTTCGTACCATTCCCCGCCCGCTGGCGAAAGACTTTCACCCCGATCACGGCTGGGAATCTCTAC  >  NC_000913/4218626‑4219052

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑