Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,193,996	T→G	D70A (GAT→GCT)	thiE ←	thiamine phosphate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,193,996	0	T	G	100.0%	37.1 / NA	12	D70A (GAT→GCT)	thiE	thiamine phosphate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (7/5);  total (7/5)

GTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCC  >  NC_000913/4193763‑4194216                                                                                                                                                                                                                                          |                                                                                                                                                                                                                             gTTCCAGCCTCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCGGCGCGACGTCGATTTCCATATCGTCTTGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGGGACTTGCAAATCTCCCTGCCCCAAATGGACGCCCAACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATcg                                                                                                                                                                                                              <  1:155477/250‑1 (MQ=255)                gTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCCTATCGTCCTGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCCAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGcggcgg                                                                                                                                                                                              >  1:162506/1‑251 (MQ=255)                      aaGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAgc                                                                                                                                                                                        <  1:113293/251‑1 (MQ=255)                                                                                          gcgACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAgt                                                                                                                    >  1:97663/1‑251 (MQ=255)                                                                                              cGTCGATTTCCATATCGTAATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCCGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGATGGAGAgtacgg                                                                                                                >  1:76663/1‑250 (MQ=255)                                                                                                                             ccccAGCCGCCGGCCTCCCGTGCGAGTGGCATGGAGAGCGGCGGCTTCCAAATCTCCCTCCCCCAAATGCGCGCCATTCCCCTGATGCTTGAGCGCCAGCCGCCGTTAAGCTTTGAGAAACAATCGCGCGTTATAGCGGCGGCCCACCGCATTTGCCGCCGCGACCTCGCCTCCCACCGCTTCATCGCGCCGATCTTTGAGGCGTAGGTGGATAGTATGTACGCGTGCATCCAACAGACGTTCGATCCACt                                                                                 <  1:304824/250‑1 (MQ=255)                                                                                                                                         nccTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGTCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTcc                                                                      >  1:285246/2‑250 (MQ=255)                                                                                                                                                   cgGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAg                                                                                               >  1:186445/1‑215 (MQ=255)                                                                                                                                                                            gCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGAGCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCGCGACATCGGCTTCCACCTCTTCAGCGCGCCGATCTTTGAGGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACagg                                   <  1:241996/250‑1 (MQ=255)                                                                                                                                                                                     ccTGCCCAAAAGGGGCGCCATACGCCTGATCCTGGATGGCCGGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACg                                                                                                                                                                         <  1:187566/107‑1 (MQ=255)                                                                                                                                                                                                 ggACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCNNCCACg                                                                                                                                                                         >  2:8484/1‑95 (MQ=255)                                                                                                                                                                                                            gCCTGATGCTTGATCGCCAGCCGCCAGTAAGCGTTGATACACAATCGCGCGTTATCGCGGCGGCCCAGCGCAATTGCCGCAACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGTGTAGCTGGAGAGAACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTtcctcc  >  1:288757/1‑251 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                             GTTCCAGCCCCTGCGGTGCAGAAGGCATCTGTTTGGTTTGCGTCGGGAACACATGTCCCAGCGCGATATAAGAGGGGCGTGCTGCCAGCGCGACGTCGATTTCCATATCGTCATGTGTCGAAACGCCCAGCCGCAGGCCTGCCGCGCGGATGGCATTGAGATCGGTGGCTTGCAAATCTTCCTGCCCCAAATGGACGCCATACGCCTGATGCTTGATCGCCAGCCGCCAGTAATCGTTGATAAACAATCGCGCGTTATAGCGGCGGCCCAGCGCAATTGCCGCCACGACATCGGCTTCCACCTCTTCATCGCGCCGATCTTTGATGCGTAGCTGGAGAGTACGTACGCCTGCATCCAACAGACGTTCGATCCACTGTACGCTGTCCACCACCGGGTACAGTCCTGAACGAAAAGGTACAGGAGGAAAATCAGGCTGATACATCACGCTTCCTCC  >  NC_000913/4193763‑4194216

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑