Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,187,828	G→A	E827K (GAG→AAG)	rpoC →	RNA polymerase subunit beta'

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,187,828	0	G	A	100.0%	39.0 / NA	15	E827K (GAG→AAG)	rpoC	RNA polymerase subunit beta'
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (5/10);  total (5/10)

CGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGACTCCGGTTATCGAGGGTGGTGACGTTAAAGAGCCGCTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGACGTTCTGAAGCCGGGTACTGCTGATATCCTCGTTCCGCGCAACACGCTGCTGCACGAACAGTGGTGTGACCTGCTGGAAGAGAACTCTGTCGACGCGGTTAAAGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGG  >  NC_000913/4187614‑4188059                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        cGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGACTCCGGTTATCAAGGGTGGTGACGTTAAAGAGCCGCTGCGCGATcgcg                                                                                                                                                                                                     >  1:316719/1‑251 (MQ=255)                           gTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGACTCCGGTTATCAAGGGTGGTGACGTTAAAGAGCCGCTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGAc                                                                                                                                                                           <  1:287877/251‑1 (MQ=255)                           gTGAAGGTCTGAACGTACGCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCATCGTCTGGTTGACGGGGCGCAGGACCTGGTGTTTACCGAAGACGATGGTGGTGCCCATTAAGATATCATGATGACACCGGTTATCAAGGGTGGTGACGTTAAAGAGCGGCTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGAc                                                                                                                                                                           <  1:347547/251‑1 (MQ=255)                                           aCTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCCCTGAACACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGACTCCGGTTATCAAGGGTGGTGACGTTAAAGAGCCGCTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGACGTTCTGAAGCCGGGt                                                                                                                                                            >  1:178810/1‑250 (MQ=255)                                                 gTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCAATGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGCATCATGATGACTCCGGTTATCAAGGGTGGCGACGTTAAAGAGCCGCTGCGCGATCGCGTACTGGGTCgtg                                                                                                                                                                                         >  1:169709/1‑215 (MQ=255)                                                                       gCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGACTCCGGTTATCAAGGGTGGTGACGTTAAAGAGCCGCTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGACGTTCTGAAGCCGGGTACTGCTGATATCCTCGTTCCGCGCAacac                                                                                                                               <  1:1979/251‑1 (MQ=255)                                                                                                cACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGACTCCGGTTATCAAGGGTGGTGACGTTAAAGAGCCGCTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGACGTTCt                                                                                                                                                                      >  1:51085/1‑187 (MQ=255)                                                                                                           gCGAACTCCGGTTACCTGACTCGTCTTCTGGTTGACGTGGCGCGGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGACTCCGGTTATCAAGGGTGGTGACGTTAAAGAGCCGCtn                                                                                                                                                                                                               <  1:31668/135‑2 (MQ=255)                                                                                                                                         gTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTGCCCAGGACGGTAGCATGATTACTTCGGTTATCAAGGGTGGGGACGTTAAAGAGCCGCTg                                                                                                                                                                                                               <  1:278618/105‑1 (MQ=255)                                                                                                                                                 gggtcAGGATCTGGTGGTTACCGAAGACGATTTTTGTACCCATGAAGTTATTATGATGACTCCGGTTATCAAGGGTGGTGACGTTAAAGAGGCGCTGCGCGAGCGCGTACTGGGTCGTGTGACTGCTGGATACGTTCTGAAGCCGGGTACTGCTGATATCCt                                                                                                                                              <  1:309171/158‑1 (MQ=255)                                                                                                                                                   cgcAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGACTCCGGTTATCAAGGGTGGTGACGTTAAAGAGCCGCTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGACGTTCTGAAGCCGGGTACTGCTGATATCCTCGTTCCGCGCAACACGCTGCTGCACGAACAGTGGTGTGACCTGCTGGAAGAGAACTCTGTCGACGCGGTTAAAGTACGTTCTGTTGTATCt                                                   <  1:301926/251‑1 (MQ=255)                                                                                                                                                       ggACCTGGTGGTTACCTAAGACGATTGTGATTCCCATGAAGGTATCATGATGACTCCGGTTATCAAGGGTGGTGACGTTAAAGAGCCTCTTCGCGATCGCTTACTGGTTCGTGTAACTGCTGTAGCCCTTCTTAAGCCGGGTTCTGCTGATATCCTCGTTCCGCTCAACACGCTTCTGCATGAACAGTGGGGTGACCTGCTGTAAgaga                                                                                         <  1:74850/209‑1 (MQ=255)                                                                                                                                                       ggACCTGGTGGTTACCGAAGACGATTNTGGTACNNATNAAGGTATCATGATGACTCCNGTNNNCNNNGNAGGTGNCGNNAACGAGCCGNTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGACGTTCTGAAGCCGGGTNCTGCTGATANNCTCGTTCCGCGCAACACGCTGNTGCNNGNNCNgtgnggn                                                                                                         >  2:74850/1‑189 (MQ=255)                                                                                                                                                           cTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGCCTCCGGTTATCAAGGGTCGCGACGTTAAAGAGCCGCTGCGCGATCGCGTACTGGGACGTGTAACTGCTGAAGACGTTCTGAAGCCGGGTACTGCTGATAACCTCGTTCCGCGCAACACGCTGCTGCACGAACAGTGGTGTGACCTGCTGGAAGAGAACTCTGTCGACGCGGTTAAAGTACGTTCTGTTGGTTCTTGTGacac                                           >  1:179658/1‑251 (MQ=255)                                                                                                                                                                                                    cATGATGACTCCGGTTATCAAGTGTGGTGACGTTAAAGAGCCTCTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGACGTTCTGAAGCCGGGTACTGCTGATATCCTCGTTCCGCGCAACACGCTGCTGCACGAACAGTGGTGTGACCTGCTGTAAGAGAACTCTGTCGACGCGGTTAAAGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTgg  <  1:256040/251‑1 (MQ=255)                                                                                                                                                                                                       tatgaCTCCGGTTATCAAGGGTGGTGACTTTAAATATCCGCTGCGCTATCGCTTACTGGGTCGTGTAACTGCTGAAGTCGTTCTGAAGCCGGGTACTGCTGATATCCTCGTTCCgcgc                                                                                                                                    <  1:239438/117‑1 (MQ=255)                                                                                                                                                                                                                       |                                                                                                                                                                                                                                        CGAAACGCCAATCACCGCGAACTTCCGTGAAGGTCTGAACGTACTCCAGTACTTCATCTCCACCCACGGTGCTCGTAAAGGTCTGGCGGATACCGCACTGAAAACTGCGAACTCCGGTTACCTGACTCGTCGTCTGGTTGACGTGGCGCAGGACCTGGTGGTTACCGAAGACGATTGTGGTACCCATGAAGGTATCATGATGACTCCGGTTATCGAGGGTGGTGACGTTAAAGAGCCGCTGCGCGATCGCGTACTGGGTCGTGTAACTGCTGAAGACGTTCTGAAGCCGGGTACTGCTGATATCCTCGTTCCGCGCAACACGCTGCTGCACGAACAGTGGTGTGACCTGCTGGAAGAGAACTCTGTCGACGCGGTTAAAGTACGTTCTGTTGTATCTTGTGACACCGACTTTGGTGTATGTGCGCACTGCTACGGTCGTGACCTGG  >  NC_000913/4187614‑4188059

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑