| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 4,026,650 | G→A | D42N (GAT→AAT) | fre → | NAD(P)H‑flavin reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 4,026,650 | 0 | G | A | 87.5% | 11.3 / ‑3.0 | 8 | D42N (GAT→AAT) | fre | NAD(P)H‑flavin reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/1); new base A (4/3); total (4/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.76e-01 | |||||||||||
GCATATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCC > NC_000913/4026424‑4026880 | gcATATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTtct > 1:16934/1‑251 (MQ=255) aacGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCt > 1:279591/1‑251 (MQ=255) cgTTTGTTTTGCGCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCAGCATCGTGCCAGACGCGGCCTTTTCGTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCg < 1:8376/200‑1 (MQ=255) ggTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAAGCGTGGTCGACATTCCCCACg < 1:113572/251‑1 (MQ=255) ntCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTAAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGCCGCCGGATGAAAAAGGGTTTATCGAGCTGAATATTGGCGCTTCTGCAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCg > 1:70085/2‑251 (MQ=255) ctCGTGCCAGACGCGGCCTTTTCTTTTCGGGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCACTGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGNTTCNNNNNTc > 2:215874/2‑144 (MQ=255) ttttcGTGCTGGTCAGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTACCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGAtt < 1:8792/232‑1 (MQ=255) aGTATTTGATGGTAGTGATGAATGAGCGCGACAAACGGCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGGCGACATTCCCCACGTAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGcc < 1:357233/251‑1 (MQ=255) | GCATATTGACGCCATCTGGGATGAACTGGCTATTTTTAACAACGGTAAAAGCGCCTGATGCGCGTTTGTTTTGCCCTATTTATCGATCCGACAGAGAAAGCGCATGACAACCTTAAGCTGTAAAGTGACCTCGGTAGAAGCTATCACGGATACCGTATATCGTGTCCGCATCGTGCCAGACGCGGCCTTTTCTTTTCGTGCTGGTCAGTATTTGATGGTAGTGATGGATGAGCGCGACAAACGTCCGTTCTCAATGGCTTCGACGCCGGATGAAAAAGGGTTTATCGAGCTGCATATTGGCGCTTCTGAAATCAACCTTTACGCGAAAGCAGTCATGGACCGCATCCTCAAAGATCATCAAATCGTGGTCGACATTCCCCACGGAGAAGCGTGGCTGCGCGATGATGAAGAGCGTCCGATGATTTTGATTGCGGGCGGCACCGGGTTCTCTTATGCC > NC_000913/4026424‑4026880 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |