Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,931,779	T→C	F155S (TTT→TCT)	kup →	K(+):H(+) symporter Kup

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,931,779	0	T	C	100.0%	19.7 / NA	10	F155S (TTT→TCT)	kup	K(+):H(+) symporter Kup
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (8/2);  total (8/2)

CGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCT  >  NC_000913/3931535‑3931938                                                                                                                                                                                                                                                     |                                                                                                                                                                ngggCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGAt                                                                                                                                                           >  1:213549/2‑251 (MQ=255)                                   ngTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTg                                                                                                                         >  1:68251/2‑251 (MQ=255)                                                          aaTACGTCGGCGCGAACCACATCAATGCTGGGGATTACGGGACTAATCGGCGGCAGCTTTTTCTCTGGTGAAGTCGTCACAACACCCGCTATTTCGGAGATGTCAGCAATTGAAGGACTGGCAATAGCCGCCACGCAGCGAGATACCTGCATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCCCGGCTa                                                                                                                                       >  1:207286/1‑214 (MQ=255)                                                                                                              ncAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTAGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTAAAAAAAATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGa                                               >  1:33975/2‑250 (MQ=255)                                                                                                                                                            nntGNNNNNCATNGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGc                                                                                                         <  2:213549/144‑1 (MQ=255)                                                                                                                                                             ntgtaaGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCAACTCTCAATTATCGATCTAACTTTATTATCTATGATTCAAAAACATGGCACGGCTCTGGTCGGTAAGCTGTTTGCGACGATCATGCTGAACTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGTTAACCCGGAAGTGCTGCATGCGCTGAATc                           >  1:68667/6‑223 (MQ=255)                                                                                                                                                                                            ncccGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAgg                                                                                >  1:70132/2‑139 (MQ=255)                                                                                                                                                                                             cccGCAGCTGGATACCTGGATAGTTACACTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCCCGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCt  >  1:292832/1‑216 (MQ=255)                                                                                                                                                                                                               gATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATCTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTg                                                                                                                     >  1:138049/1‑83 (MQ=255)                                                                                                                                                                                                                                tnATNGTTCTNACTTNATTATCTATGATTCANAAACATGGCACGGCTATGGNNGNTAAGCNGTTTg                                                                                                                     <  2:138049/66‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                CGGGCAGATAACGCCGGTGAGGGGGGGATCCTGACGTTGATGTCGCTTGCCGGGCGTAATACGTCGGCGCGAACCACATCAATGCTGGTGATTATGGGGCTAATCGGCGGCAGCTTTTTCTATGGTGAAGTCGTCATAACACCCGCTATTTCGGTGATGTCAGCCATTGAAGGTCTGGAAATCGTCGCCCCGCAGCTGGATACCTGGATAGTTCCCCTCTCAATTATCGTTCTCACTTTATTATTTATGATTCAAAAACATGGCACGGCTATGGTCGGTAAGCTGTTTGCGCCGATCATGCTGACCTGGTTTTTGATTCTGGCAGGACTGGGGTTACGTAGCATTATTGCTAACCCGGAAGTGCTGCATGCGCTGAATCCAATGTGGGCGGTGCATTTCTTCCT  >  NC_000913/3931535‑3931938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑