| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 410,666 | C→T | P175S (CCT→TCT) | mak → | fructokinase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 410,666 | 0 | C | T | 85.7% | 24.2 / ‑2.7 | 14 | P175S (CCT→TCT) | mak | fructokinase |
| Reads supporting (aligned to +/- strand): ref base C (1/1); new base T (3/9); total (4/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.05e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGG > NC_000913/410434‑410879 | gNGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTaaa > 1:278067/1‑250 (MQ=255) aaGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGTTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGGGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATTGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTAGTTCGGTAAACAAGGTTGTATTGAAACCTTTATGTCAGTCACGGGATTCGCGATGGAt < 1:293088/251‑1 (MQ=255) cAGACGTTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGTCATTCAATGGTCGGGCGCATATCGGCGGCAATGGCACGTCAGGTGAGTGGGGCCACAATCCGCTACCGTGGATGGACGAAGACGAACTGCTTTATCGCGAGCAAGTCTCTTGTTATTTCTGGAAACAAGGTTGGATTGAAACCTTTTTTTCGGGCACGGGATTCGCGATTGATTATCGTCGTTTGAGc < 1:320599/237‑1 (MQ=255) gaaggTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAg < 1:353082/248‑1 (MQ=255) ggATGCGGCGCGGGCGTGGCATTCAATGTGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTAGTGCGGTAAACAAGGTTGTATTGAAGCCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGttt < 1:338222/203‑1 (MQ=255) ggggCGTGGCATTCAGTGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGGTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGGTATGGCGGTAAACAAGGGTGTATTGAAACCTTTATTTCGGGGACGTGAGGCGCGATGGATTATCATCTTGTGAGCGGACATGCGCTGAAAGGCAGTGAAAGTATCCGCCTGGTTGAAGAAAGCGATCCg < 1:237390/250‑1 (MQ=255) gggcgggcGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGTGCACGGTATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTTGTTTAAGAAAGCGAGCCGGTAGCTGAACTGGCAtt < 1:127904/251‑1 (MQ=255) cgggcgCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAGCCTTGATGTCGGTTACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAg < 1:313900/193‑1 (MQ=255) cgcATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACCCAATCCGCCACCGTGCAAGGCAGCACAACAACCGCGTTATCGCGAGGAAGTACATTACTATTGCGGTAAACAAGGTTCTATTGTCACCTTTATTTTGGGCACGGGATTCCCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGGAGCGGAACTGGCATTGCggcgg > 1:188334/1‑247 (MQ=255) cGGCAGGGGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGGTATTGCGGTAAACAAGGGTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTg < 1:163518/149‑1 (MQ=255) gnACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGc > 1:277077/1‑206 (MQ=255) natggaCGAAGACGAACTGCGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCgg > 1:64404/2‑251 (MQ=255) cGTTATCGCGAGGAAGTCTCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTAGTTCGGGCACGGGATTCGCTATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGc < 1:229061/158‑1 (MQ=255) gTTATCGCGAGGAAGGCTCTTGTTAGTGCGGTAAACAAGGTTTTATTGAAACCGTTATGTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCt < 1:306077/109‑1 (MQ=255) | GCGGGAAGTGCGGCTGGCAAATGACGCTAACTGTCTGGCGGTTTCAGAAGCAGTAGATGGCGCGGCAGCGGGAGCGCAGACGGTATTTGCCGTGATTATCGGCACGGGATGCGGCGCGGGCGTGGCATTCAATGGGCGGGCGCATATCGGCGGCAATGGCACGGCAGGTGAGTGGGGACACAATCCGCTACCGTGGATGGACGAAGACGAACTGCGTTATCGCGAGGAAGTCCCTTGTTATTGCGGTAAACAAGGTTGTATTGAAACCTTTATTTCGGGCACGGGATTCGCGATGGATTATCGTCGTTTGAGCGGACATGCGCTGAAAGGCAGTGAAATTATCCGCCTGGTTGAAGAAAGCGATCCGGTAGCGGAACTGGCATTGCGTCGCTACGAGCTGCGGCTGGCAAAATCGCTGGCACATGTCGTGAATATTCTCGATCCGG > NC_000913/410434‑410879 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |