Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,926,991	G→A	L5L (CTG→TTG)	asnC ←	DNA‑binding transcriptional dual regulator AsnC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,926,991	0	G	A	100.0%	47.6 / NA	18	L5L (CTG→TTG)	asnC	DNA‑binding transcriptional dual regulator AsnC
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/9);  total (9/9)

GCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCG  >  NC_000913/3926750‑3927197                                                                                                                                                                                                                                                  |                                                                                                                                                                                                               gcgGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTc                                                                                                                                                                                                       >  1:135064/1‑251 (MQ=255)                  ggCGCTCTTTAATATAATGCCGATAAAGCAGCCTACTTCATAACCGAGCTGTGTCGGGCTGACATCAATACCCTCCCCGGTAATGATCCCCGTCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGGCTGACGCCAAATTGTGTCTCCAGTTCGTCGTAAGTTGTGCGCGCATTGCCCATTAATGCTTCCATGATGCCACTGTTCAGATTGTCTATCAAATAATTTTCCATAGGATTTTCTTATg                                                                                                                                                                                      <  1:267985/251‑1 (MQ=255)                                                                               aCATCAATACGCGCCCCGTTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTGTTAATGAATCAAAAGTGATTTAGGcn                                                                                                                         <  1:92545/251‑2 (MQ=255)                                                                                naTCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCtt                                                                                                                        >  1:217528/2‑251 (MQ=255)                                                                                                         ccccGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCAtgtg                                                                                                <  1:19052/250‑1 (MQ=255)                                                                                                           ccGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCACATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATgtgtgt                                                                                             >  1:278694/1‑251 (MQ=255)                                                                                                                  cgtCATTTTCTCGACTCGAACGTGAATCTTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAAGGCTTCCAGGATGCCACGGGCCAGATTGTCGATCAAATAATTTTCCATAGGATGTTCTTATGCGGATTGATGACTCATTCTATTTTAGCCTTCGTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGaa                                                                                                               <  1:199100/224‑1 (MQ=255)                                                                                                                               aCTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATc                                                                          <  1:124910/250‑1 (MQ=255)                                                                                                                                      cGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCNTAAGCGGTGCNCGCNNTGCCNANTNATGCTTCCAGGATGCCACGGTCCAGATTGNCGATCAAATAATTTTCCATAGGANNTTNNNATNNGGAtt                                                                                                                                                                                <  2:267836/141‑1 (MQ=255)                                                                                                                                      cGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGAtt                                                                                                                                                                                >  1:267836/1‑141 (MQ=255)                                                                                                                                       gTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGAGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGCTGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGACTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAAc                                                                 >  1:200227/1‑251 (MQ=255)                                                                                                                                                              cAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGTCCATTAATGCTTCAAGCATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCCTTCTATTTTAGCCTTCTTTTTTAATGAATCACAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCCTCAGCAACAGGCCGCAGGCGTATAAAAAATg                                          >  1:264590/1‑251 (MQ=255)                                                                                                                                                                       tCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATGCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCTTGTGGGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACgn                                                          <  1:69220/226‑2 (MQ=255)                                                                                                                                                                         gCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGCTTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCtt                                >  1:131550/1‑250 (MQ=255)                                                                                                                                                                                   cGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGATTACATTGCCaa                      >  1:10802/1‑250 (MQ=255)                                                                                                                                                                                     tAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCCTATGCTGATTGATGATTCATTCTATTTTAGCCGTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTTTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGATTATAAAAAATg                                          <  1:105936/228‑1 (MQ=255)                                                                                                                                                                                         nnnnnnnnnnaTNNNNNATTNATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGa                                                                                                                <  2:217528/144‑1 (MQ=255)                                                                                                                                                                                                                            ccACGGTCCAGATTGTCGATCAAATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTTTAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCg  >  1:280728/1‑229 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                               GCGGAAGGGTAGTCTTTGGCGCTCTTTAATATAATGCCGATAAAGCAGCCTACGTCATAACCGAGCTGCTTCGGGCTGACATCAATACGCGCCCCGGTAATGATCCCCGCCTGCTTCATTTTCTCTACTCGAACGTGAATCGTCCCCGGACTGACGCCAAATTGTTTCGCCAGTTCGGCGTAAGCGGTGCGCGCATTGCCCATTAATGCTTCCAGGATGCCACGGTCCAGATTGTCGATCAGATAATTTTCCATAGGATTTTCTTATGCGGATTGATGATTCATTCTATTTTAGCCTTCTTTTTTAATGAATCAAAAGTGAGTTAGGCTTTTTATTGAATGATTATTGCATGTGTGTCGGTTTTTGTTGCTTAATCATAAGCAACAGGACGCAGGAGTATAAAAAATGAAAACCGCTTACATTGCCAAACAACGTCAAATTAGCTTCG  >  NC_000913/3926750‑3927197

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑