Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,817,167	C→T	T164T (ACC→ACT)	yicC →	putative RNase adaptor protein YicC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,817,167	0	C	T	100.0%	29.6 / NA	11	T164T (ACC→ACT)	yicC	putative RNase adaptor protein YicC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/5);  total (6/5)

GCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAGGGGAAATCAACCCGGTTGATATTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTG  >  NC_000913/3816928‑3817401                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           gCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAGGGGAAATCAACCCGGTTGATATTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACTGCCGAAGTGGt                                                                                                                                                                                                                                 >  1:247817/1‑251 (MQ=255)                               gTAAAAATGCAGAGTGACGAAGGGGAAATCAACCCGGTTGATATTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACTGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCt                                                                                                                                                                                                   >  1:107720/1‑251 (MQ=255)                                                             aaCCCGGTTGATATTCTACGCTGGCCGGGCGTGATGGCCGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAACTTCTAGAGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGAACTGAAAGAATTGATCGAGCAGCGTCTGGAAGGCGTCACTGCCGAAGTGGTCAACGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGa                                                                                                                                                                      >  1:56382/1‑250 (MQ=255)                                                                         atTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGGAGCGTCTGGAAGGCGTCACTGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGc                                                                                                                                                         <  1:112242/251‑1 (MQ=255)                                                                                                                            acgtgtgctcttctgatcGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACTGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGGTCAGGTGCAACTAGAAAACAACCGTCTGGAGCAGGATCTGGTTCTGCTGGc                                                                                                      <  1:242008/235‑1 (MQ=255)                                                                                                                                                         nggTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACTGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTgg                                                                         >  1:213156/2‑251 (MQ=255)                                                                                                                                                                                    gcgcgAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACTGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAag                                                 >  1:15736/1‑248 (MQ=255)                                                                                                                                                                                             cGAAGGTCAGTCACTGAAAGCATTGATCTAGCAGCGGCTGTAAGGCGTCACTGCCGAAGTTTTCAAAGTCCGCTCCCATAGTCCTGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATg                                                                                                                                                          <  1:348145/134‑1 (MQ=255)                                                                                                                                                                                                          cTGAACGCATTGATCGAGCAGCGTCTGGCAGGCGTCACTGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGa                                                                                                                                                               >  1:269422/1‑116 (MQ=255)                                                                                                                                                                                                                         gAGCAGCGTCTGGAAGGCTTCACTGCCGAAGTGGTCAAAGCCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCGGGTCGCGAAGCTGGAAGATGCGCAGGTGCAACTGGAAAACAACCGTCTGGATCAGGAACTGGTTCTGTTGGCACAACGAATTGACGTTGCCAAAGAACTTGATCGCGCCGAAGCGCATGTCAAATAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGgtcg         <  1:183128/251‑1 (MQ=255)                                                                                                                                                                                                                                gTCTGGAAGGCGTCACTGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTg  <  1:511/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                           GCTAAACAGCTGGTAACTGCCGCGAACTGGGTAAAAATGCAGAGTGACGAAGGGGAAATCAACCCGGTTGATATTCTACGCTGGCCGGGCGTGATGGCAGCCCAGGAGCAGGATCTTGACGCCATTGCCGCTGAAATTCTCGCGGCGCTGGATGGTACGCTGGACGACTTTATTGTCGCGCGCGAAACCGAAGGTCAGGCACTGAAAGCATTGATCGAGCAGCGTCTGGAAGGCGTCACCGCCGAAGTGGTCAAAGTCCGCTCCCATATGCCGGAAATCCTGCAATGGCAGCGTGAGCGTCTGGTCGCGAAGCTGGAAGATGCTCAGGTGCAACTGGAAAACAACCGTCTGGAGCAGGAACTGGTTCTGCTGGCACAACGAATTGACGTTGCCGAAGAACTGGATCGCCTCGAAGCGCATGTCAAAGAGACCTACAACATTCTGAAGAAAAAAGAAGCGGTTGGTCGTCGTCTG  >  NC_000913/3816928‑3817401

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑