Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,773,1760CT100.0% 70.7 / NA 27S299F (TCT→TTT) mtlAmannitol‑specific PTS enzyme IICBA component
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (11/16);  total (11/16)

AGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTG  >  NC_000913/3772949‑3773390
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                      
aggtATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCGCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGac                                                                                                                                                                                                <  1:114932/251‑1 (MQ=255)
          gggctgctGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGAGCGGATATCCTCCACTTCCTGGGGGGTTTCAAAAAAATCAACTTCCCGTATCGGCTGATGAATCCGCGTCTGATCCTCACAGTAATCCTCGGAGGTGTGACTGGCGGGTTCACGCTTGCACTCCTGGGTGGGTGG‑CTGGCTTCTTCGGAATATCCGG‑TTTTATCCTTGCTGTAATGGCGATGaccaccacagg                                                                                                                                                                                       >  1:177282/3‑241 (MQ=255)
            gctgctGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGc                                                                                                                                                                                    <  1:268787/251‑1 (MQ=255)
                     ntACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGacac                                                                                                                                                                                              >  1:214188/2‑232 (MQ=255)
                     gTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGCTGacac                                                                                                                                                                                              >  1:161763/1‑232 (MQ=255)
                                             cgcTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTAACGTATGTGCTGATGAATCCGCGTCTGATCCTCGCCGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGGGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTgcggc                                                                                                                                                   >  1:258137/1‑251 (MQ=255)
                                                   aCAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCGGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TGTTATCCTTGCTGTACTGGCGAGGACTCCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGc                                                                                                                                             <  1:164868/251‑1 (MQ=255)
                                                        ccgcgggcggTGCGGCAATCATCCACTCCCTGGGGTGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCGCGCTGACTATCCTGGG‑CGGTGGGCTGGTTTCTCCTGCATCTCCGG‑TTTTATCCTTGCTGGACTGGCGATGACACCAAAAGGTGc                                                                                                                                                                                    <  1:230179/205‑1 (MQ=255)
                                                                      gCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACAGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTctct                                                                                                                          >  1:76072/1‑251 (MQ=255)
                                                                               cACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCGTCTCCGG‑TTTTATCCGTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCGAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTg                                                                                                                 <  1:350823/251‑1 (MQ=255)
                                                                                aCTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGCGCTGATGAATCCCCGTCTGATCCTCTCAGTCATCCTCGGCGGTCTGACTGGCGTGCTCACGCTGAATATCCTGGG‑AGGTGGCCTGGTTTCTCCGGCCTCTCCGG‑TTTTTTCCTTGCTGTACTGGCGCTGACCCCCAAAGGt                                                                                                                                                                                      >  1:360513/1‑181 (MQ=255)
                                                                                   tCCTGGGGGGTATCCACGAAATCTACTGCCCGTATGTGCTGATGAATCCGCGTCTGAGCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACGGGCGATGACACCAAAAGGTGCTTTCTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCt                                                                                                               <  1:315833/249‑1 (MQ=255)
                                                                                                      nnnnnnntnccnngaTGNGCTGATGAATCCGCGTCTGATCCTNGCAGGCATCCTCGGCGGTATGACTGGCGTGTNCNCGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTNATCCTTGCTGNACTNNCGATNANANCAAAAGGTGCTTACTTCGCTAACATCGCGNGTGTGTGTGCGGCGATGGCTGTCNNCTNNNTTNNCTCTg                                                                                                                         <  2:256007/206‑1 (MQ=255)
                                                                                                              tNNNNNATGNGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGacac                                                                                                                                                                                              <  2:214188/143‑1 (MQ=255)
                                                                                                                               aaTCCGCGTCTGATCCTCGCAGTCATCCGCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGc                                                                                                                                             <  1:254907/175‑1 (MQ=255)
                                                                                                                                      ntCTGATCCTCGCAGTCATCCTCGGCGGAATGACTGGCGTGTTCACGCTGACACTCCTGGG‑CGGGGGTCTGGTTTCTCCGGCCTCACCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAAGTGCTTCCTTCGCTAACATCGCGGGTGTGGGTGCGGCGGTGGc                                                                                                                                             >  1:43519/2‑168 (MQ=255)
                                                                                                                                                            ctgcggTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTGCTGGCGAGGACACCAAAAGAGGCTTGCTTCGCTAACATCGCGGGTGTGTGTTCGGCGATGGGTGTCTCCTTCGTTGGCTCTGCTATTTGGCTTAAATCCAGCATAGTGAAATAAGAAGATGATTTTGAAGCAGCAACTCGTCGTATGCAGGTCATGAAAGCTGAg                                      <  1:234861/247‑1 (MQ=255)
                                                                                                                                                                 gTATGNNTGNNNTGNNCACGCTGACTATCCTGGG‑CGGTGNTCTGGTTTCTCCGGGCTC‑CCCGGTTTTATNCNTNCTGTNNTGGNGATGACACCANAAGGGGCTTACTTCGCCAACATCGCGGGTGGGGGCGCGGCAACGGCTGNGNTCTTCGCTGTCGCTGCTATTTAGCTGAAAAANAGTAAAGCGAAAGAAAGAGATGAGNTTGan                                                                          >  2:254705/1‑207 (MQ=255)
                                                                                                                                                                 gTATGNNTGNNNTGNNCACGCTGACTATCCTGGG‑CGGTGNTCTGGTTTCTCCGGCATCTCCGG‑TTTTATNCNTNCTGTNNTGGNGATGACACCANAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGNCNCCTTCGTTGTCTCTGCTATTTTGCTGAAAACNAGCAAAGTGAAAGCAGAAGATGATNTTGAnncancn                                                                    >  2:254706/1‑213 (MQ=255)
                                                                                                                                                                         ggCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGcn                                                                                                                                                                                                     <  1:221114/77‑2 (MQ=255)
                                                                                                                                                                         ggCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCg                                                                                                                                                                                                     >  2:221114/1‑77 (MQ=255)
                                                                                                                                                                                               tNGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGG‑TTTTATCNTTGCNGNNCTNGNGANGacac                                                                                                                                                                                              <  2:161763/62‑1 (MQ=255)
                                                                                                                                                                                                ggg‑CGGTGGTCTGTTTTCTCCGTCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAATAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGAtgtg  <  1:254705/249‑1 (MQ=255)
                                                                                                                                                                                                ggg‑CGGTGGTCTGGTTTCTCCGGCATCGCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGAGGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGAtgtg  <  1:254706/249‑1 (MQ=255)
                                                                                                                                                                                                           tnggNNTCTCNGGCATCTCCGG‑TTTTATCCTTGCTNTACTGGCGATGACACCAAAAGGTGCTTACTTNGNTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCNGCTATTTTGCNGAANNCCAGNANANTGAAATAAGAAGATTATATTGAAGCAGCANCTCGTCGTATGCAGGACATGAAANNTGNNNCTNNAGgcg                            <  2:258137/211‑1 (MQ=255)
                                                                                                                                                                                                                 tctcCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGNAAANNNNnagnnnnnn                                                                                     >  2:213912/1‑144 (MQ=255)
                                                                                                                                                                                                                 tctcCGGCATCTCCGG‑TTTTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAgn                              <  1:213912/205‑2 (MQ=255)
                                                                                                                                                                                                                                    |                                                                                                                                                                                                                      
AGGTATGGGCGTGCTGCTGGCGTACATGTTCTTTGGTCGTGGTAGCGCTAAACAGTCTGCGGGCGGTGCGGCAATCATCCACTTCCTGGGGGGTATCCACGAAATCTACTTCCCGTATGTGCTGATGAATCCGCGTCTGATCCTCGCAGTCATCCTCGGCGGTATGACTGGCGTGTTCACGCTGACTATCCTGGG‑CGGTGGTCTGGTTTCTCCGGCATCTCCGGGTTCTATCCTTGCTGTACTGGCGATGACACCAAAAGGTGCTTACTTCGCTAACATCGCGGGTGTGTGTGCGGCGATGGCTGTCTCCTTCGTTGTCTCTGCTATTTTGCTGAAAACCAGCAAAGTGAAAGAAGAAGATGATATTGAAGCAGCAACTCGTCGTATGCAGGACATGAAAGCTGAGTCTAAAGGCGCATCTCCGCTGTCTGCTGGCGATGTG  >  NC_000913/3772949‑3773390

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑