Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,730,560	C→T	W69* (TGG→TAG)	xylA ←	xylose isomerase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,730,560	0	C	T	100.0%	34.7 / NA	14	W69* (TGG→TAG)	xylA	xylose isomerase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/5);  total (9/5)

TCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGG  >  NC_000913/3730360‑3730802                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   tCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACGTCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCACCCCGTTCcagca                                                                                                                                                                                                   <  1:177547/250‑1 (MQ=255)                               ggACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACgn                                                                                                                                                                    <  1:63763/251‑2 (MQ=255)                                                     attattGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATGGAAGAAGCACTAAAATGGCACATGTAACTAGTGGAAAAACTCAAATATGACATCTGCTTTACGCTTCGCCAACGCCAGTGCATCAAAAGGCTGCTGCTACGGACGATTAAAAGCCCCCACACCAAACATATCCGCACCGTTCCAGCCGAAGGTGTGCCAGTACCAGGCGGCAAACCGCAAGTGCTCTTcaaaacg                                                                                                                                                   >  1:326247/1‑240 (MQ=255)                                                                                                   acaTCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGtt                                                                                                >  1:19753/1‑251 (MQ=255)                                                                                                       cGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTCCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTtcgtcg                                                                                                                             >  1:51551/1‑218 (MQ=255)                                                                                                        gTGGAAGCAATAACGTGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAgg                                                                                           <  1:24580/251‑1 (MQ=255)                                                                                                          ggAAGCAATAAAGTGGCACATGTAACTTGTGGAAAAACTCAAATGCGCCATCTGCTTTACGCTTCGCAAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTCGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGACTGCTAACGGGTTTGAGGAt                                                                                         >  1:269342/1‑251 (MQ=255)                                                                                                          ggAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACACAAATGAGACATATGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGATGCTACGGACGATTAAACGCCCCCACACCAAACATATACGCCCCGTTCCAGCAGAAGGTGAGCCAGTAGCAGGCGGCAAAAAGCAAGTGCTCTTCCATACGATAACCCAACACCAGTTCGTCGGGAATGTAGTGACGGAATGCTAACGGGTTTGAGGAt                                                                                         >  1:269338/1‑251 (MQ=255)                                                                                                                                          aaaaaCTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACggg                                                                                                  >  1:27293/1‑210 (MQ=255)                                                                                                                                             aaCTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTTACGTAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGtn                                                      <  1:34530/251‑2 (MQ=255)                                                                                                                                                              tGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGAACGAGCTGGTCAAAATAGGCTTGCatat                                     >  1:27616/1‑251 (MQ=255)                                                                                                                                                                                      tGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTncgtnnnnngtnn                                                                                                                      >  2:220251/1‑137 (MQ=255)                                                                                                                                                                                      tGCCTCACCAGGCTGCTGCTACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAgn                                              <  1:220251/218‑2 (MQ=255)                                                                                                                                                                                                 nctgctgCTACGGACGATTAAACGCCCCCACACCAAACATCTCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTgatgg  >  1:91373/2‑251 (MQ=255)                                                                                                                                                                                                         |                                                                                                                                                                                                                                                   TCACGCCGCTCTCTTCTTGCTTGCCTGCCAGGACATCAACCATTTGCGCAAAATTATTGATGTACTCTTTTAACGACGCGCCCTCAGGGGAAACATCCACATCGTGGAAGCAATAAAATGGCACATGTAACTTGTGGAAAAACTCAAATGCGACATCTGCTTTACGCTTCGCCAACGCCAGTGCCTCACCAGGCTGCTGCCACGGACGATTAAACGCCCCCACACCAAACATATCCGCCCCGTTCCAGCAGAAGGTGTGCCAGTAGCAGGCGGCAAAACGCAAGTGCTCTTCCATACGCTTACCCAACACCAGTTCGTCGGGATTGTAGTGACGGAATGCTAACGGGTTTGAGGATTTTGAGCCTTCATAACGAACGCGATCGAGCTGGTCAAAATAGGCTTGCATATTGAACTCCATAATCAGGTAATGCCGCGGGTGATGG  >  NC_000913/3730360‑3730802

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑