Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	400,444	G→A	T160T (ACC→ACT)	ddlA ←	D‑alanine‑‑D‑alanine ligase A

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	400,444	0	G	A	100.0%	30.7 / NA	14	T160T (ACC→ACT)	ddlA	D‑alanine‑‑D‑alanine ligase A
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (11/3);  total (11/3)

GCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACC  >  NC_000913/400202‑400690                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       ncTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGCACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGtaataa                                                                                                                                                                                                                                                 >  1:65367/2‑250 (MQ=255)              cGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAGCGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCACTTGCGTAATGTTCTTCACTGGTTACTTTGCTGACACCAAAAGAAGAGCCCTGATTCGCCGGTTTTACAAACAGTGGTACCCCCAGTTTAGCCTCCACTTCGGCAAACCTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATCAATGGCGCAATGTTc                                                                                                                                                                                                                                   >  1:56164/1‑251 (MQ=255)                                   cGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAAATCGCAAGCCAGATAGACGGCAATAGAGTACTGTACTTAAATGGTTACTTTGCTGACAAAATCAGAAGAGACCTGATTAGCCGGTTTTACAACCACTGGTGACCCCAGTTTAGACTTCACTTCGGCAAAACTAATGTTGTGACGATTTGCGCGCGTCAGAGTAATACATGGCGCAATGTTCAGCACGGCATCGCGTAGc                                                                                                                                                                                                                 >  1:228628/1‑248 (MQ=255)                                                                         ttATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAg                                                                                                                                                                         <  1:13687/250‑1 (MQ=255)                                                                                              aGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGCGCCCTGATTAGCCGGATTTACAAACAGCGCTAACCCCAGATTCGACTACACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACaa                                                                                                                                                    >  1:172497/1‑250 (MQ=255)                                                                                                                  ntGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACCGTGGTAACACCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATAAATGGCGCAATGCTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGc                                                                                                                                                                        >  1:41205/2‑210 (MQ=255)                                                                                                                     ttcttcACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATAAATGGCGCAATGTTCAGCCCGTCATCGCGTAGCAGACGTGTGGTGACATCTTTGTCCATACAGGCTGTTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCgcagc                                                                                                                             <  1:191272/250‑1 (MQ=255)                                                                                                                                              ccAACAGCAGACCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGCCTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACCAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGACCCATCTTCGcc                                                                                                   >  1:117524/1‑251 (MQ=255)                                                                                                                                                         ncccTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATAACTGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATc                                                                                                                                                                                                >  1:67063/2‑147 (MQ=255)                                                                                                                                                         gCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGt                                                                                        >  1:293771/1‑251 (MQ=255)                                                                                                                                                                                                     aCTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCg                                            >  1:57928/1‑251 (MQ=255)                                                                                                                                                                                                        ccACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGAGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGGGACCCGCAGCAGTCCCTGCAAGGAACCATCTTCGCCCATCGTATCGTGGACAATCTGGAAAATGACATCCACCGTCGGCAACGGCTGACCGtt                                          <  1:144209/250‑1 (MQ=255)                                                                                                                                                                                                                                        gcgcgcGTCAGAGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGCCGTTTGGTGACCTCTTTg                                                                                                                                                                                            >  1:177888/1‑72 (MQ=255)                                                                                                                                                                                                                                               tCAGAGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGAAGCAGACGTTTGGTGAAATCTTTGTCCCTCCAGGCTGCTGAAGCAAGAACATCAGAACCTACAAACGGAAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTAGGCAACGGCTGAACGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCAcc  >  1:179798/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                       GCTTGCGGATTGTCGTTGCCCAGAACTGCGCATTCGATCTCACGACCTTTGATCCCTTGCTCAACGATCACTTTATGATCGAACTCGAACGCCAGATCGACGGCAATTGCGTACTGTTCTTCACTGGTTACTTTGCTGACACCAACAGAAGAGCCCTGATTAGCCGGTTTTACAAACAGTGGTAACCCCAGTTTAGACTCCACTTCGGCAAAACTAATGTTGTGACGATTGGCGCGCGTCAGGGTAATAAATGGCGCAATGTTCAGCCCGGCATCGCGTAGCAGACGTTTGGTGACATCTTTGTCCATACAGGCTGCTGAAGCCAGAACATCAGAACCTACAAACGGTAAATTGGCGACCCGCAGCATTCCCTGCAAGGAACCATCTTCGCCCAGCGTACCGTGGACAATCGGGAAAATGACATCCACCGTCGGCAACGGCTGACCGTTTTGCGCGTCGATAAGCTGATGCTCATGTTTACCTGGCACC  >  NC_000913/400202‑400690

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑