Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,719,429	G→A	intergenic (‑385/‑49)	yiaF ← / → yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,719,429	0	G	A	100.0%	54.1 / NA	19	intergenic (‑385/‑49)	yiaF/yiaG	DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/10);  total (9/10)

GCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGC  >  NC_000913/3719223‑3719659                                                                                                                                                                                                               |                                                                                                                                                                                                                                       gCCTTTTTTGTAGCCAGAGATTTCCGCCTTCGAAGAAGGTTAGATTTTCGAAATCTTTACAAAACCGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGtt                                                                                                                                                                                            >  1:109613/1‑251 (MQ=255)               cAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGGGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAatat                                                                                                                                                                               >  1:307566/1‑250 (MQ=255)                                 cAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAgn                                                                                                                                                            <  1:216495/251‑2 (MQ=255)                                 cAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGc                                                                                                                                                            >  1:279864/1‑251 (MQ=255)                                 cAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGAGGTAAGAGATATATCGGCCTTTATCAGATCGTTAGGTGGCTATAAGTCAAGCTAACTGACAGGCTGACTCGACTCATAGCAAGAGCATGCCCTGACTTAACCCCGCTGTGTCTGCTTTTCCCGACTCTTCTTAATAAGCTTCGATGCAATTAACGATCACGCAGTGTGATATGAGGAGTTTTCAATGGAATATACAGATCCAATGCATGAGc                                                                                                                                                            >  1:269078/1‑251 (MQ=255)                                                                            agagATATATCGGCATTTATCAGATCTTTAGTTGGCTATAAGTCACGCTAAATGACAGGGTGAATCGAATCATAGCCATTGCATGCTCTTACTGCACCCCGCAGTGTCTGGTTTGCCCGACTGTCCTTAATAAGCTTCGATTGAATTCACGATCCCGCAGTGGGATTTGAGTAGGTTTTAATGGAAGATAAAGATCCAACGCATGAGCTGGTGGGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCa                                                                                                                 <  1:353038/251‑1 (MQ=255)                                                                                                          ngTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTc                                                                                   >  1:220674/2‑251 (MQ=255)                                                                                                                                 gACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCAGGAGCTGTTGAGCAGCTTGGAACAGATTTTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTAc                                                            <  1:190624/251‑1 (MQ=255)                                                                                                                                                   aTAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGcac                                                                                              >  1:137823/1‑199 (MQ=255)                                                                                                                                                     cgnnggatnctGCCCTGACTACACCCCGCTGTCNCTCCTTTTCCTGTCTATTCTTAATAAGCTTCNCNGCAATTCACGATCCCGCAGTGTGATTTGTGGAGTTTCCAATGGAATAANAAGACCCAATNCATNNGCTGNTNANCAGCCTGGAACAGATTGTTTTTAAAGAGGNAACGCAGAAAATTACCCTGACGCNNAGNNNAANNTCCTg                                                                                <  2:269078/201‑1 (MQ=255)                                                                                                                                                      gCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTAAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCATGGAACAGATTGTTTTTAAAGATGAAACGCAGATAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTaaaa                                        >  1:197984/1‑250 (MQ=255)                                                                                                                                                                  ccTGACTTCACCCCGCTGAGTCTGCTTTTCCCGACTATTCTTAATAAGATTCGCTGGAGTTCACCATCCCGCAGTGTGGTTTGAGGCGTTATCAATGGAATATAACGATCAAATGCATGAGCTGTTGAGCCGCCTGGAACCGATTGTTTTTAAAGATGAAACGCAGCAACTTCCCCTGACGCACAGAACAACGTCCTGTACCGACATTGAGCAGTTa                                                             >  1:332484/1‑217 (MQ=255)                                                                                                                                                                     gACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAAGCGATGATTTCGc                          <  1:117219/249‑1 (MQ=255)                                                                                                                                                                      aCTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCg                       <  1:110278/251‑1 (MQ=255)                                                                                                                                                                               cGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTggg              <  1:333442/251‑1 (MQ=255)                                                                                                                                                                               cGCTGTGTCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAAGTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTTTTTAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACGCTGTCGCTCAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGTGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTggn              <  1:39284/251‑2 (MQ=255)                                                                                                                                                                                     gtCTGCTTTTCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTAcc                                                                                                       <  1:96357/156‑1 (MQ=255)                                                                                                                                                                                          cTTTTCCCGACTATTCTTAATAAGCTTCGCTGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGCTCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATNAAANNNNNaannnnnn                                                                                                        >  2:209440/1‑144 (MQ=255)                                                                                                                                                                                            tttCCCGACTATTCTTAATAAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTGACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCgn  <  1:31654/250‑2 (MQ=255)                                                                                                                                                                                                               |                                                                                                                                                                                                                                       GCCTTTTTTGTAGCCAGAGATTTACGCCTTCGCAGAAGGTTAGATTTTCGAAATCTTTACAAAACAGGAGTGGTAAGAGATATATCGGCATTTATCAGATCGTTAGGTGGCTATAAGTCACGCTAAATGACAGGCTGAATCGAATCATAGCCAGAGCATGCCCTGACTTCACCCCGCTGTGTCTGCTTTTCCCGACTATTCTTAATGAGCTTCGATGCAATTCACGATCCCGCAGTGTGATTTGAGGAGTTTTCAATGGAATATAAAGATCCAATGCATGAGCTGTTGAGCAGCCTGGAACAGATTGTTTTTAAAGATGAAACGCAGAAAATTACCCTGACGCACAGAACAACGTCCTGTACCGAAATTGAGCAGTTACGAAAAGGGACAGGATTAAAAATCGATGATTTCGCCCGGGTTTTGGGCGTATCAGTCGC  >  NC_000913/3719223‑3719659

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑