Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,707,947	C→A	intergenic (‑242/+669)	dppA ← / ← proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,707,947	0	C	A	100.0%	49.4 / NA	19	intergenic (‑242/+669)	dppA/proK	dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base A (12/7);  total (12/7)

ACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAAT  >  NC_000913/3707739‑3708185                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               aCCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGGCCGGTCAGGCAAACGTTATGAGGTGGAGATGCAGTAATAATTAACATCAGTGTAAAAGTGAGGATCCTCCATAAAATGCCACTCGTGACCTACAATCTGTCAACAGAATGTGAGAACGTCAATACAGCAAACAGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAACCTTCAAACAGCTATTTGcagcag                                                                                                                                                                                                         >  1:240959/1‑248 (MQ=255)               nttGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGCCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAACTGCCCCTCGTGACCTCCAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGCTTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTc                                                                                                                                                                                        >  1:32833/2‑251 (MQ=255)                                                   tCGGGTCAACGTTATGAGGGGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCa                                                                                                                                                     >  1:9386/1‑250 (MQ=255)                                                                                                          gCGGATGCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTgg                                                                                              >  1:99655/1‑250 (MQ=255)                                                                                                                   ccATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGGGCTACGCCAACATGACCAGAACAAGCAGCTTAATATTTAGCAACATTTGGTGAGTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAg                                                                                     <  1:166166/250‑1 (MQ=255)                                                                                                                      tAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCNNAAACaa                                                                                                                                                                                                                                            >  2:24067/1‑96 (MQ=255)                                                                                                                                       ccTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAANNACTTCANNc                                                                                                                                                                                                                        >  2:26860/1‑99 (MQ=255)                                                                                                                                       ccTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCAAACCGGGATTTACACAAACGGTGAGCCTCCACAAACAAAGATTAAAAAAACTTCCAACAGCTATTTGCAGCAGAGAACTTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCt                                                                 >  1:55480/1‑250 (MQ=255)                                                                                                                                        cTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTa                                                                >  1:99567/1‑250 (MQ=255)                                                                                                                                                            gtgAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGcc                                           <  1:24067/251‑1 (MQ=255)                                                                                                                                                              gAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGa                                                                                                                    <  1:129109/176‑1 (MQ=255)                                                                                                                                                                   ngTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTg                                                                                                                                                                                                               >  1:66873/2‑80 (MQ=255)                                                                                                                                                                   cGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAggn                                       <  1:104542/248‑2 (MQ=255)                                                                                                                                                                      cAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAgg                                                                                                       <  1:272468/181‑1 (MQ=255)                                                                                                                                                                      cAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATg                                             <  1:307925/239‑1 (MQ=255)                                                                                                                                                                        aTACAGCCAACCGGGATTTACACCAACGGTGAGAATCCCCAAACAAAGATTAAAACAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTACAACATGACCAGAACAATCAGCTTAATATTTCGCAACATTTGGTGATTCAGTTTTATGCATTTAATGaaaa                                                                                                                 >  1:153766/1‑169 (MQ=255)                                                                                                                                                                                  ccGGGATTTACACCAACGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTCTTTGCAGCAGAGCAATTTGTGCTACTCCa                                                                                                                                                                                      >  1:326863/1‑90 (MQ=255)                                                                                                                                                                                                 aCGGTGAGAATCCACAAACAAAGATTAAAAAAACTTCAAACAGCTATGTGCAGCAGATAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGAAACATTTGGTGATTAAGTGTGAGGCAGTTAATGAAAAAAATCTGAGGATAAGGTGGATATCCGATAAATTAATGCCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGgccag                                   <  1:342357/222‑1 (MQ=255)                                                                                                                                                                                                      gagaATCCACAAACAAAGATTCAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTCGCAACATTTGGTGATTAAGTTTTATGCATTTCATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAat  >  1:232506/1‑250 (MQ=255)                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               ACCCTTTGCAGTGGGTTGTCGCTGCCTGACCTTTTTTGTTTTTTGCCCGGTCGGGTCAACGTTATGAGGTGGGGATGCCGTACTAATTAACATCAGTGTAACAGTGCGGATCCTCCATAAAATGCCCCTCGTGACCTACAATCTGTCAACAGAATGTGAAAACGTCAATACAGCCAACCGGGATTTACACCAACGGTGAGAATCCACACACAAAGATTAAAAAAACTTCAAACAGCTATTTGCAGCAGAGAAATTTGTGCTACTCCAACATGACCAGAACAATCAGCTTAATATTTAGCAACATTTGGTGATTAAGTTTTATGCATTTAATGAAAAAAATCTGAGGAAAAGGTGGATATCTGAGCGATTAATACCATGAACGCTAAAACGCACAGCGGAAAATGCCAGGGCCAGCCATAAGTAACGCAAAAAAAGATTTAAGCAAAT  >  NC_000913/3707739‑3708185

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑