Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,646,970	G→T	Q224H (CAG→CAT)	gor →	glutathione reductase (NADPH)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,646,970	0	G	T	100.0%	50.9 / NA	20	Q224H (CAG→CAT)	gor	glutathione reductase (NADPH)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (8/12);  total (8/12)

TGATTCTGATGGCTTCTTCGCCCTTCCTGCTTTGCCAGAGCGCGTGGCGGTTGTTGGCGCGGGTTACATCGCCGTTGAGCTGGCGGGCGTGATTAACGGCCTCGGCGCGAAAACGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCAGCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGCCAATGACAACATCAACCTGGAAGCCGCTGGCGTTAAAACTAACGAAAAAGGCTATATCGTCGTCGATAAATATCAAAACACCAATATTGAAGGTATTTACGCGGTGGGCGAT  >  NC_000913/3646763‑3647207                                                                                                                                                                                                                |                                                                                                                                                                                                                                              tGATTCTGATTGCTTCTTCGCCCTTCCTGCTTTGCCAGAGCGCGTGGCGGTTGTTGGCGCGGGTTACATCGCCGTTGAGCTGGCGGGCGTGATTAACGGCCTCGGCGCGAAAACGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGTTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATAcc                                                                                                                                                                                                     <  1:60819/250‑1 (MQ=255)                                           ctttgCGGTTGTTGGCGCGGTTTACATCGCCGTGGAGCTGGCGGGCGTGATTAACGGCCTCGGCGCGAAAACGCATCGGTTTGGGCGTAAACATGCGCCGCTGCGCAGCGTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCTAAGGCCCTCATTTGCACACCAACGCCACCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGAc                                                                                                                                                                                       <  1:51478/218‑1 (MQ=255)                                               gCGGTTGTTGGCGCGGGTTACATCGCCGTTGAGCTGGCGGGCGTGATTAAAGGCCTCGGCGCCAAAACGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCCGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCCTCTGCACACCAACGCCCTCCCGAAAGCGGTAGTGAAAAATACCGGTGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACggg                                                                                                                                                      >  1:317544/1‑250 (MQ=255)                                                 ggTTGTTGGCGCGGGTGACATCGCCGTTGAGCCGCCTGGCTTGATTAACGGCCTCGGCTCTAAACCGCATCTGTTTGTGCGTAAACATGCGCCTCTGCGCAGCTTCGACCCGGTGATCTCCTAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGcac                                                                                                                                                                                                                                         <  1:346440/166‑1 (MQ=255)                                                  gttgttGGCGCGGGTTACATCGCCGTTGAGCTGGCGGGCGTGATTAACGGCCTCGGCGCGAAAACGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGc                                                                                                                                                                 <  1:57196/237‑1 (MQ=255)                                                                  aCATCGCCGTTGAGCTGGCGGGCGTGATTAACGGCCTCGGCGCGAAAACGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCCGTATTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGTAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTTGGc                                                                                                                                    <  1:228598/250‑1 (MQ=255)                                                                               gCTGGCGGGCGTGATTAACGGCCTCGGCGCGAAAACGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCGATTTGTCGCGAGc                                                                                                                      <  1:287504/251‑1 (MQ=255)                                                                                   gcgggcgTGATTAATGGCCTGGGGGCGAAAACGCATCTGTTGGTGCGTAAACAGGCGCCGCGGCGCAGCTTCGACCCGATGATTCCCGAAACGCTGGTCGAAGGGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGTTAGGGAAAAATACCGAGGGGAGCCTGACGCGGGAGCTGGAAGATGGTCGCAGTGAAACGGTGAATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGc                                                                                                                  <  1:138297/251‑1 (MQ=255)                                                                                   gcgggcgTGATTAACGGCCTCGGCGCGAAAACGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTg                                                                                                                                       <  1:118009/230‑1 (MQ=255)                                                                                        cgTGATTAACGGCCTCGGCGCGAAAACGCATCTGTTTGTGCGTAAACATGCGCCGATGCGCAGCTTCGACCCGATGATTTCCGAAACGATGGTCGAAGTGATGGACGCCGAAGGCCAGCATCTGCACACCAACGCCATCCCGACAGCGGTAGTGAAAAATACCGCTGGTAGCCTGATGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGGGGATTGCCTGATTTGGGCGATTGGTCGCGCGCCTGCCAATg                                                                                                             >  1:53769/1‑251 (MQ=255)                                                                                         gTGATTAACGGCCTCGGCGCGAAAAAGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGCCAATg                                                                                                             >  1:94848/1‑250 (MQ=255)                                                                                                      ccgtcgcgAAAACGCATCTGGTTGTGCGTAAACATGCTCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGGCGCAGTGAAACGGTGTATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGCCAATGACAACATCAACCTg                                                                                               <  1:260996/247‑1 (MQ=255)                                                                                                          cgcgAAAACGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATAGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGCCAATGACAACATCAAc                                                                                                  >  1:147278/1‑244 (MQ=255)                                                                                                                                         gcgcCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGACCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGCCAATGACAACATCAACCTGGAAGCCGCTGGCGTTAAAACTAACGAAAAAGGCt                                                             >  1:93437/1‑250 (MQ=255)                                                                                                                                                  cgcAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGNNGTAGTGnna                                                                                                                                                                                                           >  2:6123/1‑99 (MQ=255)                                                                                                                                                  cgcAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGCCAATGACAACATCAACCTGGAAg                                                                                           <  1:6123/211‑1 (MQ=255)                                                                                                                                                    cAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCg                                                                                                                                   <  1:204939/169‑1 (MQ=255)                                                                                                                                                                gatgatTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGCCAATGACAACATCAACCTGGAAGCCGCTGGCGTTAAAACTAACGAAAAAGGCTATATCGTCGTCGATAAATATCaa                                      >  1:241593/1‑250 (MQ=255)                                                                                                                                                                                                 ggctgAAGGCCTGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATTCCGATGGTAGCCTGATGCTGGAGGTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGGCAATGACAACATCAACCTGGACGCCGCTGGCGTTAAAACTAACGAAAAAGGCTATATCGTCGTCGATAAATATCAAAACACCAATATTGAAGGTATTTACGCGGTGGGCg    <  1:54313/247‑1 (MQ=255)                                                                                                                                                                                                   ccGAAGGCCCGCATCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAACAATACCGATGGTAGCCTGACGCTGGAGCTGGCAGATGGTCGCAGTGACACGGTGGATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGCCAATGACAACATCAACCTGGAAGCCGCTGGCGTTAAAACTAACGAAAAAGGCTATATCGTCGTCGATAAATATCAAAACACCAATATTGAAGGTATTTACGCGGTGGGCGAt  >  1:307421/1‑251 (MQ=255)                                                                                                                                                                                                                |                                                                                                                                                                                                                                              TGATTCTGATGGCTTCTTCGCCCTTCCTGCTTTGCCAGAGCGCGTGGCGGTTGTTGGCGCGGGTTACATCGCCGTTGAGCTGGCGGGCGTGATTAACGGCCTCGGCGCGAAAACGCATCTGTTTGTGCGTAAACATGCGCCGCTGCGCAGCTTCGACCCGATGATTTCCGAAACGCTGGTCGAAGTGATGAACGCCGAAGGCCCGCAGCTGCACACCAACGCCATCCCGAAAGCGGTAGTGAAAAATACCGATGGTAGCCTGACGCTGGAGCTGGAAGATGGTCGCAGTGAAACGGTGGATTGCCTGATTTGGGCGATTGGTCGCGAGCCTGCCAATGACAACATCAACCTGGAAGCCGCTGGCGTTAAAACTAACGAAAAAGGCTATATCGTCGTCGATAAATATCAAAACACCAATATTGAAGGTATTTACGCGGTGGGCGAT  >  NC_000913/3646763‑3647207

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑