Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,507,249	C→T	D30N (GAC→AAC)	yhfT ←	uncharacterized protein YhfT

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,507,249	0	C	T	100.0%	45.9 / NA	17	D30N (GAC→AAC)	yhfT	uncharacterized protein YhfT
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (10/7);  total (10/7)

AGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGC  >  NC_000913/3507011‑3507476                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    aGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCATTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTGGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAgc                                                                                                                                                                                                                         <  1:179759/251‑1 (MQ=255)          acGCCCCAAAGAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGGGTATATCGGTAGGAAGAAAGAGTACCCATGCGTTGAGCAGCCCGGTGTTCTGGGGAAAAGAGATCCCCACCGAGTCCACAAACCCATTGCTCAGAGCTAAATCGAGACTCCTCGCCTGGGGACGGTGCA‑ATAATCTTTCAATCAGTTGCGGAAGGAGCGGGTGGAGGCCGTTATGAAAACCAGCCGCCGAGCg                                                                                                                                                                                                                <  1:132345/251‑1 (MQ=255)                       cgcCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGACGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAg                                                                                                                                                                                                                          >  1:232371/1‑228 (MQ=255)                                                       gCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCTATACTCCCCGCCTCGCGACTGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTc                                                                                                                                                                                             <  1:159171/225‑1 (MQ=255)                                                                ncGAGGATATCGGTAGGAAGAAAGAGCAACCATACGTTGAGCAGCCCGGTTTTCAGGGTAAAAGGGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGCTACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAAAAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATAACCGTCAGGCACGCCACCACGATAATcataat                                                                                                                                                          >  1:210319/2‑246 (MQ=255)                                                                ccGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTAAGGGTAAAAGAGATCCCCACCGAGGCCAAAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAat                                                                                                                                                          >  1:179218/1‑251 (MQ=255)                                                                 cGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAat                                                                                                                                                          >  1:176326/1‑250 (MQ=255)                                                                               ngaagaaAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCACCACCGAGGCAACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGAGGCAGGATAGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAatatcc                                                                                                                                                      >  1:69402/2‑238 (MQ=255)                                                                                   gaaagaGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTg                                                                                                                                       <  1:182576/251‑1 (MQ=255)                                                                                                        gcagcCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATAc                                                                                                                                                                                         <  1:14709/180‑1 (MQ=255)                                                                                                             ccGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTTATGa                                                                                                                                                                                                                                 <  1:12790/135‑1 (MQ=255)                                                                                                                                                      aCCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGCTGCGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCaa                                                                     >  1:205932/1‑250 (MQ=255)                                                                                                                                                                            aTACTCCCCGCCTCGCGACGGGTAATAAA‑GCCTTCCATCAGTTGCGGCAGGATCGGGAGGATGCCGTTATGAAAAACCGCCGCCGAGCGATGCGCCAGAAGCGCGGTCATACCCGTCAGGCACGCCACAACGCTAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGTAAAGCCAAACGCCACTTTTCCTTCAGCGATc                                              >  1:109218/1‑251 (MQ=255)                                                                                                                                                                                    cGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCCGATGCCGTTATGAAAAACAGCCGCCGGGCGATGCGCCAGAAGCGCTGTAATACCCGTCAGGCACGCCACCACGATAATCTCAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAAc                                                                   >  1:345722/1‑222 (MQ=255)                                                                                                                                                                                                                         cGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATCCCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGCCGTGCTCAACGGAAAgg                                                                         >  1:307328/1‑180 (MQ=255)                                                                                                                                                                                                                         cGGCAGGATCGGGCGGATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGc  <  1:282699/251‑1 (MQ=255)                                                                                                                                                                                                                                        gATGCCGTTATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCa                                                                                                                                               >  1:74991/1‑95 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                    AGGATCAACACGCCCCAGATAGCGCCAAGACCAAACGCCATCAGGCTGTTTATCGCCAGGACGCCGAGGATATCGGTAGGAAGAAAGAGTAACCATGCGTTGAGCAGCCCGGTTTTCAGGGTAAAAGAGATCCCCACCGAGGCCACAAAACCAATGCTCAGACCAAAAGCGATACTCCCCGCCTCGCGACGGTTCATATA‑GCCTTCAATCAGTTGCGGCAGGATCGGGCGGATGCCGTCATGAAAAACAGCCGCCGAGCGATGCGCCAGAAGCGATGTCATACCCGTCAGGCACGCCACCACGATAATCTGAATATACAGATCCATAGTCGTGCCTTTTATTTCAGATGGTTAATCAGCATCGGAATCGCGTGTTCGACGTGCTCAACGGAAAGGCCAAACGCCACTTTTCCTTCAGCGATCATTTTGGCGATATGCTCGTCTTTCGCTTTAATGCCTGGTTTGGC  >  NC_000913/3507011‑3507476

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑