Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	395,169	C→T	E373E (GAG→GAA)	ampH ←	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	395,169	0	C	T	100.0%	29.8 / NA	13	E373E (GAG→GAA)	ampH	peptidoglycan DD‑carboxypeptidase/peptidoglycan DD‑endopeptidase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/6);  total (7/6)

GAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCA‑AACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGA‑CCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAAC  >  NC_000913/394923‑395412                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     gAACAAATACGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTAGCTGCTTTGCGTAAAGGCGGCTCTATCGAAATGAATAAAGGCA‑AACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGTTCgg                                                                                                                                                                                                                                                  >  1:294285/1‑251 (MQ=255)                                                               tGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCA‑AACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGTTCGGTCACCAGGTCAGTGATGCCATCGCTCATATTGTTAAAGCTCGTCAACGGCGAACGAGTGa‑c                                                                                                                                                                                    <  1:95918/250‑1 (MQ=255)                                                                       tgaATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGTCTATATCGAAATGAATAAAAGCA‑AACTGGTCGCTATCAACCGTGTGCCTTCAGAGTGTTAATCAGGACGCGTTGATAACCAACGGTTTATTCCCGCTTAGTTCGGTCACCAGGTCATTGATGcc                                                                                                                                                                                                                               <  1:190389/200‑1 (MQ=255)                                                                                                           cTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGACATGCATAAAG‑CACAACTGGTCGCTATACACCGTTTACCTTCAGAGTATTAATCAGAACGAGGGGCTAACCACCGGCTTATTCCCGCTTAGTTCGGTCACCAGGTCATAGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGA‑CCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGACatat                                                                                                                                        >  1:341241/1‑250 (MQ=255)                                                                                                                gtcgCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCA‑CACTGGTCGCTATCAACCGTTTGCCTTAAGCGTATTCATCAGGAAGCGGGGATAACCACCGGTTTATTCCCGCTTAGTTCGGTAACCAGGTCATTGATGCCATCGCTCATATTTTTAACGCGCGTCAACGGCGAACGAGTGA‑CCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTa                                                                                                                                   >  1:325537/1‑250 (MQ=255)                                                                                                                            ntcGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCA‑AACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGTTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGA‑CCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGccac                                                                                                                      >  1:90693/2‑251 (MQ=255)                                                                                                                                    tttGCGTAAAGGCGGCTATATCGAAATGAATAAAGTCA‑AACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAAGCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGTTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGATTTA‑CCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAAc                                                                                                                            <  1:256791/237‑1 (MQ=255)                                                                                                                                                               gAATAAAGGCA‑AACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCACGCTTAGTTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGA‑CCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCatat                                                                                                                                        >  1:203458/1‑198 (MQ=255)                                                                                                                                                                                                 gCCTTCAGAGTATTAATCAGGACGCGGGGATAACCACCGGTTTATTCCCGCTTAGTTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGA‑CCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAAc                                                    >  1:301458/1‑249 (MQ=255)                                                                                                                                                                                                 gCCTTCAGAGTATTAATCAGGACGAGGGGATAACCAACGGTTTATTCCCGCTTAGTTCGGTCACCAGGTCATTGCTGCCATCGCTCATATTTTTACAGCGCGTCAACGGCGAACGAGTGA‑CCACCACACACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCCTATATACCCAACCTAAAc                                                    >  1:301456/1‑249 (MQ=255)                                                                                                                                                                                                                aacAGGACGGGGGGATACCCAACGTTTTATCCCCGTTTAGTTCGGTCCCCAGGTCTTTGATGCCACCGCTCATATGTTAAAAGCGCGTCAACGGCGACCGAGTGC‑CCACCAAAAACGCGCAAATAGTCTTCGGCGGGATCACCGCGATAGAGGTAATAAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCtttn                                   <  1:36244/249‑2 (MQ=255)                                                                                                                                                                                                                                 cccAACGGTTTTTTCCCGCTTAGTTCGGTCCCCAGGACATTGATGCCATCCCTAATATTTTAAAAGCGCTCCAACGGCAAACGA‑TGAACCACCAAAAACGCCCCAAGATTCTTTTGGGTGATCGTCGCCAAATAGTTTAAGAAACCGCCCCCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGcc                  <  1:192390/250‑1 (MQ=255)                                                                                                                                                                                                                                                 cgtttAGTTCGGCCACCAGTTCTTTGATCCCATCGCTCATTTTTTTAAAGCGCGTAAACGGCGAACGGGTGA‑CCCCCACAACCGCCCAAATATTCTTCGGGGGGATCACCGCAATATAGGTAATAAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAAc  <  1:198950/248‑1 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                     GAACAAATCCGCGCAACATTATTATCAATGATTGACTGGAATGAAGAATTGCGATCACGTATTGGTGTGATGAATTATATCCATCAACGTACACGCATATCGCGTTCTGTCGTCGCAGAAGTTCTCGCTGCTTTGCGTAAAGGCGGCTATATCGAAATGAATAAAGGCA‑AACTGGTCGCTATCAACCGTTTGCCTTCAGAGTATTAATCAGGACGCGGGGATAACCAACGGTTTATTCCCGCTTAGCTCGGTCACCAGGTCATTGATGCCATCGCTCATATTTTTAAAGCGCGTCAACGGCGAACGAGTGA‑CCACCACAAACGCGCCAATATTCTTCTGCGGGATCATCGCCATATAGGTAATGAAACCGCCACCACCGCCTGTCTTCTGAATAATCCCCGGACGACCCTCTTTCGGGGCCATATATACCCAACCTAAACCAAGCGCATCCGCTTTGCCGGGGACATCCATGCCAATCACTTTCGTAAAC  >  NC_000913/394923‑395412

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑