| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,489,288 | G→A | A298V (GCG→GTG) | argD ← | N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,489,288 | 0 | G | A | 90.9% | 23.5 / NA | 11 | A298V (GCG→GTG) | argD | N‑acetylornithine aminotransferase/N‑succinyldiaminopimelate aminotransferase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (9/1); minor base C (0/1); total (9/2) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.82e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
CATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGC > NC_000913/3489070‑3489474 | cATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCTCCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCCGCACTTCAGGGGTATTGATGATATCAAACACCGCCCCCGCTACTGCACAGGCCAGAGGAtt > 1:4628/1‑250 (MQ=255) cATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCTCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACACCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAgg > 1:198877/1‑251 (MQ=255) aTCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGAACCATACCGCGAATATCGCTCAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTTGCGTTTCGCGTGAATGACTTCCAGCACTTCAGGGGTATTGATGATATCAAACACCGCCCCAGCAACTACTCAGGCCAGAGGATTACCGCCGTAGGTGGCACCGTGAGAAACAGGATGAAACGCAGatg > 1:225871/1‑249 (MQ=255) cccgcccttgttACTGTGGTTCCAGCTCGGCGCCAATCAACAGCCCCATACCGCAAATATCGCTAAATACAGCGTACTGCTTATCGATCTTCTGCAGATGGTCAACAAATCGCTGTCCTTTCGCCTTAATGCCTTCCAGCACTTCAGGGTTATTTATTAGACCAAACACCGCCCCCGCTACTGCCCAGCCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTcn < 1:35283/240‑2 (MQ=255) ccAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACACAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACACCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGACCCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGACAGCATGGCGCTAATCGGGAAgcc > 1:118665/1‑251 (MQ=255) tACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCTTCTGCATATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGCATTGATGATATCAAACACCGCCCCCGCTACTGCACCGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAAACATGATGAAACGCAGAAGCAATTTCCGCCGAGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTc > 1:162825/1‑251 (MQ=255) cTAAATACATCGTACGGCTGATCGATCTTCTGCAGATGGTAAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATAAAACCCCCCCCCCGCTATTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGTTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGa < 1:330501/249‑1 (MQ=255) ntgctgATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACACCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCACAGAGGTCAGAATATCCGGCGTAACGc > 1:87132/2‑251 (MQ=255) gCCTGAATGCATTCCAGCACTTCAGGGGTATTGATGATATCAAACACCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGGGAGAACCAGGATGAAACGCAGAAg > 1:6756/1‑121 (MQ=255) cAGCACTTCAGGGGTATTGATGATATCAAACACCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATg > 1:202990/1‑130 (MQ=255) nggTATTGATGATATCAAACACCGCCCCCGCTACTGCACAGGACAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGc > 1:68511/2‑207 (MQ=255) | CATTCAGCACCATTACGCCAGCCTCTGCGCCCGCATACAGGAAATCACGCGCCCGACCTTTGTACTGTGGTTTCAGCTCTGCGCCAATCAACAGCCCCATACCGCGAATATCGCTAAATACATCGTACTGCTGATCGATCTTCTGCAGATGGTCAACAAAACGCTGGCGTTTCGCCTGAATGCCTTCCAGCACTTCAGGGGTATTGATGATATCAAACGCCGCCCCCGCTACTGCACAGGCCAGAGGATTACCGCCGTAGGTGGAACCGTGAGAACCAGGATGAAACGCAGAAGCAATTTCCGCCGTGGTCAGCATGGCGCTAATCGGGAAGCCGCCGCCTAACGCTTTCGCAGAGGTCAGAATATCCGGCGTAACGCCGTAGTGCATGTAAGCAAACAAATCGC > NC_000913/3489070‑3489474 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |