| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 3,486,175 | G→A | C19Y (TGC→TAC) | crp → | DNA‑binding transcriptional dual regulator CRP |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 3,486,175 | 0 | G | A | 86.7% | 25.6 / NA | 15 | C19Y (TGC→TAC) | crp | DNA‑binding transcriptional dual regulator CRP |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (8/5); minor base C (2/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.24e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.80e-01 | |||||||||||
ACAGTTGATAGCCCCTTCCCAGGTAGCGGG‑AAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAC > NC_000913/3486015‑3486419 | aCAGTTGATAGCCCCTTCCCAGGTAGCGGG‑AAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCGCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAggg < 1:137742/206‑1 (MQ=255) nccTTCCCAGGTAGCGGG‑AAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCtgtg > 1:85499/2‑237 (MQ=255) cccgtCCCAGGTAGCGGGGATGC‑TATTTCGGCTATCGAGTGACTGGGGCTTTATCTTGCTCGGGAAAAAGCTTTGAACAGATGATAACCTCGCATGTTGCTTTGCAACCCGCAAACATACCCCACTCTCTAATGGTTCTTGTCTCATTACCTCATGCATAAGTTCCCATCCATTAGCACGCTTATTCTCCAGCGTTAAAAAGTGGAAACGCTGTATTTCATTGTTAAAGGCTCTGTGGCAGTGCTGATCaa < 1:310621/247‑1 (MQ=255) cAGGTAGCGGG‑AAGCATATTTCGGCAATCCAGAGACAGCGGAGTTATCTGGCTCTGGCGAAAGATTATACCAGCGGATAAACGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGATCTTGTCTCATTCCCACATTCATa > 1:359274/1‑153 (MQ=255) ggTAGCGGG‑AAGCATCTTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGCTAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGCAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGa > 1:124915/1‑248 (MQ=255) gCATATTTCGGCACTCCAGAGACAGAGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGCGGATACCCGCGCATGGTGCTGGGCAAACCGCACACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACTACATTCATAAGTACACATCCAAGAGCACGCTTATTCACCAGGGTGAACAAGCGGAAACGCTGTACTACATCGTTCAAGGCGCTGTGGCAGTGCTGATCAGAGACGAAGCGGGTAAAGaa > 1:268233/1‑250 (MQ=255) cATATTTCGGCAATCCAGAGACAGCAGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAAGATAACCGCGCATGGTGCTAGGCAAACCGCAAACAGAACCGACTCTCGAAAGGTTCTTGTCTCATTACCACCTTCATAAg > 1:53106/1‑141 (MQ=255) gcgTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGAACCGACTCTCGAATGGTTCTTTTCTCATTACCACATTCATAAGTACCCATCCCAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACTCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTg > 1:171545/1‑251 (MQ=255) ggAGANNGCNNNTANNAGAGGATAACCGCGCATGGTGCTNGGCAAACCGCAAACAGACCCGACTCTAGANTNGNTCTTNNCTCNTTACCACATTNATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGNANCGCTGTACTACATCGTTAAAGGCTCTGTGGCNGTGCTGATCAAAGACGAAGAGGGTNAAGANNTGNtn > 2:257458/1‑213 (MQ=255) aaCAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAAGCCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCAGAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTTATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGTATCAGGGTGATTTTATTTGCGAACTGGGCCTGTTTGa < 1:230089/251‑1 (MQ=255) aCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTCCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGaaa > 1:144122/1‑101 (MQ=255) aCCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTACCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGGTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTg < 1:297938/251‑1 (MQ=255) cAAACAGACCCGCCTCTCGAATGGTTCTTGTCTCATTACCACATTCATACGTACCCATCCAAGCGCACGCTTATTCACCAGGGAGAAAAAGCGGAAACGCTGTACTACATCGTTAACGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAggg > 1:288596/1‑152 (MQ=255) ctcATTACAACATCCATCAGTACCCCTCCAAGAGCACGCATATTCACCAGGGCGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGCGCTGATCAAAGACGACGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAc > 1:291655/1‑251 (MQ=255) cncANTACCANATTCNTAAGTACCCATCCAANAGCACGCTGAATCACCAGGNNGNAAAAGNGGaaa < 2:144122/66‑1 (MQ=255) | ACAGTTGATAGCCCCTTCCCAGGTAGCGGG‑AAGCATATTTCGGCAATCCAGAGACAGCGGCGTTATCTGGCTCTGGAGAAAGCTTATAACAGAGGATAACCGCGCATGGTGCTTGGCAAACCGCAAACAGACCCGACTCTCGAATGGTTCTTGTCTCATTGCCACATTCATAAGTACCCATCCAAGAGCACGCTTATTCACCAGGGTGAAAAAGCGGAAACGCTGTACTACATCGTTAAAGGCTCTGTGGCAGTGCTGATCAAAGACGAAGAGGGTAAAGAAATGATCCTCTCCTATCTGAATCAGGGTGATTTTATTGGCGAACTGGGCCTGTTTGAAGAGGGCCAGGAACGTAGCGCATGGGTACGTGCGAAAACCGCCTGTGAAGTGGCTGAAATTTCGTAC > NC_000913/3486015‑3486419 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |