Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,474,425	T→G	K43T (AAA→ACA)	rpsL ←	30S ribosomal subunit protein S12

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,474,425	0	T	G	100.0%	35.2 / NA	12	K43T (AAA→ACA)	rpsL	30S ribosomal subunit protein S12
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (10/2);  total (10/2)

CAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAAT  >  NC_000913/3474242‑3474660                                                                                                                                                                                        |                                                                                                                                                                                                                                            cAGTCAAGCGCACCACGTACGGAGTGGTAACGAACACCCGGGAGGTCTTTCACAGGCCGGCCACGGATCAGGGTAACGGAGTGCTACTGCAGGTTGTGACCTTCACGACCGATGTAGGAAATCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGCGGGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCAc                                                                                                                                                                          >  1:343494/1‑251 (MQ=255)   ntCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGCGTGGTAGTATATACACGAGTACATACGCCACGTGTTTGCggg                                                                                                                                                                                              >  1:214764/2‑229 (MQ=255)                           gTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGCTCAGGATCACGGAGTGCTCCTGCAGGCTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGACCACGGCATACTTTACGCAGAGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGtt                                                                                                                                                                       >  1:355980/1‑228 (MQ=255)                                           aGGTCTTTAACACGACCGCCACGGATCAGGATGACGGAATGCTCCTGCGGGTAGTGCCCAACACCACCGATGCAGGAAGTCACTTCGAAACCGTTAGTCCGAAGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTAcc                                                                                                                                  >  1:298504/1‑249 (MQ=255)                                            ggTCTTTCNCNNNANNGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTNACCACCGATGGAGGANGTNACTTNGAANCCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGTATg                                                                                                                                                                                          >  2:242755/1‑192 (MQ=255)                                               cTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTgg                                                                                                                                            <  1:23039/235‑1 (MQ=255)                                                     nacGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACt                                                                                                                      >  1:217745/2‑251 (MQ=255)                                                                                      nntGNNNNNTGTNACCTTCACCACCGATGTAGGAAGTCACTGCGAAACCGTTAGTCATGCGAACACGGCATGCGTTACGCAGCGCGGAGTTCGGTTTGGTAGGTGTGGTAGTATATACGCGAGTACATACGCCACGTTTGTGCggg                                                                                                                                                                                              <  2:214764/144‑1 (MQ=255)                                                                                                          ccaccGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGtaata                                                                  >  1:97408/1‑250 (MQ=255)                                                                                                                                 gAAACCGTTAGTCAGACGAACACGGCATACTTTACGCCGCGCGGAGTTCGGTTTTGTAGGCGTGGTAGaatata                                                                                                                                                                                                                           >  1:338260/1‑74 (MQ=255)                                                                                                                                                         gCATACTCTACGCAGCGCGGAGTTCGGTTTTGTAGGAGAGGTAGTATATACACGAGTACATACGACAAGTTTTTGCGGGAATGCTTCCAgc                                                                                                                                                                                  >  1:238638/1‑91 (MQ=255)                                                                                                                                                                          cgGAGTTCGGTTTTGTAGGAGTGGTAGTATATACACGAGTCCATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCACCTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGGGTCAAGacat  >  1:224520/1‑247 (MQ=255)                                                                                                                                                                                        |                                                                                                                                                                                                                                            CAGTCAAGCGCACCACGTACGGTGTGGTAACGAACACCCGGGAGGTCTTTAACACGACCGCCACGGATCAGGATCACGGAGTGCTCCTGCAGGTTGTGACCTTCACCACCGATGTAGGAAGTCACTTCGAAACCGTTAGTCAGACGAACACGGCATACTTTACGCAGCGCGGAGTTCGGTTTTTTAGGAGTGGTAGTATATACACGAGTACATACGCCACGTTTTTGCGGGCATGCTTCCAGCGCAGGCACGTTGCTTTTCGCAACTTTGCGAGCACGTGGTTTGCGTACCAGCTGGTTAACTGTTGCCATTAAATAGCTCCTGGTTTTAGCTTTTGCTTCGTAAACACGTAATAAAACGTCCTCACACAATATGAGGACGCCGAATTTTAGGGCGATGCCGAAAAGGTGTCAAGAAAT  >  NC_000913/3474242‑3474660

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑