Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,391,418	C→T	G204D (GGC→GAC)	tldD ←	metalloprotease subunit TldD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,391,418	0	C	T	100.0%	41.4 / NA	17	G204D (GGC→GAC)	tldD	metalloprotease subunit TldD
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (7/10);  total (7/10)

CAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGC  >  NC_000913/3391222‑3391596                                                                                                                                                                                                     |                                                                                                                                                                                   cAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCGGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGTCGCCACTGGCACcg                                                                                                                                                                      >  1:12423/1‑211 (MQ=255)    aCCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCAAACCTGCGCCACGTACTACCGGCATGGTGCCCGCTGGAGCAGCAACGGCAGAAAGATTGACTAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGAAAGCATTCATAACCAAACCGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCCTCTTCTTCGACGAGAACGCtaccggaa                                                                                                                            >  1:238512/1‑243 (MQ=255)                     cATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACAGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACAGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGc                                                                                                                                    >  1:238731/1‑225 (MQ=255)                                     cGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCAGACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACg                                                                                                                               <  1:148186/214‑1 (MQ=255)                                                  ccAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCGCACGGAAAGACGCACCAGCTGACTGATATCCGCCGCTAGCGTGCCGTCGGTGGc                                                                             <  1:300279/251‑1 (MQ=255)                                                                          gCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATCCGCGCTGCTTCTTTTCCCCATGCGTCCGCACTGACTTCGCCGTCGAGGTCGGCAAGGAAGAATTCATAACCAAAACGCCCGCCGTCTGCACTGGCACCGCGTTCGCCTGTTCCATCTTCTTCGGCGAGAACGTTCACGCAAGGGCGCACCATGGGACGGACAAGCGCCGCGAGCGTGCCGTTGATGACCGCAACCAAAAGTAATTCATAGac                                                     <  1:348745/251‑1 (MQ=255)                                                                          gCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATACATAACCAAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGTGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGACCGCAACCAAAATTAATTCATAGac                                                     <  1:348731/251‑1 (MQ=255)                                                                              gTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAAc                                                                       >  1:196368/1‑229 (MQ=255)                                                                                        ggCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATACGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCCAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGAGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTgg                                       >  1:244674/1‑251 (MQ=255)                                                                                                         gcgcCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTAcg                      <  1:281696/251‑1 (MQ=255)                                                                                                                 cgcccggCTTCTTGGGCCCATGCATCCGCACGGACTGCGCCGTCGAGACCGGCAAGGAAGAATTCATAACCAAAACGCCCGCCGTAGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAAACACCACGGAggn                                          <  1:86282/217‑2 (MQ=255)                                                                                                                              ttgcccagtcttcctcACGGACTTCGCCGACTAGCTCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGTCCCCACTGTCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCTGCGTACGGACATCCGCCGCTATCGTGCTTTCGGTGTTCGCATCCAAAATTAATTCATAGACTCCACTGAGGCTGGCAGTCCCTTCCTGTACGCGCTTGTCCGCTTCGCGGGc  <  1:250781/235‑1 (MQ=255)                                                                                                                                             cACGGACTTCGNCGTNAAGANCGNCAAGGAATAATCCATNACCAAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCNNTNNNCNTCGACgag                                                                                                                                         <  2:252126/100‑1 (MQ=255)                                                                                                                                             cACGGACTTCGCCGTCGAGATCGGCANGGAAGANNTCNTAACCAAAACGACCGCCGTNGCNNNTNNNANCGCGTNCGNNTTTGCCATCNTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCNAGCGTGCCGNNGGTGGc                                                                             >  2:79699/1‑160 (MQ=255)                                                                                                                                             cACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGc                                                                             <  1:79699/160‑1 (MQ=255)                                                                                                                                                                               ttCATAACCAAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCNNCggacgnnc                                                                                                        >  2:15897/1‑99 (MQ=255)                                                                                                                                                                               ttCATAACCAAAACGACCGCCGTCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGc                                                                                       <  1:15897/116‑1 (MQ=255)                                                                                                                                                                                                     |                                                                                                                                                                                   CAGACCGTGACCAACCGCTTCATGCAACAGCACGCCCGGCCAACCTGCGCCAAGTACTACCGGCATGGTGCCCGCTGGTGCAGCAACGGCAGAAAGATTGACCAGCGCCATACGCACTGCTTCTTTTGCCCATGCATCCGCACGGACTTCGCCGTCGAGATCGGCAAGGAAGAATTCATAACCAAAACGACCGCCGCCGCCACTGGCACCGCGTTCGCGTTTGCCATCTTCTTCGACGAGAACGCTCACGGAAAGACGCACCAGCGGACGGACATCCGCCGCTAGCGTGCCGTCGGTGGCCGCAACCAAAATTAATTCATAGACACCACTGAGGCTGGCAGTCACTTCCTGTACGCGCTTGTCCGCTTCGCGGGC  >  NC_000913/3391222‑3391596

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑