Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,335,791 G→A T235I (ACC→ATC)  murA ← UDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,335,7910GA95.0% 45.6 / ‑6.1 20T235I (ACC→ATC) murAUDP‑N‑acetylglucosamine 1‑carboxyvinyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (11/8);  minor base C (1/0);  total (12/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

GGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGC  >  NC_000913/3335602‑3335978
                                                                                                                                                                                             |                                                                                                                                                                                           
ggcgCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCAGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAAc                                                                                                                                                >  1:241241/1‑235 (MQ=255)
       tacgtacgTTAACAGCCTTCGGACGTTTGCCATGCATATCAAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGAGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCCCCGCCTAAACGTTCCACAACTTCGATGacgat                                                                                                                          >  1:290892/1‑250 (MQ=255)
                           ggACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGCGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCt                                                                                                     >  1:136860/1‑251 (MQ=255)
                                  tGCCATGCATATCCAGGCTAATCCAGTCTTCACCGACTTCGATGTCCGCTCCAGCGTAACGCAGTTTCGCCAGAACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAATGTACCGCTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTc                                                                                              >  1:343937/1‑251 (MQ=255)
                                   gCCATGCATATCCAGTCTAATCCAGTCTTCTCCGACGTCTATGTCCGCTCCATCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGAGAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTTCCGATTTCGATACTATCCGGCAGAACGCGATAGGCACCGCCGCCTAAACGTTCCACACCTGCGAAGACGATACGATCTGGGCCCTGACCGCTAATTTTc                                                                                              <  1:143521/250‑1 (MQ=255)
                                        gCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTg                                                                                                                <  1:265541/227‑1 (MQ=255)
                                        gCATANNCANNNTANNCAAGTCTTCGCCGACTTCGATGTNCGCTCCAGCGTCACGCAGTTTCGCCAGCANGNCNTCGANNGTANCTGGCTGCGCNTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAANTNCCGATTTCGATACGATCCGGCAGAACGCGATNGACACCGCCGCCTAAACGTTCCACNCCTTNNATNannnnnnn                                                                                                                       >  2:265541/1‑213 (MQ=255)
                                                                                      aGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCGTCGATGACGATACGATCGGTGCCCGGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCAcgcg                                          <  1:248696/251‑1 (MQ=255)
                                                                                        cGTCACGCAGTTTCGCCCGCACGGCGTCGAGAGTATCTGGCTGCGCTTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCTCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACgcgcn                                        <  1:35828/251‑2 (MQ=255)
                                                                                        cGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATAGCCGCCGCCACCAGGAAAGTACCGATTTCGATCCGATCCGGCAGAACGCGGTAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGCTCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGCCGATTTCCGGTTCACGCGCt                                        >  1:342146/1‑251 (MQ=255)
                                                                                             cGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGtt                                   >  1:334028/1‑251 (MQ=255)
                                                                                                aGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTc                                <  1:280720/251‑1 (MQ=255)
                                                                                                       ccAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTACACGTTCCACACCTTCGCTGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATc                         >  1:289722/1‑251 (MQ=255)
                                                                                                          gCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGCTACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTAGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCgtg                      >  1:262476/1‑251 (MQ=255)
                                                                                                            aCGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAGCGCGATAGACACCGCCGCCTAAACGTTCCACACCTGCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGGTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCgtggt                    <  1:354930/251‑1 (MQ=255)
                                                                                                             cNGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCg                        >  1:277472/1‑246 (MQ=255)
                                                                                                              ggCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTGTCGCACCCAGCGTAATCAGGAAGGTCGCGGTATCGACGAGTTCCGGTTCACGCGCTGCGTTTTCAATAATCgtggtgn                  <  1:67889/251‑2 (MQ=255)
                                                                                                                cGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGACGCCGCCACCAGGAAAGTAACGATTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGATCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGGTAACGCGCTGCGTTTTCAATAATCGTGGTGCCt                >  1:238216/1‑251 (MQ=255)
                                                                                                                     agagTATCTGGCTGCGCGTTACGGAAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGATTGCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGGTCCACACCTTCGATGACGATACGAGCGGGGCCCTGACCGCTAAGTTTCGCACCCAGCGTAAGCAGGAAGTTCGCGGTATCGGCGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGc           <  1:142924/251‑1 (MQ=255)
                                                                                                                                cTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTCCCGATTTCGATACGATCCGGACGCACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATACTCGTGGTGCCTTCCGCCAGGGTTgc  >  1:319964/1‑249 (MQ=255)
                                                                                                                                                                                             |                                                                                                                                                                                           
GGCGCGGTACGTACGTTAACAGCCTTCGGACGTTTGCCATGCATATCCAGGCTAATCCAGTCTTCGCCGACTTCGATGTCCGCTCCAGCGTCACGCAGTTTCGCCAGCACGGCGTCGAGAGTATCTGGCTGCGCGTTACGGCAGATAATTTTGCCGCGAGAAATCGCCGCCGCCACCAGGAAAGTACCGGTTTCGATACGATCCGGCAGAACGCGATAGACACCGCCGCCTAAACGTTCCACACCTTCGATGACGATACGATCGGTGCCCTGACCGCTAATTTTCGCACCCAGCGTAATCAGGAAGTTCGCGGTATCGACGATTTCCGGTTCACGCGCTGCGTTTTCAATAATCGTGGTGCCTTCCGCCAGGGTTGC  >  NC_000913/3335602‑3335978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑