Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,329,064	C→T	A34A (GCC→GCT)	dacB →	peptidoglycan DD‑endopeptidase DacB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,329,064	0	C	T	100.0%	49.2 / NA	18	A34A (GCC→GCT)	dacB	peptidoglycan DD‑endopeptidase DacB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (6/12);  total (6/12)

GGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGGTTGTTAGCGCGAGATTATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCCAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCGGCCCCCGCTATTGATTACCACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGTACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTC  >  NC_000913/3328840‑3329302                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               ggTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACTGCTCGATTCCAGGTTGTTATCGCGAGATTATGCGATTTTCCATATTTATCATCGGATTGACCAGCGGTATAGCGTTCAGTGGGCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGa                                                                                                                                                                                                                                   <  1:327674/238‑1 (MQ=255)         aCAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGGTTGTTAGCGCGAGATTAGGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATATCGTTCAGGGTGCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCTCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGTCGGcgcg                                                                                                                                                                                                               <  1:238012/250‑1 (MQ=255)              tCACATTTTGAGAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGGTTGTTAGCGCGAGATTATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCggn                                                                                                                                                                                                          <  1:90438/250‑2 (MQ=255)                           gTCATTTTACCTTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCATTGCGCCGTCGTATGACGGCTCGATTACAGGTTGTTAGCGCGAGATTATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGAGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCAGCCCCCGCTATTGAtt                                                                                                                                                                                            >  1:356350/1‑251 (MQ=255)                           gTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGGTTGTTAGCGCGAGATTATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCGGCCCCCGCTATTGAtt                                                                                                                                                                                            <  1:126695/251‑1 (MQ=255)                                             tCCCGAAGGGTCATCGTTTACTTTATNGGGCGTNNCGNCGTAGTATGACGGCTCGATNCCNNNTNNNTNGCGCGNGANNATGCGATTTNCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCANATGTTGATGNNTACATTACTCAACTCCCCGCTNGTGNNANNCNTg                                                                                                                                                                                                                                         >  2:81498/1‑188 (MQ=255)                                             tCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGGTTGTTAGCGCGAGATTATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTg                                                                                                                                                                                                                                         <  1:81498/188‑1 (MQ=255)                                                                                   cGTAGTATGACGGCTCGATTCCAGGTTGTTAGCGCGAGATTATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCGGCCCCCGCTATTGATTACCATAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTg                                                                                                                                    <  1:61470/251‑1 (MQ=255)                                                                                                        cAGGTTGTTAGCGCGAGATTATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCGGCCCCCGCTATTGATTACCACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCggn                                                                                                               <  1:218441/251‑2 (MQ=255)                                                                                                           gttgttAGCGCGAGATTATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGGCGGCGCGTCGGCCCCCGCTATTGATTACCACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTggn                                                                                                                                  <  1:34159/229‑2 (MQ=255)                                                                                                                                    ttCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACAGTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCGGCCCCCGCTATTGATTACCACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCTCTGGCGGCGTTGATTCAACTCGGCCCCGATTT‑CGTTTTACCACGACGCTTg                                                                                   <  1:47674/250‑1 (MQ=255)                                                                                                                                                           ccAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACAATACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGACGGCACGACGGCCCACGCTATTGATTACCACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAAc                                                            >  1:238447/1‑251 (MQ=255)                                                                                                                                                                 gTATAGCGTTCAGTGTTCAGGCNGNAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCNCTGATGGTGCNAAANNTCGGNGNGNCGGCCCCCGCTATTGATTACCACAGTCAGNAGATGGCGCTGCCTGCCAGTACCNNGANNNTGNNTACTg                                                                                                                                         <  2:259669/168‑1 (MQ=255)                                                                                                                                                                 gTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCGGCCCCCGCTATTGATTACCACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTg                                                                                                                                         >  1:259669/1‑168 (MQ=255)                                                                                                                                                                             gtgtTCAGGCCGTAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATg                                                                                                                                                                                                                                 >  2:14144/1‑68 (MQ=255)                                                                                                                                                                             gtgtTCAGGCCGTAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATg                                                                                                                                                                                                                                 <  1:14144/68‑1 (MQ=255)                                                                                                                                                                                               ttGATGAGTACATTACTCAACTCCCCGCTGGTGCTAACCTTGCCCTGATGGCGCAAAAAGTCGGCGCGTCGGCCCCCGCTATTGATTACCACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGCTTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGTACTTAAGGGCGAccgaaatcgcctgtgtca                         >  1:150259/1‑233 (MQ=255)                                                                                                                                                                                                                     ccccGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCGGCCCCCGCTATGGATTACCACAGTCAGCAGATGGCGCTGCCTGCCAGGACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCTATTTTCTGTTTACCACGACGCTTGAAACCAAAGTCAATGTGGAAAACGGCGTACTTAAGGGTGACTTAGTGgcgc                                <  1:232349/221‑1 (MQ=255)                                                                                                                                                                                                                     ccccGCTGGTGCTAACCTTGCCCTGATGGTGCAAAAACTCGGCGCGTCGGCGCACGCTATTGATTACCACAGTCAGCAGACGGCGCTGCCTGCAAGTACCCCGCACGAGATTACTGCGCTGGCGGCGTTGATTCAAATCGGCCCCGATCTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGTAATTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTc  >  1:347495/1‑251 (MQ=255)                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                               GGTCAGCTACAATTCACATTTTGATAGTCATTTTACCCTGAAGTTCCCGAAGGGTCATCGTTTACTTTATAGGGCGTTGCGCCGTAGTATGACGGCTCGATTCCAGGTTGTTAGCGCGAGATTATGCGATTTTCCAGATTTATCATCGGATTGACCAGCTGTATAGCGTTCAGTGTTCAGGCCGCAAATGTTGATGAGTACATTACTCAACTCCCCGCTGGTGCCAACCTTGCCCTGATGGTGCAAAAAGTCGGCGCGTCGGCCCCCGCTATTGATTACCACAGTCAGCAGATGGCGCTGCCTGCCAGTACCCAGAAAGTGATTACTGCGCTGGCGGCGTTGATTCAACTCGGCCCCGATTTTCGTTTTACCACGACGCTTGAAACCAAAGGCAATGTGGAAAACGGCGTACTTAAGGGTGACTTAGTGGCGCGATTTGGTGCCGATCCGACGTTAAAACGTC  >  NC_000913/3328840‑3329302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑