Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 3,148,900 G→A G335D (GGC→GAC)  gpr → L‑glyceraldehyde 3‑phosphate reductase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,148,9000GA100.0% 64.4 / NA 21G335D (GGC→GAC) gprL‑glyceraldehyde 3‑phosphate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (9/12);  total (9/12)

CGAAAATGCTTACCGAAGCCAACCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGC  >  NC_000913/3148663‑3149129
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     
cGAAAATGCTTACCGAAGCCACCCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCCATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGCGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGa                                                                                                                                                                                                                                    >  1:358520/1‑241 (MQ=255)
               aaGCCAACCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTcc                                                                                                                                                                                                           <  1:115418/251‑1 (MQ=255)
                                       tATTGAATGAAAGGTCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCTCGCGGAGCAACTAGAGGAGAACGTGCAGGCTCTGGATAATCTGACATGTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCTATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACg                                                                                                                                                                                     <  1:243565/249‑1 (MQ=255)
                                        aTTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACggg                                                                                                                                                                                   <  1:124123/250‑1 (MQ=255)
                                                                                naaaTGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGAtt                                                                                                                                                  >  1:217947/2‑243 (MQ=255)
                                                                                       cGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCt                                                                                                                                                                                                                                 >  1:15131/1‑157 (MQ=255)
                                                                                        gTTAAGCTGGTTGCTGAAAGATGATCNCGTGACNNCGNTATTGATTGGTGCCAGCCGNGCNNNGNNNCNCGAGGNGANNGTGAAGGCGNTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCNCCGATGACGNNCTGAATCTGTGGCAGGGGTCCNCCGNNANNANACCNga                                                                                                                                                                                             >  2:74695/1‑191 (MQ=255)
                                                                                        gTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACGAGAGGAGAACGTGCAGGCGCTTAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGAGAAATGAcc                                                                                                                                                                                                <  1:74695/189‑1 (MQ=255)
                                                                                                   tGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGAGTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTGCCGATAAATGACCTGTTAATAACGCGCGCGCGAACTGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGc                                                                                                                        <  1:183078/250‑1 (MQ=255)
                                                                                                                                         nccAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCgcgc                                                                                                                                                                     >  1:63996/2‑167 (MQ=255)
                                                                                                                                              ccGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTTAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGAGAAATGACCTGTGAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTAGGACGAGTCATTCGGTCAAGATAGCCCAGCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACg                                                                            <  1:245765/251‑1 (MQ=255)
                                                                                                                                              ccGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTAAATCTGTGGCAGGCTTCTTCCGATAAATGACCTGTTAATAACTGGCGCGCGAACCGCGCCCGAAACAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACg                                                                            <  1:348589/251‑1 (MQ=255)
                                                                                                                                                      aGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGa                                                                                                                            >  1:204241/1‑195 (MQ=255)
                                                                                                                                                           cTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGCTTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTAAGATAAATGACCTGTTAATAACGGACGCGCGAACCGCGCACGAat                                                                                                                                                               >  1:329279/1‑155 (MQ=255)
                                                                                                                                                                ggagAACGTGCAGGCGCTGAATAATCNGACATTNNGCNCCAAGGAGCTGGCGCAGATNGANNNGNNNANCGCCGNTGNNGAGCTGAATNTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCNCCCGAATCANNTCAGTGATTATGACGAGTCAGNCGGNNANNANAggncan                                                                                                                    >  2:78824/1‑188 (MQ=255)
                                                                                                                                                                    aaCGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTTATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCg                                                      <  1:286942/251‑1 (MQ=255)
                                                                                                                                                                    aaCGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTTCCACAGCAGTTTGTTATCCg                                                      <  1:78824/251‑1 (MQ=255)
                                                                                                                                                                                 nnaannnnNTGANATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGAtt                                                                                                                                                  <  2:217947/144‑1 (MQ=255)
                                                                                                                                                                                                    cACCAAGGAGCTGGCGCAGATTGCTCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGt                      >  1:129668/1‑251 (MQ=255)
                                                                                                                                                                                                         aGGAGCTGGCGCAGAGTGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTGCGCATCCAGAAAGACGCGCAGTAAGTg                 <  1:296064/251‑1 (MQ=255)
                                                                                                                                                                                                               tGGCGCAGATTGATAAGCATATCGAAGATGACGAGCTGAATCTGTGGCAAGCGTATTCCGAAACATGACCTGTTAATAACGGGCGCGAGAACCGCGCCCGAATAATATCAGTGAATATGACGAGTCAGTAGGGAGAGATAGCCCATCCCAAATTCAGAAAGCACAAACGTCAGATGGATAATGCCTTACAAACTCAGATTGTTATCAGGGAAATTTTTTGCATCCATAAAGACGTGCAATAAGTGaaggg            >  1:257615/1‑247 (MQ=255)
                                                                                                                                                                                                                         tGATCAGCATATCGCCGATGACGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTgn  <  1:85227/250‑2 (MQ=255)
                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                     
CGAAAATGCTTACCGAAGCCAACCTCAACAGCCTGCGCTTATTGAATGAAATGGCACAGCAGCGTGGACAATCAATGGCGCAAATGGCGTTAAGCTGGTTGCTGAAAGATGATCGCGTGACGTCGGTATTGATTGGTGCCAGCCGCGCGGAGCAACTAGAGGAGAACGTGCAGGCGCTGAATAATCTGACATTTAGCACCAAGGAGCTGGCGCAGATTGATCAGCATATCGCCGATGGCGAGCTGAATCTGTGGCAGGCGTCTTCCGATAAATGACCTGTTAATAACGGGCGCGCGAACCGCGCCCGAATCAGATCAGTGATTATGACGAGTCAGTCGGTCAAGATAGCCCATCACAAATGCAGAAAGCACAAACGTCAGATGGATAATGACGTACCACATCAGTTTGTTATCCGGGACATTTTTCGCATCCATAAAGACGCGCAGTAAGTGAATGGAAGAAATTGC  >  NC_000913/3148663‑3149129

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑