Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	3,105,127	G→A	G232E (GGA→GAA)	mltC →	membrane‑bound lytic murein transglycosylase C

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	3,105,127	0	G	A	100.0%	58.4 / NA	22	G232E (GGA→GAA)	mltC	membrane‑bound lytic murein transglycosylase C
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/10);  total (12/10)

TCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCG  >  NC_000913/3104893‑3105302                                                                                                                                                                                                                                           |                                                                                                                                                                                tCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATTGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGTTCCGCCAGGCGTCACGGAAAGATGGCGTTGATGAGTCGCTGATTCTGGGAATGATGCAGACCGAATCTTCCGTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAggtggt                                                                                                                                                                  <  1:56740/250‑1 (MQ=255)              gctgAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAAGTATGCAGACCGAATCTTCCTTGAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCg                                                                                                                                                    <  1:337426/251‑1 (MQ=255)                                  nccGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATTCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCg                                                                                                                                >  1:89904/2‑251 (MQ=255)                                                  gCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTACGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGGTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATAATGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATGCGGc                                                                                                                 >  1:185227/1‑250 (MQ=255)                                                   cGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGGGCTCCGCTCGCAGGGGAAAGCCGGCAc                                                                                                               <  1:99600/251‑1 (MQ=255)                                                                 cGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGGGCGCACAAATATCTCGGCATGATCAGCCAGGCGTCAGGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGtt                                                                                                 >  1:235520/1‑251 (MQ=255)                                                                                                    aaCGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGTGGAAATCCGTCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCgac                                                              <  1:45751/251‑3 (MQ=255)                                                                                                            cacaAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTTTGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGt                                                       >  1:2962/1‑250 (MQ=255)                                                                                                                                  nccAGGCGTCACGGAAATATGGCGTTGCTGAGTCGCTGATTCTGGCAATTATGCAGAACGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGtt                                >  1:33738/2‑251 (MQ=255)                                                                                                                                   ncAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGttt                               >  1:101658/2‑251 (MQ=255)                                                                                                                                        cGTCACGGAAATATGGCGTTGATGAGACGCTGATTCTGGCTCTTATGCAGACCGCATCTTCCTTTAACCCGTATGCGGCCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTacaac                                                                                                                                                             >  1:131361/1‑120 (MQ=255)                                                                                                                                           cACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTaa                                                                                                                                                                            >  1:347172/1‑102 (MQ=255)                                                                                                                                                              tgattCGCTGATTCTGGCAATTATGCAGGCCGAATCTTCCTTTAACCCTCATGCGGTCAGCTGTTCCGATGCTCTGTAATGACTGCAGGTGGTACATCATACTGCCGTTAAAGATGTCTTCTGCGCTCTGGGTAAATCCGGCACGCCGAGCCGCAGTCTCTTGTTTTATCCTGCCAGCAATATTGATACCGGCACcgc                                                         <  1:360243/194‑1 (MQ=255)                                                                                                                                                                aGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGGATCTGGCGATGCTGAACAATGTTTATCGCGGCGGAATTGATAACCCAACATcg  <  1:150317/251‑1 (MQ=255)                                                                                                                                                                            tGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGc                                                                                 <  1:302418/160‑1 (MQ=255)                                                                                                                                                                              gCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAAc                                      >  1:1952/1‑201 (MQ=255)                                                                                                                                                                              gCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGGCAGCCGTTCCGATGCACTTGAATTAATGCAggtg                                                                                                                                                                    <  1:278762/75‑1 (MQ=255)                                                                                                                                                                                    aTGCAGACNGNNNCNNCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTANTGCAGGTGGTACAACNTANTGCCNGGANAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGc                                           >  2:249079/1‑190 (MQ=255)                                                                                                                                                                                    aTGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGc                                           <  1:249079/190‑1 (MQ=255)                                                                                                                                                                                           cnGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGtt                                                                                          >  1:277588/1‑136 (MQ=255)                                                                                                                                                                                                                         gCCGTTCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCGGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATAcn                                                                 <  1:72504/131‑2 (MQ=255)                                                                                                                                                                                                                            nttCCGATGCGCTGGAATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCCCGCCGAGCCGCAGTTTCTTGttt                                                                                         >  1:105074/2‑104 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                TCGCGGATTATCTGCTGAAAAACCGTCTGAAGAGCCGCAGCAACGGGCTGCGTATCATCTACAGCGTCACCATTAACATGGTGCCGAACCACCTTGATAAACGTGCGCACAAATATCTCGGCATGGTCCGCCAGGCGTCACGGAAATATGGCGTTGATGAGTCGCTGATTCTGGCAATTATGCAGACCGAATCTTCCTTTAACCCGTATGCGGTCAGCCGTTCCGATGCGCTGGGATTAATGCAGGTGGTACAACATACTGCCGGGAAAGATGTGTTCCGCTCGCAGGGGAAATCCGGCACGCCGAGCCGCAGTTTCTTGTTTGATCCTGCCAGCAATATTGATACCGGCACCGCGTATCTGGCGATGCTGAACAATGTTTATCTCGGCGGAATTGATAACCCAACATCG  >  NC_000913/3104893‑3105302

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑