Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,774,947	G→A	G544S (GGT→AGT)	yfjW →	uncharacterized protein YfjW

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,774,947	0	G	A	100.0%	20.6 / NA	9	G544S (GGT→AGT)	yfjW	uncharacterized protein YfjW
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (4/5);  total (4/5)

CGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCA  >  NC_000913/2774738‑2775155                                                                                                                                                                                                                  |                                                                                                                                                                                                                 ngTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTCAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCCTTCATTCTTACttt                                                                                                                                                                         >  1:211886/2‑251 (MQ=255)         ccAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTTGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTgn                                                                                                                                                                                                            <  1:41346/208‑2 (MQ=255)                                              aTGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTGTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTTAATGAAAATTTATTCTTGTAACTCATTTGGCtn                                                                                                                            <  1:62104/251‑2 (MQ=255)                                                                                     gCACAGCAGATCTTGTTTTTGTTGATNACAAGANNATNAACCTTCGTAAAATAACTTNTTNNNANNNTNCGCTANAANNTTTTGGTTCNTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCNATAAAATTCNNTCATTCTTACTTTAAGAATGANGTGNNTNNANATTNAtn                                                                                                                                               >  2:77634/1‑192 (MQ=255)                                                                                     gCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCAGTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTc                                                                                                                            <  1:77634/212‑1 (MQ=255)                                                                                                                                  gTAAAAGAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATGTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGAGGTGAATGAAAATTTATTCTTGTAACGCATGTGGCTCCAAGTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGacac                                        <  1:168232/251‑1 (MQ=255)                                                                                                                                                   cAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATCTATTTGGTTTAAGTCTGCTAGCAATTTCTATCACATTCATTCATTCTTACTTTAAGAATGCTGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTAATACCTGCTTTaa                         >  1:352209/1‑249 (MQ=255)                                                                                                                                                              aGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTg            <  1:94771/251‑1 (MQ=255)                                                                                                                                                                                                             gTTTAAGTCTGCTAGCAATTTCTATAAAATTCATTAATTCTTACTTTATGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAACATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCa  >  1:288860/1‑214 (MQ=255)                                                                                                                                                                                                                  |                                                                                                                                                                                                                 CGTTAAGGCCAGAATCGGAGATTATTATTTCTGAAGGGAGTTTGTATGGACTAGTTAATAAAAGCAAAAAAATCAAAATATATGGCACAGCAGATCTTGTTTTTGTTGATAACAAGATAATGAACCTTCGTAAAATAACTTATTTGCAATCTAAGCTAGAAATTTTTGGTTCTTCTATTATGGATATATTAAAGTATATATTTGGTTTAGGTCTGCTAGCAATTTCTATAAAATTCATTCATTCTTACTTTAAGAATGATGTGAATGAAAATTTATTCTTGTAACGCATTTGGCTCCAATTGGAGCCTTTTTATGACTATTTATTAAAATATCCTAAGTTAAGCCTGTTTTTAAAGCAGGCTTGAGCGTGAAGCGGACACTCATACCTGCTTTAAAGTGGCGTTTTTGTCGTTACCCA  >  NC_000913/2774738‑2775155

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑