Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,745,600	A→G	Y107H (TAC→CAC)	rimM ←	ribosome maturation factor RimM

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,745,600	0	A	G	100.0%	44.6 / NA	15	Y107H (TAC→CAC)	rimM	ribosome maturation factor RimM
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (7/8);  total (7/8)

CCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCC  >  NC_000913/2745377‑2745836                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             ccAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATAGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGc                                                                                                                                                                                                                     >  1:227006/1‑249 (MQ=255)    gtaTCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTg                                                                                                                                                                                                                >  1:336320/3‑251 (MQ=255)                                                                     gAACGNNCCNNNAANNTCCTTGATACCAAACGCATCTTTNAGGTTTGCCTTAATGCCGAGAACGTCGTTNGNTNCGGTNNCCANCATATCGACGNCTTTACCGCGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCANGNCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTANGGCAGCTGCGATGATTCCTCGAAANTTTANNAAntnnnnnnnnn                                                                                                                                                                            >  2:254823/1‑213 (MQ=255)                                                                                       cTTGATAACAAACGCATCTTTCTGGATTGCCTTAATGACGAGAACGTCATTAGATCCGGCTTCACTCATATCGACGACTTTACCGACGTCGTAGCATTCAGTGGTTACAACATGGCAGTCAATAAGGTCTTTCCAGCGGTGGTCCCACTCTTCAAGCTGAGGCAGCTGCGATGACTCCACGACCATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGCTCGTCTACGCCTTTCCGCTTGATGCTCATGt                                                                                                                             >  1:244345/1‑251 (MQ=255)                                                                                        ttGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTGACCGAGATCGTAGCCGTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGAt                                                                                                                                                                                                            <  1:226445/171‑1 (MQ=255)                                                                                                       tCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCg                                                                                                                                                                                   <  1:22183/181‑1 (MQ=255)                                                                                                                   gCNNNNNTGANGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGAt                                                                                                                                                                                                            <  2:220505/144‑1 (MQ=255)                                                                                                                                   cGTCATTAGATCCGGTTTCCATCATATCGACGACATTACCGAGATCGTTGCCGTCAGTGGGTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCAGCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGAGTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCAc                                                                                 <  1:325342/251‑1 (MQ=255)                                                                                                                                            aTCCGGTTTCCATCATATCGACGACTTTACCGAGCTCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGCTGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCACAGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGcc                                                                        >  1:143689/1‑251 (MQ=255)                                                                                                                                                              cgacgaCTTTACCGAGATCGTAGCCTTCAGTTGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAggg                                                       <  1:255424/250‑1 (MQ=255)                                                                                                                                                                  gacTTTACCGAGATCGCAGCCTTCACTGGTTACTACCTGGCAGCCCATCAGGACTTTCCAGTGGTAGTACCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGAc                                                                                           >  1:292067/1‑210 (MQ=255)                                                                                                                                                                                               ttACTACCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTcn                                                                                                                                                                                                                                       <  1:70570/41‑2 (MQ=255)                                                                                                                                                                                                  ctacCTGGCAGCCCATCAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGACGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCaa                                                                                                                                                     >  1:334937/1‑120 (MQ=255)                                                                                                                                                                                                    tccTGGCAGGCCATGAGGTCTGTCCAGTGGTATTCCCCCTCTTCAAGCGGAGACAACTGCGATGAAGCCACGAGAATTGCACAATGCGTCAGCAGGTTCCCGGCAGCATGTTCGGCGATGCTCTTCAGCGTGATTATCATGTTCGTATTGGGTTGTGTCCATCTTTCCAGTTGGACTGGGTAGGACTGTGCCGTCTGCGGCATCAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGgaag                 <  1:195384/249‑1 (MQ=255)                                                                                                                                                                                                                  cAGGTCTTTCCAGTGGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCATCTGGACTTTCTGCCACTGACCCGCCTTCTGGATGAGCCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGcc  <  1:132230/251‑1 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                             CCAGGATCCCAATCTACTTCGATTGAACGAGTAGTGAGATCGACTTTCTTGATAACCTGCCCATCGAGGAACGGTACGAGACGTTCCTTGATACCAAACGCATCTTTCAGGTTTGCCTTAATGACGAGAACGTCATTAGATCCGGTTTCCATCATATCGACGACTTTACCGAGATCGTAGCCTTCAGTGGTTACTACCTGGCAGCCCATCAGGTCTTTCCAGTAGTAGTCCCCCTCTTCAAGCTGAGGCAGCTGCGATGAATCCACGACAATTTCACAATTCGTCAGCAGGTTCGCCGCATCACGATCGTCAACGCCTTTCAGCTTGATGATCATGTCCTGATTGTGGTGCTTCCAGCTTTCCAGCTGGACTTGCTGCCACTGACCCGCCTTCTGGATAAACCAGGGCTGATAGTCAAAAATGCTTTCGGCGTCTTCGGTGGAAGAAAACACTCTGAGCC  >  NC_000913/2745377‑2745836

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑