Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 2,710,250 G→A intergenic (‑86/‑170) rseD ← / → nadB rpoE leader peptide/L‑aspartate oxidase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,710,2500GA95.0% 49.3 / NA 20intergenic (‑86/‑170)rseD/nadBrpoE leader peptide/L‑aspartate oxidase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  major base A (11/8);  minor base C (1/0);  total (12/8)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.54e-01

AAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATCCGCACAACATGATGCTATGCTGACCAAACCATGTTTAGTAAATTAAACAAAGAAAATGAATACTCTCCCTGAACATTCATGTGACGTGTTGATTATCGGTAGCGGCG  >  NC_000913/2710021‑2710471
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                             
aaGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTCGCTGCAATTTGCGCAAGACACGGGTTAGAGCGCCTCGTTTTTGTAAAGTTCCGTAAAGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCt                                                                                                                                                                                                           >  1:177895/1‑250 (MQ=255)
  gTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCTAAt                                                                                                                                                                                                        >  1:144863/1‑251 (MQ=255)
      ccccAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCTAAtcctc                                                                                                                                                                                                    >  1:185246/1‑251 (MQ=255)
        ncaaaccaaaTTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTCTAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTAAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCTAAtcctcc                                                                                                                                                                                                   >  1:39138/2‑250 (MQ=255)
         caaaccaaaTTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAATGTCCGTAACGCATCTGTTTTGGTTTGGCATGCGGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGGAGAAATTCCCATTTAACTGCCTGAAGATTAACCCAACAGCCTTTTTATTTCACCACCTAATCCTccac                                                                                                                                                                                                 <  1:227180/251‑1 (MQ=255)
                                cGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTtct                                                                                                                                                                          >  1:299151/1‑251 (MQ=255)
                                 gAAACGACAATCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCATAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGAATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTACCCCAACAGACTTTTTATTTCACCACCTACTCCTCCACCAGCCAGTAACTTCTCTTTTtctc                                                                                                                                                                         >  1:306784/1‑251 (MQ=255)
                                  aaaCGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGTATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcg                                                                                                                                                                        <  1:75060/251‑1 (MQ=255)
                                                                                                                    gttgtttggCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTcgc                                                                                                                                                                       <  1:84478/169‑1 (MQ=255)
                                                                                                                      tgtttgtCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCTAATCCTCCCCCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATCCGCACAACATGATGCTATGCTGan                                                                                     <  1:219722/250‑2 (MQ=255)
                                                                                                                           gtCATGCTGTAGACGGAGCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTAACCACCTACTCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAACTAGCAGGTGTTTATCCGc                                                                                                          >  1:349183/1‑224 (MQ=255)
                                                                                                                                                     atcATTNNAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTcac                                                                                                                                                                                                                <  2:7542/96‑1 (MQ=255)
                                                                                                                                                                    cAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGGGTTTATCCGCACAACATGATGCTATGCTGACCAAACCATTTTTAGTAAATTAAACAAAGAAAATGAATACTCTCCCtn                                      <  1:64855/251‑2 (MQ=255)
                                                                                                                                                                        aTCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGACACCCAACAGCCTTTTTATTTCACCAACTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATCCGCACAACATGATGCTATGCTGACCAAACCATGTTTAGTAAATTAAACAAAGAAAATGAATACTCTCCCTGAAc                                   >  1:195459/1‑250 (MQ=255)
                                                                                                                                                                               cATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCTCCCCCTAATCATACACCAGCCAGTAACTTCTCTTTTTCTCGCCACCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAg                                                                                                                      >  1:207230/1‑160 (MQ=255)
                                                                                                                                                                                 tCGCANTATCTNTNNCGAAATGCTCCTTTAACTGNCTGAAGAGTAACCCANCAGCNTTTTTNTTNc                                                                                                                                                                                                                  >  2:137463/1‑66 (MQ=255)
                                                                                                                                                                                               tAGAAAGGCCCATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCTCCCCCTAATCCTCCTCCTGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCATTTGTTTATCCGCACAACATGATGCTATGCTGACCAAACCATGTTTATTAAATTAAGCAAAGAAAATGAATACTCTCCCTGAACATTCATGTGACGTGTTGATTATc            <  1:267224/250‑1 (MQ=255)
                                                                                                                                                                                                 gAAATGCCCATTTAACTGCCTGAAGAGCAACCCAACCGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCg                                                                                                                                                        >  1:113948/1‑108 (MQ=255)
                                                                                                                                                                                                        ccATTTAACTGCCTGAAGAGTAACCCAACAGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTATCTTTTTCTCGACGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGTAGGTGTTTATCCGCATAACATGATGCTCTGCTGACCAAACCATGTTTAGTAAATTAAACAAAGAAAATGAATACTCTCCCTGAACATTCATGTGACGTGTTGATTATCGGTAgcggcg  >  1:356525/1‑251 (MQ=255)
                                                                                                                                                                                                                           gtatcccAACAGCCTTGTTATTACACCACCGAATCCTCCACCAGCCAGTAACTTCTCtt                                                                                                                                                                               <  1:113203/55‑1 (MQ=255)
                                                                                                                                                                                                                                     |                                                                                                                                                                                                                             
AAGTCTCCCCAAACCAAATTTCCACGCGCTATCGAAACGCCACTCCATTAGCTGCAATTTGAGCAAGCAAAGGGTTAGAGTGTCTCGTTTTTGTAAAGTTCCGTAACGCATCTGTTTTTGTTTGTCATGCTGTAGACGGATCATTATCTATCATTATAAGTCTACAGAATCTGAACATCGCATTATCTGTGTAGAAATGCCCATTTAACTGCCTGAAGAGTAACCCAACGGCCTTTTTATTTCACCACCTAATCCTCCACCAGCCAGTAACTTCTCTTTTTCTCGCCGCCCTGCGTCAGCGTGTTTAGCAACTGTAACAAATATTAAAATAGCAGGTGTTTATCCGCACAACATGATGCTATGCTGACCAAACCATGTTTAGTAAATTAAACAAAGAAAATGAATACTCTCCCTGAACATTCATGTGACGTGTTGATTATCGGTAGCGGCG  >  NC_000913/2710021‑2710471

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑