| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NC_000913 | 309,985 | T→A | G14G (GGA→GGT) | ecpB ← | putative fimbrial chaperone EcpB |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NC_000913 | 309,985 | 0 | T | A | 95.0% | 49.6 / NA | 20 | G14G (GGA→GGT) | ecpB | putative fimbrial chaperone EcpB |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base A (9/10); minor base G (0/1); total (9/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01 | |||||||||||
GATCACTTCGCTGGCTTGGGCGGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCTGGTACATCAGAGAGATTAACTGGTCCAGGTCGCGTCGAACTGTACGCTAACGTCGCCGCTCCAGATGCCTTCCGGTAGAGTGCTGTAATCTGTTACTGCGGTGGTACCATTGGTGGTACCGCTGATGATGGAGAAGGTGAAACCATCC > NC_000913/309748‑310216 | gATCACTTCGCTGGCTTGGGCGGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAAACTGCCCGTCGTTAAACGGAGAAGAGAGCCGTTCGAGACGGATATTGATAAGGAGACAAATGTCGGTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAAcagc > 1:228427/1‑250 (MQ=255) ggCTTGGGCGTGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGTTTGTCCATTGAGATATCCTGACCTGCGTCAATCGGTGAAGAGAGCCGTGCTATACGGATAGTGATAAGGCGACCACTGTGTGTACTGTTTTTGAGCTTGTTGAGCAGCATGCGTCGGTGACTGTGCATAATTGCCTATAGATGGCCGACATTCAGCTCCGGGTGCGCAGATAAACCGGAAAACAGCAGAGCGAGAGGc < 1:131446/250‑1 (MQ=255) ggCTTGGGCGGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAATGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGc < 1:47355/250‑1 (MQ=255) ggCTTGGGCGGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGTCGACCACTGTCGGTACTGTTTTTTATCGGTGTGCTCAGCGTGCTGCTGGCACTGTTCATAAACGATGATATATCTCGGACATCCAGCGCCTGTGCCAGATATATACCGGAAAACAGCAGAGCGAGAGGCa < 1:314525/251‑1 (MQ=255) ncagcaAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGCGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGCTATTGCTCAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCACCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTc > 1:40856/2‑251 (MQ=255) ncagcaAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCtttttt > 1:71038/2‑250 (MQ=255) ncAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCCGGACTaa > 1:212908/2‑250 (MQ=255) cATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATTATATATCTCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGACCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGGCCAGGAATAAAGCAgg < 1:252024/250‑1 (MQ=255) ccTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGAGATTGATAAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCATCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCATCGCCTGGGCCGGAGATATGCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTTCTTTGTCATAATAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCTGGGACATCag < 1:361144/251‑1 (MQ=255) gtcAAGCGGTGAAGAGAGCCGTTAGAGACGGCTATTGATAAGGCGACCACTGTCGGTACTGTTTTTGCTCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCAt > 1:299113/1‑189 (MQ=255) cGGTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCTGGTACATCAGAGAGATTAACTGGTCCAGGTCGCGTCGAACTGTACGCTAACGTCGCCGCTCCAGATGCCTTcc > 1:264098/1‑251 (MQ=255) ggTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCTGGTACATCAGAGAGATTAACTGGTCCAGGTCGCGTCGAACTGTACGCTAACGTCGCCGCTCCAGATGCCTTccn < 1:104847/251‑2 (MQ=255) gTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCTGGTACATCAGAGAGATTAACTGGTCCAGGTcgc > 1:135202/1‑204 (MQ=255) nnnnnnncNATNNTATANCGCCGACATCCAGCGCCTGGGCCGNAGATATACCGGAAAACAGCAGAGCGAGAGGCNGNAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTANCCCTGCTGGTNCATNNGAGANANTNACTGGTCCAGGTCGCGTCGAACTGTACGCNAACGTCGCCGCTCCAGATGCCTTNNGGNNNAGNNCTGTa < 2:264098/213‑1 (MQ=255) cATAAACGATGATATATCGCCGACATCCAGCGCCTGGGACGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCTGGTACATCAGAGAGATTAACTGGTCCAGGTCGCGTCGAACTGTACGCTAACGTCGCCGCTCAAGATGCCTTCCGGTAGAGTGCTGTAATCTGTTACTGCGGTGGTACCATTGGTGGTAc > 1:130986/1‑251 (MQ=255) gCCGACATCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGACTAAAGCAGGGGGGCTACCCCTGCTGGTACATCAGAGAGATTAACTGGTCCAGGTCGCGTCGAACTGTACGCTAACGTCGCCGCTCCAGATGCCTTCCGGTAGAGTGCTGTAATCTGTTACTGCGGTGGTACCATTGGTGGTACCGCTGATGATGGAGAAgg > 1:351285/1‑251 (MQ=255) gACAGCCAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCAGAACAAGTCCGTCCAGGAATAAAGCAGGGGGGTTTCCCCTGCTGGTGCATCAGAGAGATTAACTGGTCCAGGTc < 1:294317/142‑1 (MQ=255) ccAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGTCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCTGGTACATCAGAGAGATTAAc < 1:167244/126‑1 (MQ=255) cAGCGCCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCGGGTACATCAGAGAGATTAACTGGTCCAGGTCGCGGCGAACTGTACGCTAACGTcgc < 1:294281/161‑1 (MQ=255) gcgcCTGGGCCGGAGATATACCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCTGGTACATCAGAGAGATTAACTGGGCCAGGTCGCGTCGAACTGTACGCTAACGTCGCCGCTCCAGATGCCTTCCGGTAGAGTGCGTTAAGCTGTTACTGCGGTGGTAGCATTGGTGGTACCGCTGATGATGGAGAAGGTGAAACCATcc < 1:198447/251‑1 (MQ=255) | GATCACTTCGCTGGCTTGGGCGGGTAGCAGCAAGCTGGCGGGAGTGAGTAGCAACTCATCCGGCTTGTCCATTGAGATAACCTGCCCGTCGTCAAGCGGTGAAGAGAGCCGTTCGAGACGGATATTGATAAGGCGACCACTGTCGGTACTGTTTTTGATCGTTTTGCTCAGCGTGCTGCTGTCACTGTTCATAAACGATGATATATCGCCGACATCCAGCGCCTGGGCCGGAGATATTCCGGAAAACAGCAGAGCGAGAGGCAGAAGGTGCTTTTTCATAACAATTCCGTCCAGGAATAAAGCAGGGGGGCTACCCCTGCTGGTACATCAGAGAGATTAACTGGTCCAGGTCGCGTCGAACTGTACGCTAACGTCGCCGCTCCAGATGCCTTCCGGTAGAGTGCTGTAATCTGTTACTGCGGTGGTACCATTGGTGGTACCGCTGATGATGGAGAAGGTGAAACCATCC > NC_000913/309748‑310216 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |