Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,577,937	T→C	E147E (GAA→GAG)	maeB ←	malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,577,937	0	T	C	100.0%	26.5 / NA	11	E147E (GAA→GAG)	maeB	malate dehydrogenase (oxaloacetate‑decarboxylating) (NADP(+))
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (4/7);  total (4/7)

ACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGC  >  NC_000913/2577758‑2578100                                                                                                                                                                                    |                                                                                                                                                                    aCCAGCAGGTTCATACAGGCTATGGCTGCGGCACCCGCGCCGGAAACCACCAGCCGCACGGCGGAGATGTTTGGCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGGGCTGATAATTGCCGTGCCGTGCTGAGCGTCGTGGAATACCGGAATATGCATCCGCTCGCGCAGTTGCTGCTCAATATAGAAACATTCTGGCGCTTTAAGGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCgcgg                                                                                               <  1:169888/250‑1 (MQ=255)                                  cccGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCt                                                                                                                                    >  1:119050/1‑180 (MQ=255)                                     gcgcCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCGAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCTACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTc                                                          <  1:152412/251‑1 (MQ=255)                                      cgcCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATAGTCATCCGCTCGCGCAGTTGCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATgn                                                                                                                       <  1:68906/189‑2 (MQ=255)                                                caccaTCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGCGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCNCAANNNNNaannnnnn                                                                                                                                                    >  2:211458/1‑144 (MQ=255)                                                                         tnccnncccanGCGCAATCCGTTGAGGATGGCGGCNGTGCTGATAATTGCCGTGCCGTTCTGATCGTNGNGGAATACCGTAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGANACATTCTGGCNCTTNNATGTNTNCNAGGTTGATGCCGCCTAAGGTTGGTTCGAGNGCGGCGACAACTTCAATAAATTTNNCCNNNTCNNGTTCg                                                            <  2:264174/206‑1 (MQ=255)                                                                                     cgcAAGCCGGTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGGCGTGGAATACCGTAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATGCTGGCGCTTTAATGTCTTCGAGGGTGATGCCGCCTAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCCGGTCGAGTTCGTCAACTTCAATg                                                <  1:130999/213‑1 (MQ=255)                                                                                             gTTGAGGATGGCGGTAGTGCTGATAATTGCCGTGCCGCGCTGATCGTAGTGGAATACCCGAATATTCATACGCTCGCGCAGTTTCTGCTCAATATAGACACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCAGCCGAAGGTTGGCTCGAGCGCGGCGACAACTTCACTAAATTTGTCCGGGTAGAGTTCGTCAACTTTCATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGACCGCCCTTGc  >  1:257292/1‑251 (MQ=255)                                                                                             gTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTTGAATACCGGAATATTCATCCGCTCGCGCAGTGTCTGCTCAATATACAAACATGCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGTGTGGTTCGAGCgcggcg                                                                                             <  1:286635/160‑1 (MQ=255)                                                                                                 aGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGGGCGAGcgcgn                                                                                               <  1:102725/154‑2 (MQ=255)                                                                                                                            gtgccgtgcTGCTCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGCTCAATATAGAAACATTCTGGCGCTTTAATGTCTTACCGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAAc                                                                                        >  1:186388/1‑134 (MQ=255)                                                                                                                                                                                    |                                                                                                                                                                    ACCAGCAGGTTCATACAGGCGATTGCTGCGGCACCCGCGCCGGAAACCACCATCCGCACGTCGGAGATGTTTTTCTCCACCACGCGCAAGCCGTTGAGGATGGCGGCAGTGCTGATAATTGCCGTGCCGTGCTGATCGTCGTGGAATACCGGAATATTCATCCGCTCGCGCAGTTTCTGTTCAATATAGAAACATTCTGGCGCTTTAATGTCTTCGAGGTTGATGCCGCCGAAGGTTGGTTCGAGCGCGGCGACAACTTCAATAAATTTGTCCGGGTCGAGTTCGTCAACTTCAATGTCAAATACATCAATCCCGGCGAATTTCTTAAACAGAACGCCCTTGC  >  NC_000913/2577758‑2578100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑