Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,546,804	T→A	D44E (GAT→GAA)	murP →	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,546,804	0	T	A	100.0%	27.7 / NA	11	D44E (GAT→GAA)	murP	N‑acetylmuramic acid‑specific PTS enzyme IICB component/anhydro‑N‑acetylmuramic acid transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (5/6);  total (5/6)

ACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCAT  >  NC_000913/2546560‑2547031                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    aCTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATGCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAat                                                                                                                                                                                                                               <  1:162459/251‑1 (MQ=255)                           cTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGAAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAggg                                                                                                                                                                                                     >  1:300096/1‑251 (MQ=255)                             gAAAAATCTCGATGCCGCATAAGCTAAAAAACGCCTGGATCAACACGGCGGCGTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAggg                                                                                                                                                                                                     <  1:189762/249‑1 (MQ=255)                                 aaTCTCGATGCCGCAGAAGCTAAAACACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGTCCACAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCTGCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCACAACGCTGGAAGGCGTGAAGGGCGTCAt                                                                                                                                                                                               <  1:180597/251‑1 (MQ=255)                                                                                                     cAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGCCCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAg                                                                                                                                                                         >  1:289886/1‑205 (MQ=255)                                                                                                                    ggCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACAGGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCATGTGCAGTTCGTTTTTGGGCCTGGTAAAgcgc                                                                                                                                                <  1:289926/215‑1 (MQ=255)                                                                                                                                                                 tgtCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGCATGACGCGCCTGCGTCTGGGGGTTCATGAAAGTTCACGGGTTGAAACCAATATCACAACGCTGGAAGGCGTGACGGGCGTCATTTTGCCCAGCGACCAGGGGCCGGTCGTTTTTGGGCCTGGTAAAGCGAATCGCGCCGCACAAGCGCTGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCAAGAACAAAc                                                               >  1:319751/1‑251 (MQ=255)                                                                                                                                                                                                                              gtACATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGATTTTGGACATGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCAt  >  1:118654/1‑251 (MQ=255)                                                                                                                                                                                                                                 cATGANAGTTCNCNNGTTGAACCCACTATCAAAANGCTGGAAGGCGTGAANGGCGNCATTTNGAnc                                                                                                                                                                                        >  2:138634/1‑66 (MQ=255)                                                                                                                                                                                                                                 cATGACAGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAgctg                                                                                                                   <  1:138634/135‑1 (MQ=255)                                                                                                                                                                                                                                       aGTTCACTGGTTGAACCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAg                                                                                                         <  1:120989/139‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                    ACTGTAAAACGGCCATTGTGATGGTGCTGAAAAATCTCGATGCCGCAGAAGCTAAAAAACGCCTGGATCAACACGGCGGCTTTATTCGTCAGGTTTTAGACAAGGAATAACCCATGGCCAAAGAGATCAGCAGTGAACTTCTGAACACCATTCTTACCCGTGTCGGCGGACCGGGAAATATCGCCAGTTGTGGTAACTGTATGACGCGCCTGCGTCTGGGTGTACATGACAGTTCACTGGTTGATCCCAATATCAAAACGCTGGAAGGCGTGAAGGGCGTCATTTTGACCAGCGACCAGGTGCAGGTCGTTTTTGGGCCTGGTAAAGCGCATCGTGCCGCAAAAGCGATGAGCGAGCTGCTGGGAGAAGCACCGGTACAGGATGCCGCAGAAATCGCCGCCCAGAACAAACGTCAGTTAAAAGCCAAACAAACCTCCGGCGTGCAACAATTTCTCGCCAAATTCGCCACCAT  >  NC_000913/2546560‑2547031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑