Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,528,150	C→T	E4K (GAG→AAG)	ypeB ←	PF12843 family protein YpeB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,528,150	0	C	T	100.0%	39.7 / NA	15	E4K (GAG→AAG)	ypeB	PF12843 family protein YpeB
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (9/6);  total (9/6)

CCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTCTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGT  >  NC_000913/2527967‑2528330                                                                                                                                                                                        |                                                                                                                                                                                     ccAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCAACGCGCTTCCCTGCAGGGAACTCATCTTTGCGGGCACACCACAGCAGATAATCCTCCGGCAGGGCGATTAAGCGACGCCCTTTGTATTTGCCCAACGGCATTATCGTATTGGCTATTTCAATCTGCTGCTTTTTTTCCATCTCCGCTCCCCAGCAAACGCAGCCTTTCGGCTTCGTCGCTGACTTCAATGCCCAGTTc                                                                                                                   >  1:225706/1‑251 (MQ=255)         gTCAGCCCCTCGGTTTTAATCAGCAGGGTGATTTGCAATAGATCACCGAGCTTTCCCGCCGGGGACTCATATTTTCGGGCAAACCCCAGCCGATACACCTCAGGCAGGTCGATTAAGCGAAGCCCCTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTTCTTTTTTTTCATCTCAGCTAACCAGCCAACGCAGCATTTCCCCTTCGTCGATGACTTTAATGCCCCGTTCCTgccccc                                                                                                           >  1:163767/1‑247 (MQ=255)                 cTCGGTTTTAATCAGCAGGGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCCAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAAAGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGGCGGTGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGt                                                                                                   >  1:120809/1‑251 (MQ=255)                                    gTGATTTGCATTAGCTCACCGAGCTTCCCTGCCAGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCAc                                                                                <  1:271851/251‑1 (MQ=255)                                                aGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATACGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCAcc                                                                     >  1:204732/1‑250 (MQ=255)                                                      ncGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTc                                                                                                                   >  1:31623/2‑198 (MQ=255)                                                                     gggAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTGCCGCTTCGTCGATGACTTCAAGGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCTCTATCACCAGATCGGTTTTCTTCGACACGc                                               <  1:146479/251‑1 (MQ=255)                                                                              tCTTTGCGGGCAAACCACAGCAGATACGCCTCCGGCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGcc                                                                                                      <  1:238170/187‑1 (MQ=255)                                                                                   gCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGCTTCAGCGACGCCCTTTGTATTTGCCAAACGGCATTCTCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTc                                 >  1:351968/1‑251 (MQ=255)                                                                                                agcagATACTCCTCCGGCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCg                     >  1:161296/1‑250 (MQ=255)                                                                                                          catccGCCAGGTCGATTAAGCCACGACCTTTGCATTTTCCAAACGGCATTATCGTGTTGGCTATTTCAATCAGCTGCTTTTTTTCCATNNCAGCTAnnc                                                                                                                                                                  >  2:23095/3‑99 (MQ=255)                                                                                                               ggCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCAATCTCAGCTAc                                                                                                                                                                    >  1:227102/1‑92 (MQ=255)                                                                                                                  aGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACGTCAATGCCCAGTTGCTGCGCCTTCGTCAGTTTATATCCTGCAGCTTCGCCCGCTGTCACCAGATCGGTTTTCTCCGACGCGCTGCCCGCGACTGTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCgn  <  1:40925/251‑2 (MQ=255)                                                                                                                                   cccTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAATCGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTTCGCCTTCGCCATTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGc                                            <  1:50205/192‑1 (MQ=255)                                                                                                                                   cccTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTTTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCGAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGACCGGTTttcttc                                                      <  1:118691/182‑1 (MQ=255)                                                                                                                                                                                        |                                                                                                                                                                                     CCAGTTGCGTCAGCCCCTCGGTTTTAATCAGCAGCGTGATTTGCATTAGCTCACCGAGCTTCCCTGCCGGGAACTCATCTTTGCGGGCAAACCACAGCAGATACTCCTCCGGCAGGTCGATTAAGCGACGCCCTTTGTATTTGCCAAACGGCATTATCGTATTGGCTATTTCAATCAGCTGCTCTTTTTCCATCTCAGCTACCCAGCAAACGCAGCATTTCCGCTTCGTCGATGACTTCAATGCCCAGTTCCTGCGCCTTCGCCAGTTTAGATCCTGCAGCTTCACCCGCTATCACCAGATCGGTTTTCTTCGACACGCTGCCCGCGACTTTCGCGCCCAGTTCGACCAGTCGAGCTTTAGCGT  >  NC_000913/2527967‑2528330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑