Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,481,608	A→G	L190S (TTA→TCA)	emrY ←	tripartite efflux pump membrane subunit EmrY

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,481,608	0	A	G	100.0%	35.2 / NA	11	L190S (TTA→TCA)	emrY	tripartite efflux pump membrane subunit EmrY
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/2);  total (9/2)

CTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATCGCGCCCTTTATCAAGCATAATTTGCAAGCCACCAACACCGAGCACTAACAGGGTCAGTCCTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTG  >  NC_000913/2481397‑2481803                                                                                                                                                                                                                    |                                                                                                                                                                                                    ctctGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGACATAACTGATACTACTGTTAATATTACTATTGTACTCGAGTTGAACAAATCAAGATCGCGCCCTTTATCAAGCATAATTTGCAAGCCACCAACACCGAGCACTAACAGGGGCAGTCCTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTGAGCATAATGTCAGGACGATAATCCCCATAGGGACATTg                                                                                                                                                               >  1:264778/1‑250 (MQ=255)           gACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATCGCGCCCTTTATCAAGCATAATTTGCAAGCCACCAACACCGAGCACTAACAGGGTCAGTCCTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTGAGCATAATGTCAGGAc                                                                                                                                                                                     >  1:245639/1‑218 (MQ=255)                 cAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTCTTGTACTCGAGTTGAACCAATCCAGATCGCGCCCTTTATCAAGCATAATTTGCAAGCCACCAACACCGAGCACTAACAGGGTCAGTCCTGGTAGATCCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTGAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAAcc                                                                                                                                              >  1:353318/1‑251 (MQ=255)                                    gagaAATAACTGATACTACTGTTAATATTATTATTGAACTCGAGTTGAACCACTCCAGATCGCGCCCTTTATCAAGCATAATTTGCAAGCCACCAACACCGAGCACTAACAGGGTCAGTCCTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTGAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAg                                                                                                                                    >  1:125835/1‑242 (MQ=255)                                                                                                 gcgcCCTTTATCAAGCATAATTTGCAAGCCACCAACACCGAGCACTAACAGGGTCAGTCCTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCATTAAGTAAGGTTGAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATg                                                              <  1:358462/251‑1 (MQ=255)                                                                                                                        gCAAGCCACCAACACCGAGCACTAACAGGGTCAGTCCTGGAAGATTCATTTTGACCGGTGAAGTCTAAGTTTCTCTTACTTTAAGTAAGGTTGAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCTAACCCCAGCTAAAGATATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACAATAATGACAGAGCAAATGTTCTTTTTTCt                                       >  1:179203/1‑251 (MQ=255)                                                                                                                                                   gggTCAGTCCTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGAAAGGTTGAGCCTAATGTCAGGACGATAATCCCCATAGGGCCATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACCTCTCGGAGCGATAATCACGGTCATTGACCATACTGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAActc            >  1:259239/1‑251 (MQ=255)                                                                                                                                                            cTGGTAGATTCATTTTGACCGGTGACGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTGAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGccc                                                                                                               >  1:93769/1‑143 (MQ=255)                                                                                                                                                                    ttCATTTTGACCGGTGAAGGCTCAGTTTCTCTTCCTTTAAGTAAGGTTGAGCATAATGTCAGGACGATAATCCCCATAGGTACATTGATTAAAAATATCGAACCCCAGCTAAAGTTATCACAAATATAACCGCNCAANNNNNGcnnnnnn                                                                                                >  2:224091/1‑144 (MQ=255)                                                                                                                                                                             aCCGGCGAAATCTCAGTTTCTCTTCCTTTAAGGAAGGTTGAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATc                                                                                                                                                   >  1:113510/1‑90 (MQ=255)                                                                                                                                                                                                       tttAAGTAAGGTTGAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCATATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTg  <  1:122751/209‑1 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                    CTCTGAGGTCGACTCCCAAATGACTAAAGAGATCAGAGAAATAACTGATACTACTGTTAATATTATTATTGTACTCGAGTTGAACCAATCCAGATCGCGCCCTTTATCAAGCATAATTTGCAAGCCACCAACACCGAGCACTAACAGGGTCAGTCCTGGTAGATTCATTTTGACCGGTGAAGTCTCAGTTTCTCTTCCTTTAAGTAAGGTTAAGCATAATGTCAGGACGATAATCCCCATAGGGACATTGATTAAAAATATCCAACCCCAGCTAAAGTTATCACAAATATAACCGCCCAATATCGGCCCACATATCGGAGCGATAATCACGGTCATTGACCATAATGCCAGAGCAAATGTTCTTTTTTCTGGCGGATAATTCCTTAATAATAAACTCTGTGACAGTG  >  NC_000913/2481397‑2481803

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑