Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,388,789	C→T	P52L (CCG→CTG)	yfbO →	uncharacterized protein YfbO

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,388,789	0	C	T	100.0%	31.2 / NA	12	P52L (CCG→CTG)	yfbO	uncharacterized protein YfbO
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (5/7);  total (5/7)

CTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGC  >  NC_000913/2388578‑2389031                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   cTAATGAATTCAAACAATGTCATTCGTTTGATGAATATCTTATTTCAGGGAATAATTATGACACATTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCACTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCTGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCg                                                                                                                                                                                                             >  1:56312/1‑251 (MQ=255)                                                                   ttAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCTGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGt                                                                                                                                             <  1:336229/249‑1 (MQ=255)                                                                                        gTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCTGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTgg                                                                                                                                                      <  1:1882/219‑1 (MQ=255)                                                                                                                         aCTCCTCGTCTATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCGATATGTTGCAATGTGATTCCAGAACAATCTCTGCAAGCTATTTATTACCAGTTTCTGAAAATACGTTAGCGTAACAATGTTAGTGCTGTTTTGGTAGAAATTACTATTTTTGATGACTCTGTCTGGTTCGTTATTGAAAGTATTTTAGTTATCCCCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGt                                                                                     <  1:359958/251‑1 (MQ=255)                                                                                                                            cctcGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCTGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTan                                                                                   <  1:104999/250‑2 (MQ=255)                                                                                                                                              ctTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGGGATTCCAGAACAATCTCTGCAGGCTATCTATCACCATTTTCTGAAAATACGAGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCACTTTAGTGAAAGTATTTTAGTTATcaccac                                                                                                                      >  1:59726/1‑197 (MQ=255)                                                                                                                                                                  cAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCTGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCag                                            <  1:176172/251‑1 (MQ=255)                                                                                                                                                                      aataTCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCTGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGTGCCGTTAGTAAGAGTATTTTACTTATCACCACCGCTACACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAAGTGGCCCTGATGAGTGCTGGCAAGCATGGTCagaag                                         <  1:116987/250‑1 (MQ=255)                                                                                                                                                                                                     ttCCANAACAANCNNTGCAAGCTATCTATCACCANTTTCTGAAAATACGTNAGCGNAACAANGtn                                                                                                                                                                                                   >  2:147313/1‑64 (MQ=255)                                                                                                                                                                                                     ttCCAGAACAATCTCTGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTggg                                                        <  1:147313/204‑1 (MQ=255)                                                                                                                                                                                                      tCCAGAACAATCTCTGCAAGCTATCTATCACCATTTTCTGCAAATACGAGAGAGTACCAATGTTAGTGATGTTTTGGTAGAAATTACGCTGTTCGATGACACAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGACGTTCAGAGCTGGTTTGTAGAAGAACTTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTgg        >  1:323795/1‑251 (MQ=255)                                                                                                                                                                                                            aCAATCTCTGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAATTTACGATGTTCGATGACCCACACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCTGAGCTGGTTTGTAGAATATATTGCCCCTGATGAGTGCTGGGAAGGATGGTAAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGc  >  1:333282/1‑251 (MQ=255)                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   CTAATGAATTCAAACAATGTCATTCGTTTGATGAATATATTATTTCAGGGAATAATTATGACACCTTTAGAACGCATTACCCAACTTGTTAATATCAATGGTGATGTTAATAATCCTGATACTCCTCGTCCATTGTTATCTCTTGAGGATTTTTTTATTGACAATAATATCCATGGTTCAATATGTTGCAATGTGATTCCAGAACAATCTCCGCAAGCTATCTATCACCATTTTCTGAAAATACGTGAGCGTAACAATGTTAGTGATGTTTTGGTAGAAATTACGATGTTCGATGACCCAGACTGGCCCTTTAGTGAAAGTATTTTAGTTATCACCACTGCTTCACCAGAAGAAGTTCAGAGCTGGTTTGTAGAAGAAATTGCCCCTGATGAGTGCTGGGAAGGATGGTCAGAAGATACGGAGCATGGATGGGTTGAAGTCCCTGTGGGTATGC  >  NC_000913/2388578‑2389031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑