Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	2,361,210	C→G	E69Q (GAA→CAA)	rhmD ←	L‑rhamnonate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	2,361,210	0	C	G	100.0%	37.0 / NA	12	E69Q (GAA→CAA)	rhmD	L‑rhamnonate dehydratase
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (7/5);  total (7/5)

CCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGG  >  NC_000913/2360969‑2361451                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  ccGACCACTTTGCCGAACAGATCCCACAGAGCCAGATAGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACTGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAAcaacca                                                                                                                                                                                                                                           >  1:262817/1‑250 (MQ=255)                       cccACAGAGCCAGATCGACACCAGAAATCGTCTTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGcc                                                                                                                                                                                                                     >  1:111633/1‑250 (MQ=255)                                                                              ngAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAatat                                                                                                                                                             >  1:219668/2‑251 (MQ=255)                                                                                   aaTACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGCCACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGAATGCTCGTAATCGCGGTATTTACTCATCGGGGTGGc                                                                                                                                                                  >  1:179521/1‑241 (MQ=255)                                                                                                                                 aCTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACt                                                                                                                                                                                                             <  1:25992/152‑1 (MQ=255)                                                                                                                                                      gAAACGGTTAAGATGTTGTTCGTCATTAAAACAGCCCATTTCACCGGCTGTCGAAGCGGCGAATCCGGTCTGGCCGTTTTCTGCTGCGATTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAAGGACTGGCGTGACTGCTCGTAATCGCGGTATTTGCTCATCGGGTTTGCAATATGATCGTCTATCCAGTGATGCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCAc                                                                                     <  1:239168/251‑1 (MQ=255)                                                                                                                                                        aaCGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCg                                                                                   <  1:9766/251‑1 (MQ=255)                                                                                                                                                                                       ncccATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGCTCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACATGTTTAAttt                                                    >  1:70431/2‑251 (MQ=255)                                                                                                                                                                                       gCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTa                                                                                                                                                                                    >  1:16162/1‑123 (MQ=255)                                                                                                                                                                                                               ggctaaTCCGGTCTGGCCGTTTGCTGGTTCGACTTGAACAACCAACGTGCCAAGAACGTGAATTCCAAATGACTGGCGTGACTGCTCGGAATGGCGTTATTTTCTCAGCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAAt                                                                                                                      <  1:183066/157‑1 (MQ=255)                                                                                                                                                                                                                                  nnnnnnnnnncGNNNNNAACNACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAg                                                                                                          <  2:219668/144‑1 (MQ=255)                                                                                                                                                                                                                                       tGCTTCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCaa     >  1:296924/1‑250 (MQ=255)                                                                                                                                                                                                                                          ttCGACTTGAACAACCAACGTGCCAAGAACGTTAATGCAAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTAATAATCACCGCCGCCAGCGCCTTTTGCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAgg  <  1:114110/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                  CCGACCACTTTGCCGAACAGATCCCACAGAGCCAGATCGACACAAGAAATCGTATTCATCACCAGGCCACCAGAGCCGGAGTAATACAGGGTGGCACTGAGCATTTGATCGTGGATCAGTTTGATATCACTGACACATTTACCCTCAATGAAACGGTTAAGATGTTTTTCGACAATAAAACAGCCCATTTCACCGGCTGTCGAAACGGCGAATCCGGTCTGGCCGTTTTCTGCTTCGACTTCAACAACCAACGTGCCAAGAACGTTAATGCCAAATGACTGGCGTGACTGCTCGTAATCGCGGTATTTACTCATCGGGGTGGCAATATGATCGTCTATCCAGTGATTCGCCCCCTGGTCGTGATAATCACCGCCGCCAGCGCCTTTTTCTGCTGTCGCACCGCCAGTAAACCAGGCGCGAACCTGTTTAATTTTTGGTAGGGTCATGATGTTCTCCATTGTTATGAGGCTTGTAAGTCAAAGG  >  NC_000913/2360969‑2361451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑